ID   NA12A_ANTS7             Reviewed;          47 AA.
AC   P0C5F4;
DT   02-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT   02-OCT-2007, sequence version 1.
DT   25-MAY-2022, entry version 39.
DE   RecName: Full=Delta-actitoxin-Aspp1a {ECO:0000303|PubMed:22683676};
DE            Short=Delta-AITX-Aspp1a {ECO:0000303|PubMed:22683676};
DE   AltName: Full=Toxin Hk2a {ECO:0000303|PubMed:14672713};
OS   Anthopleura sp. (strain 'Zhanjiang') (Sea anemone).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC   Actiniidae; Anthopleura.
OX   NCBI_TaxID=462333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], TOXIC DOSE, AND FUNCTION.
RC   TISSUE=Tentacle;
RX   PubMed=12782079; DOI=10.1016/s0041-0101(03)00033-3;
RA   Liu W.H., Wang L., Wang Y.L., Peng L.S., Wu W.Y., Peng W.L., Jiang X.Y.,
RA   Tu H.B., Chen H.P., Ou-Yang P., Xu A.L.;
RT   "Cloning and characterization of a novel neurotoxin from the sea anemone
RT   Anthopleura sp.";
RL   Toxicon 41:793-801(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
RC   TISSUE=Tentacle;
RX   PubMed=14672713; DOI=10.1016/j.bbrc.2003.11.102;
RA   Wang L., Ou J., Peng L., Zhong X., Du J., Liu Y., Huang Y., Liu W.,
RA   Zhang Y., Dong M., Xu A.-L.;
RT   "Functional expression and characterization of four novel neurotoxins from
RT   sea anemone Anthopleura sp.";
RL   Biochem. Biophys. Res. Commun. 313:163-170(2004).
RN   [3]
RP   NOMENCLATURE.
RX   PubMed=22683676; DOI=10.1016/j.toxicon.2012.05.020;
RA   Oliveira J.S., Fuentes-Silva D., King G.F.;
RT   "Development of a rational nomenclature for naming peptide and protein
RT   toxins from sea anemones.";
RL   Toxicon 60:539-550(2012).
CC   -!- FUNCTION: Binds specifically to voltage-gated sodium channels (Nav)
CC       (site 3), thereby delaying their inactivation during signal
CC       transduction (By similarity). Has a heart stimulation effect on
CC       isolated rat atria that is higher than that of Hk7a, Hk8a and Hk16a
CC       (PubMed:12782079,PubMed:14672713). {ECO:0000250,
CC       ECO:0000269|PubMed:12782079, ECO:0000269|PubMed:14672713}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}. Nematocyst {ECO:0000305}.
CC   -!- TOXIC DOSE: LD(50) is 1.4 mg/kg when intraperitoneally injected into
CC       mice. {ECO:0000269|PubMed:12782079}.
CC   -!- SIMILARITY: Belongs to the sea anemone sodium channel inhibitory toxin
CC       family. Type I subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; P0C5F4; -.
DR   SMR; P0C5F4; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR   GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0009966; P:regulation of signal transduction; IEA:InterPro.
DR   Gene3D; 2.20.20.10; -; 1.
DR   InterPro; IPR000693; Anenome_toxin.
DR   InterPro; IPR023355; Myo_ane_neurotoxin_sf.
DR   PIRSF; PIRSF001905; Anenome_toxin; 1.
PE   2: Evidence at transcript level;
KW   Cardiotoxin; Disulfide bond; Ion channel impairing toxin; Nematocyst;
KW   Neurotoxin; Secreted; Toxin; Voltage-gated sodium channel impairing toxin.
FT   CHAIN           1..47
FT                   /note="Delta-actitoxin-Aspp1a"
FT                   /id="PRO_0000305108"
FT   DISULFID        4..44
FT                   /evidence="ECO:0000250|UniProtKB:P01530"
FT   DISULFID        6..34
FT                   /evidence="ECO:0000250|UniProtKB:P01530"
FT   DISULFID        27..45
FT                   /evidence="ECO:0000250|UniProtKB:P01530"
SQ   SEQUENCE   47 AA;  4872 MW;  AFEAE3BB60A1D9CB CRC64;
     GVPCLCDSDG PSVRGNTLSG TLWLAGCPSG WHNCKAHGPT IGWCCKK