ID   NA11_BUNCP              Reviewed;          43 AA.
AC   P0DTR3;
DT   13-NOV-2019, integrated into UniProtKB/Swiss-Prot.
DT   13-NOV-2019, sequence version 1.
DT   25-MAY-2022, entry version 7.
DE   RecName: Full=Delta-actitoxin-Bca1a {ECO:0000305};
DE            Short=Delta-AITX-Bca1a {ECO:0000303|PubMed:31330191};
OS   Bunodosoma capense (Knobbly sea anemone) (Actinia capensis).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC   Actiniidae; Bunodosoma.
OX   NCBI_TaxID=2022720;
RN   [1]
RP   PROTEIN SEQUENCE, MASS SPECTROMETRY, AND SUBCELLULAR LOCATION.
RX   PubMed=31330191; DOI=10.1016/j.toxicon.2019.06.222;
RA   van Losenoord W., Krause J., Parker-Nance S., Krause R., Stoychev S.,
RA   Frost C.L.;
RT   "Purification and biochemical characterisation of a putative sodium channel
RT   agonist secreted from the South African Knobbly sea anemone Bunodosoma
RT   capense.";
RL   Toxicon 168:147-157(2019).
CC   -!- FUNCTION: Binds specifically to voltage-gated sodium channels (Nav),
CC       thereby delaying their inactivation during signal transduction
CC       (PubMed:31330191). Thus it strongly stimulates mammalian cardiac muscle
CC       contraction (By similarity). {ECO:0000250|UniProtKB:P01529,
CC       ECO:0000305|PubMed:31330191}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:31330191}.
CC       Nematocyst {ECO:0000250|UniProtKB:P01529}.
CC   -!- MASS SPECTROMETRY: Mass=4619.7; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:31330191};
CC   -!- SIMILARITY: Belongs to the sea anemone sodium channel inhibitory toxin
CC       family. Type I subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; P0DTR3; -.
DR   SMR; P0DTR3; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR   GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0009966; P:regulation of signal transduction; IEA:InterPro.
DR   Gene3D; 2.20.20.10; -; 1.
DR   InterPro; IPR000693; Anenome_toxin.
DR   InterPro; IPR023355; Myo_ane_neurotoxin_sf.
DR   PIRSF; PIRSF001905; Anenome_toxin; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Nematocyst; Neurotoxin; Secreted; Toxin;
KW   Voltage-gated sodium channel impairing toxin.
FT   CHAIN           1..43
FT                   /note="Delta-actitoxin-Bca1a"
FT                   /evidence="ECO:0000269|PubMed:31330191"
FT                   /id="PRO_0000448543"
FT   DISULFID        1..41
FT                   /evidence="ECO:0000250|UniProtKB:P01528"
FT   DISULFID        3..31
FT                   /evidence="ECO:0000250|UniProtKB:P01528"
FT   DISULFID        24..42
FT                   /evidence="ECO:0000250|UniProtKB:P01528"
SQ   SEQUENCE   43 AA;  4629 MW;  D431E04EA23DB665 CRC64;
     CLCNSDGPSV RGNTLSGILW LAGCPSGWHN CKKHKPTIGW CCK