ID   NA15_ANESU              Reviewed;          46 AA.
AC   P01529;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Delta-actitoxin-Avd1d {ECO:0000303|PubMed:22683676};
DE            Short=Delta-AITX-Avd1d {ECO:0000303|PubMed:22683676};
DE   AltName: Full=ATX-V;
DE   AltName: Full=As5 {ECO:0000303|PubMed:6127075};
DE   AltName: Full=Toxin V {ECO:0000303|PubMed:6127075};
OS   Anemonia sulcata (Mediterranean snakelocks sea anemone).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC   Actiniidae; Anemonia.
OX   NCBI_TaxID=6108;
RN   [1]
RP   PROTEIN SEQUENCE, AND FUNCTION.
RC   TISSUE=Nematoblast;
RX   PubMed=6127075; DOI=10.1016/0006-291x(82)91700-4;
RA   Scheffler J.-J., Tsugita A., Linden G., Schweitz H., Lazdunski M.;
RT   "The amino acid sequence of toxin V from Anemonia sulcata.";
RL   Biochem. Biophys. Res. Commun. 107:272-278(1982).
RN   [2]
RP   NOMENCLATURE.
RX   PubMed=22683676; DOI=10.1016/j.toxicon.2012.05.020;
RA   Oliveira J.S., Fuentes-Silva D., King G.F.;
RT   "Development of a rational nomenclature for naming peptide and protein
RT   toxins from sea anemones.";
RL   Toxicon 60:539-550(2012).
CC   -!- FUNCTION: Binds specifically to voltage-gated sodium channels (Nav),
CC       thereby delaying their inactivation during signal transduction. Thus it
CC       strongly stimulates mammalian cardiac muscle contraction.
CC       {ECO:0000269|PubMed:6127075}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000305}. Nematocyst {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the sea anemone sodium channel inhibitory toxin
CC       family. Type I subfamily. {ECO:0000305}.
CC   -!- CAUTION: Opinions are divided on whether Anemonia viridis (Forsskal,
CC       1775) and Anemonia sulcata (Pennant, 1777) are separate species.
CC       {ECO:0000305}.
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DR   PIR; A01792; TZAZ5.
DR   AlphaFoldDB; P01529; -.
DR   SMR; P01529; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR   GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0009966; P:regulation of signal transduction; IEA:InterPro.
DR   Gene3D; 2.20.20.10; -; 1.
DR   InterPro; IPR000693; Anenome_toxin.
DR   InterPro; IPR023355; Myo_ane_neurotoxin_sf.
DR   PIRSF; PIRSF001905; Anenome_toxin; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Nematocyst; Neurotoxin; Secreted; Toxin;
KW   Voltage-gated sodium channel impairing toxin.
FT   CHAIN           1..46
FT                   /note="Delta-actitoxin-Avd1d"
FT                   /evidence="ECO:0000269|PubMed:6127075"
FT                   /id="PRO_0000221514"
FT   DISULFID        4..44
FT                   /evidence="ECO:0000250|UniProtKB:P01528"
FT   DISULFID        6..34
FT                   /evidence="ECO:0000250|UniProtKB:P01528"
FT   DISULFID        27..45
FT                   /evidence="ECO:0000250|UniProtKB:P01528"
FT   VARIANT         39
FT                   /note="P -> G (in 50% of the molecules)"
SQ   SEQUENCE   46 AA;  4884 MW;  E5C3620FA1D9CB9B CRC64;
     GVPCLCDSDG PSVRGNTLSG ILWLAGCPSG WHNCKKHKPT IGWCCK