NA23_HETMG
ID NA23_HETMG Reviewed; 48 AA.
AC P08380;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1988, sequence version 1.
DT 25-MAY-2022, entry version 84.
DE RecName: Full=Delta-stichotoxin-Rpa1b {ECO:0000303|PubMed:22683676};
DE Short=Delta-SHTX-Rpa1b {ECO:0000303|PubMed:22683676};
DE AltName: Full=RpIII {ECO:0000303|PubMed:2885222};
DE AltName: Full=Toxin III;
OS Heteractis magnifica (Magnificent sea anemone) (Radianthus magnifica).
OC Eukaryota; Metazoa; Cnidaria; Anthozoa; Hexacorallia; Actiniaria;
OC Stichodactylidae; Heteractis.
OX NCBI_TaxID=38281;
RN [1]
RP PROTEIN SEQUENCE, AND SUBCELLULAR LOCATION.
RC TISSUE=Nematoblast;
RX PubMed=2885222; DOI=10.1016/0014-5793(87)81018-9;
RA Metrione R.M., Schweitz H., Walsh K.A.;
RT "The amino acid sequence of toxin RpIII from the sea anemone, Radianthus
RT paumotensis.";
RL FEBS Lett. 218:59-62(1987).
RN [2]
RP FUNCTION, AND TOXIC DOSE.
RX PubMed=2412579; DOI=10.1021/bi00335a025;
RA Schweitz H., Bidard J.-N., Frelin C., Pauron D., Vijverberg H.P.M.,
RA Mahasneh D.M., Lazdunski M., Vilbois F., Tsugita A.;
RT "Purification, sequence, and pharmacological properties of sea anemone
RT toxins from Radianthus paumotensis. A new class of sea anemone toxins
RT acting on the sodium channel.";
RL Biochemistry 24:3554-3561(1985).
RN [3]
RP NOMENCLATURE.
RX PubMed=22683676; DOI=10.1016/j.toxicon.2012.05.020;
RA Oliveira J.S., Fuentes-Silva D., King G.F.;
RT "Development of a rational nomenclature for naming peptide and protein
RT toxins from sea anemones.";
RL Toxicon 60:539-550(2012).
CC -!- FUNCTION: Binds specifically to voltage-gated sodium channels (Nav),
CC thereby delaying their inactivation during signal transduction
CC (PubMed:2412579). Its toxicity is greater than that of RpII (AC P01534)
CC (PubMed:2412579). {ECO:0000269|PubMed:2412579}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:2885222}.
CC Nematocyst.
CC -!- TOXIC DOSE: LD(50) is 53 ug/kg when intraperitoneally injected into
CC mice. {ECO:0000269|PubMed:2412579}.
CC -!- TOXIC DOSE: LD(50) is 2.4 ug/kg when intracutaneously injected into
CC mice. {ECO:0000269|PubMed:2412579}.
CC -!- TOXIC DOSE: LD(50) is 10 ug/kg when on crabs.
CC {ECO:0000269|PubMed:2412579}.
CC -!- MISCELLANEOUS: The C-terminal lysine was not positively identified.
CC -!- SIMILARITY: Belongs to the sea anemone sodium channel inhibitory toxin
CC family. Type II subfamily. {ECO:0000305}.
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DR PIR; A25827; TZAZR3.
DR AlphaFoldDB; P08380; -.
DR BMRB; P08380; -.
DR SMR; P08380; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0042151; C:nematocyst; IEA:UniProtKB-SubCell.
DR GO; GO:0017080; F:sodium channel regulator activity; IEA:UniProtKB-KW.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR GO; GO:0009966; P:regulation of signal transduction; IEA:InterPro.
DR Gene3D; 2.20.20.10; -; 1.
DR InterPro; IPR000693; Anenome_toxin.
DR InterPro; IPR023355; Myo_ane_neurotoxin_sf.
DR PIRSF; PIRSF001905; Anenome_toxin; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Ion channel impairing toxin;
KW Nematocyst; Neurotoxin; Secreted; Toxin;
KW Voltage-gated sodium channel impairing toxin.
FT CHAIN 1..48
FT /note="Delta-stichotoxin-Rpa1b"
FT /evidence="ECO:0000269|PubMed:2885222"
FT /id="PRO_0000221528"
FT DISULFID 3..43
FT /evidence="ECO:0000250|UniProtKB:P19651"
FT DISULFID 5..33
FT /evidence="ECO:0000250|UniProtKB:P19651"
FT DISULFID 26..44
FT /evidence="ECO:0000250|UniProtKB:P19651"
SQ SEQUENCE 48 AA; 5337 MW; 9D2C5DDBCFDFCAA9 CRC64;
GNCKCDDEGP NVRTAPLTGY VDLGYCNEGW EKCASYYSPI AECCRKKK
