NA12_ANTFU
ID NA12_ANTFU Reviewed; 48 AA.
AC P10454;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 25-MAY-2022, entry version 80.
DE RecName: Full=Delta-actitoxin-Afv1b {ECO:0000303|PubMed:22683676};
DE Short=Delta-AITX-Afv1b {ECO:0000303|PubMed:22683676};
DE AltName: Full=AFT-II {ECO:0000303|PubMed:15169781, ECO:0000303|PubMed:2883740};
DE Short=AFII;
OS Anthopleura fuscoviridis (Sea anemone).
OC Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC Actiniidae; Anthopleura.
OX NCBI_TaxID=6111;
RN [1]
RP PROTEIN SEQUENCE, AND DISULFIDE BONDS.
RC TISSUE=Nematoblast;
RX PubMed=2883740; DOI=10.1016/0041-0101(87)90243-1;
RA Sunahara S., Muramoto K., Tenma K., Kamiya H.;
RT "Amino acid sequence of two sea anemone toxins from Anthopleura
RT fuscoviridis.";
RL Toxicon 25:211-219(1987).
RN [2]
RP FUNCTION, MASS SPECTROMETRY, AND TOXIC DOSE.
RX PubMed=15169781; DOI=10.1074/jbc.m404344200;
RA Oliveira J.S., Redaelli E., Zaharenko A.J., Cassulini R.R., Konno K.,
RA Pimenta D.C., Freitas J.C., Clare J.J., Wanke E.;
RT "Binding specificity of sea anemone toxins to Nav 1.1-1.6 sodium channels:
RT unexpected contributions from differences in the IV/S3-S4 outer loop.";
RL J. Biol. Chem. 279:33323-33335(2004).
RN [3]
RP ERRATUM OF PUBMED:15169781.
RA Oliveira J.S., Redaelli E., Zaharenko A.J., Cassulini R.R., Konno K.,
RA Pimenta D.C., Freitas J.C., Clare J.J., Wanke E.;
RL J. Biol. Chem. 279:44229-44230(2004).
RN [4]
RP NOMENCLATURE.
RX PubMed=22683676; DOI=10.1016/j.toxicon.2012.05.020;
RA Oliveira J.S., Fuentes-Silva D., King G.F.;
RT "Development of a rational nomenclature for naming peptide and protein
RT toxins from sea anemones.";
RL Toxicon 60:539-550(2012).
CC -!- FUNCTION: Binds specifically to voltage-gated sodium channels (Nav),
CC thereby delaying their inactivation. This toxin has a great efficacy
CC for Nav1.4/SCN4A (EC(50)=30.62 nM) and Nav1.5/SCN5A (EC(50)=62.5 nM).
CC It is less potent on Nav1.6/SCN8A (EC(50)=about 300 nM), Nav1.1/SCN1A
CC (EC(50)=390.55 nM), Nav1.3/SCN3A (EC(50)=459.36 nM) and Nav1.2/SCN2A
CC (EC(50)=1998.00 nM) (when measured as the increase in the slow
CC component). {ECO:0000269|PubMed:15169781}.
CC -!- SUBCELLULAR LOCATION: Secreted. Nematocyst.
CC -!- MASS SPECTROMETRY: Mass=4941; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:15169781};
CC -!- TOXIC DOSE: LD(50) is 450 ug/kg by intraperitoneal injection into mice.
CC {ECO:0000303|PubMed:15169781}.
CC -!- SIMILARITY: Belongs to the sea anemone sodium channel inhibitory toxin
CC family. Type I subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR PIR; B25860; B25860.
DR AlphaFoldDB; P10454; -.
DR SMR; P10454; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0009966; P:regulation of signal transduction; IEA:InterPro.
DR Gene3D; 2.20.20.10; -; 1.
DR InterPro; IPR000693; Anenome_toxin.
DR InterPro; IPR023355; Myo_ane_neurotoxin_sf.
DR PIRSF; PIRSF001905; Anenome_toxin; 1.
PE 1: Evidence at protein level;
KW Cardiotoxin; Direct protein sequencing; Disulfide bond;
KW Ion channel impairing toxin; Nematocyst; Neurotoxin; Secreted; Toxin;
KW Voltage-gated sodium channel impairing toxin.
FT CHAIN 1..48
FT /note="Delta-actitoxin-Afv1b"
FT /evidence="ECO:0000269|PubMed:2883740"
FT /id="PRO_0000221519"
FT DISULFID 5..45
FT /evidence="ECO:0000269|PubMed:2883740"
FT DISULFID 7..35
FT /evidence="ECO:0000269|PubMed:2883740"
FT DISULFID 28..46
FT /evidence="ECO:0000269|PubMed:2883740"
SQ SEQUENCE 48 AA; 4941 MW; 3CD4B66FADA1D999 CRC64;
GGVPCLCDSD GPSVRGNTLS GIIWLAGCPS GWHNCKAHGP TIGWCCKQ