ID   NA22_HETCR              Reviewed;          48 AA.
AC   P30783;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1993, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Delta-stichotoxin-Hcr1b {ECO:0000303|PubMed:22683676};
DE            Short=Delta-SHTX-Hcr1b {ECO:0000303|PubMed:22683676};
DE   AltName: Full=Neurotoxin II {ECO:0000303|PubMed:2903746};
DE   AltName: Full=RTX-II;
DE   AltName: Full=Rm2;
OS   Heteractis crispa (Leathery sea anemone) (Radianthus macrodactylus).
OC   Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC   Stichodactylidae; Heteractis.
OX   NCBI_TaxID=175771;
RN   [1]
RP   PROTEIN SEQUENCE, AND SUBCELLULAR LOCATION.
RC   TISSUE=Nematoblast;
RX   PubMed=2903746;
RA   Zykova T.A., Kozlovskaya E.P., Elyakov G.V.;
RT   "Amino acid sequence of neurotoxin II from the sea anemone Radianthus
RT   macrodactylus.";
RL   Bioorg. Khim. 14:878-882(1988).
RN   [2]
RP   SEQUENCE REVISION TO 39-40.
RX   PubMed=1686683; DOI=10.1016/0041-0101(91)90205-6;
RA   Norton R.S.;
RT   "Structure and structure-function relationships of sea anemone proteins
RT   that interact with the sodium channel.";
RL   Toxicon 29:1051-1084(1991).
RN   [3]
RP   NOMENCLATURE.
RX   PubMed=22683676; DOI=10.1016/j.toxicon.2012.05.020;
RA   Oliveira J.S., Fuentes-Silva D., King G.F.;
RT   "Development of a rational nomenclature for naming peptide and protein
RT   toxins from sea anemones.";
RL   Toxicon 60:539-550(2012).
CC   -!- FUNCTION: Binds to site 3 of voltage-gated sodium channels and inhibits
CC       the inactivation process. {ECO:0000250|UniProtKB:P30832}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:2903746}.
CC       Nematocyst.
CC   -!- SIMILARITY: Belongs to the sea anemone sodium channel inhibitory toxin
CC       family. Type II subfamily. {ECO:0000305}.
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DR   PIR; JN0386; JN0386.
DR   AlphaFoldDB; P30783; -.
DR   SMR; P30783; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR   GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0009966; P:regulation of signal transduction; IEA:InterPro.
DR   Gene3D; 2.20.20.10; -; 1.
DR   InterPro; IPR000693; Anenome_toxin.
DR   InterPro; IPR023355; Myo_ane_neurotoxin_sf.
DR   PIRSF; PIRSF001905; Anenome_toxin; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW   Nematocyst; Neurotoxin; Secreted; Toxin;
KW   Voltage-gated sodium channel impairing toxin.
FT   CHAIN           1..48
FT                   /note="Delta-stichotoxin-Hcr1b"
FT                   /evidence="ECO:0000269|PubMed:2903746"
FT                   /id="PRO_0000221523"
FT   DISULFID        3..43
FT                   /evidence="ECO:0000250|UniProtKB:P19651"
FT   DISULFID        5..33
FT                   /evidence="ECO:0000250|UniProtKB:P19651"
FT   DISULFID        26..44
FT                   /evidence="ECO:0000250|UniProtKB:P19651"
SQ   SEQUENCE   48 AA;  5238 MW;  82E333772276B9BE CRC64;
     GTCKCDDDGP DVRTATFTGS TEFANCNESW EKCLAVYTPV ASCCRKKK