NAA25_DROME
ID NAA25_DROME Reviewed; 948 AA.
AC Q9VDQ7; Q7YTX6;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 144.
DE RecName: Full=Phagocyte signaling-impaired protein;
DE AltName: Full=N-terminal acetyltransferase B complex subunit MDM20 homolog;
DE AltName: Full=N-terminal acetyltransferase B complex subunit NAA25 homolog;
GN Name=psidin {ECO:0000303|PubMed:17208189}; ORFNames=CG4845;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000305}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAQ22590.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Testis;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=17208189; DOI=10.1016/j.cub.2006.11.026;
RA Brennan C.A., Delaney J.R., Schneider D.S., Anderson K.V.;
RT "Psidin is required in Drosophila blood cells for both phagocytic
RT degradation and immune activation of the fat body.";
RL Curr. Biol. 17:67-72(2007).
CC -!- FUNCTION: Non-catalytic subunit of the NatB complex which catalyzes
CC acetylation of the N-terminal methionine residues of proteins beginning
CC with Met-Asp or Met-Glu (By similarity). Has 2 roles in the larval
CC immune response: required both for the phagocytic degradation of
CC internalized bacteria and for the induction of Defensin in the fat
CC body. Within the phagocytic blood cells, has a role in detection of
CC infection and activation of the humoral immune response. {ECO:0000250,
CC ECO:0000269|PubMed:17208189}.
CC -!- SUBUNIT: Component of the N-terminal acetyltransferase B (NatB)
CC complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Lysosome {ECO:0000269|PubMed:17208189}.
CC Note=Blood cell lysosomes. {ECO:0000269|PubMed:17208189}.
CC -!- TISSUE SPECIFICITY: Expressed in the fat body after immune challenge.
CC {ECO:0000269|PubMed:17208189}.
CC -!- DISRUPTION PHENOTYPE: Flies are deficient in the induction of Defensin
CC whether the infection was with a Gram-negative or Gram-positive micro-
CC organism. {ECO:0000269|PubMed:17208189}.
CC -!- SIMILARITY: Belongs to the MDM20/NAA25 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAQ22590.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AE014297; AAF55733.1; -; Genomic_DNA.
DR EMBL; BT010121; AAQ22590.1; ALT_FRAME; mRNA.
DR RefSeq; NP_650858.1; NM_142601.2.
DR AlphaFoldDB; Q9VDQ7; -.
DR SMR; Q9VDQ7; -.
DR BioGRID; 67375; 14.
DR IntAct; Q9VDQ7; 4.
DR STRING; 7227.FBpp0083238; -.
DR PaxDb; Q9VDQ7; -.
DR PRIDE; Q9VDQ7; -.
DR EnsemblMetazoa; FBtr0083829; FBpp0083238; FBgn0243511.
DR GeneID; 42389; -.
DR KEGG; dme:Dmel_CG4845; -.
DR CTD; 42389; -.
DR FlyBase; FBgn0243511; psidin.
DR VEuPathDB; VectorBase:FBgn0243511; -.
DR eggNOG; KOG2053; Eukaryota.
DR HOGENOM; CLU_008075_0_0_1; -.
DR InParanoid; Q9VDQ7; -.
DR OMA; ICRRMWA; -.
DR OrthoDB; 1022953at2759; -.
DR PhylomeDB; Q9VDQ7; -.
DR BioGRID-ORCS; 42389; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 42389; -.
DR PRO; PR:Q9VDQ7; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0243511; Expressed in embryonic/larval hemocyte (Drosophila) and 28 other tissues.
DR ExpressionAtlas; Q9VDQ7; baseline and differential.
DR Genevisible; Q9VDQ7; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:1990761; C:growth cone lamellipodium; IDA:FlyBase.
DR GO; GO:0005764; C:lysosome; IDA:UniProtKB.
DR GO; GO:0031416; C:NatB complex; IPI:FlyBase.
DR GO; GO:0043005; C:neuron projection; IDA:FlyBase.
DR GO; GO:0051015; F:actin filament binding; IDA:FlyBase.
DR GO; GO:0030042; P:actin filament depolymerization; IGI:FlyBase.
DR GO; GO:0002253; P:activation of immune response; IMP:FlyBase.
DR GO; GO:0007298; P:border follicle cell migration; IMP:FlyBase.
DR GO; GO:0006955; P:immune response; IMP:UniProtKB.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0017196; P:N-terminal peptidyl-methionine acetylation; IBA:GO_Central.
DR GO; GO:0043524; P:negative regulation of neuron apoptotic process; IMP:FlyBase.
DR GO; GO:0006911; P:phagocytosis, engulfment; IMP:UniProtKB.
DR GO; GO:0010592; P:positive regulation of lamellipodium assembly; IMP:FlyBase.
DR GO; GO:0097374; P:sensory neuron axon guidance; IMP:FlyBase.
DR InterPro; IPR019183; N-acetylTrfase_B_cplx_non-cat.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR22767:SF3; PTHR22767:SF3; 1.
DR Pfam; PF09797; NatB_MDM20; 1.
DR SUPFAM; SSF48452; SSF48452; 1.
DR PROSITE; PS50293; TPR_REGION; 1.
PE 2: Evidence at transcript level;
KW Antimicrobial; Immunity; Innate immunity; Lysosome; Reference proteome;
KW Repeat; TPR repeat.
FT CHAIN 1..948
FT /note="Phagocyte signaling-impaired protein"
FT /id="PRO_0000314625"
FT REPEAT 46..79
FT /note="TPR 1"
FT /evidence="ECO:0000255"
FT REPEAT 80..113
FT /note="TPR 2"
FT /evidence="ECO:0000255"
FT REPEAT 523..560
FT /note="TPR 3"
FT /evidence="ECO:0000255"
FT REGION 841..862
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 841..860
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 437
FT /note="T -> S (in Ref. 3; AAQ22590)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 948 AA; 109110 MW; 799238B481ED80ED CRC64;
MAQQQGMDSA LFERRLRPVY DNLEVGNNRK ALQESEKLLR KHPNLLCARA LKGLSLLRLG
RYDESHGCLQ TVAEEKPTDD STLQVLSFCY REMEQLDKIV ELYQHAVKQN PGNEELLAHL
FISHVRVEDY KAQQAVALQL YKAQPKNAYY FWSVISVVFQ GIRGPESALP EKRKIYLGLA
QRMVDKHIKE GKMETEQEAF LYLHILKLQN KYQEAWEFLT GELCAKLYPG APVSMKFELL
KELGNWRELN ELLQQLLDAD RDRWDFYKEY IQSSFELLKL TPQGVENGDK DSLARCQEFL
QRIIDSSERK KRGPYLARLE LHQRMRAEQL PAEKLIGDFD EMVIEYFRLF GDKSCCTHDI
ALFLPSISMN QRQALASKLL LESGVTSTSL PKNKEQLQKH LCALQISRMC GSHMDLPADH
LLAFYTALKL HYEHGRTTFG KKLLATEMGP SDAYALLAAN VMYDLSRREN KSDHLFEALC
LLQYVLRNST SNFHVKLLSL KIYHLFGCQV GAQEMYEYLD IKQIQLDSMG YVHCQLLALG
GRFSGNRNVY DATLKFFTNS YKERLEYIAL TYRFCTFSKM EEFMNFKERL TNSLQYVACS
VEAQICDLVS CYGNITQNLS AYVAMSIEPA EDRIAWHELS DNRDLGAIIR WDPLHEVDAA
EERRESFDQE VEVLQVRSLM LRLFASFIDL YHGAGLKDSP AGAETATLEL LRESWTGLFQ
RLRVMNYKPL AQKFLVNLLP TRLHLLLDMP YERFFDDLAQ LVLDLQSGSA KLAEQCKRVG
DNVIATMELC VQTINESNES NGIDGLWKRR GQQQKVAASL EMLSLYAFLL SVLNDKLQVS
SKTKTKKKPV ENKVDGPQPI SEKERSQMLQ ELMRQLKHKF EACDTAIRSW KAPVLPRDLS
SFMSDMSLKP EVEATLIGDV SATFKDSHEL MVTELRNLLK DKIRMVAK
