NAA25_MOUSE
ID NAA25_MOUSE Reviewed; 972 AA.
AC Q8BWZ3; A1A4B7; Q3KQI7; Q3TJC6; Q7TMM2;
DT 10-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=N-alpha-acetyltransferase 25, NatB auxiliary subunit;
DE AltName: Full=Mitochondrial distribution and morphology protein 20;
DE AltName: Full=N-terminal acetyltransferase B complex subunit MDM20;
DE Short=NatB complex subunit MDM20;
DE AltName: Full=N-terminal acetyltransferase B complex subunit NAA25;
GN Name=Naa25; Synonyms=Mdm20;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Bone marrow, Embryonic stem cell, and Placenta;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=FVB/N; TISSUE=Brain, Embryo, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Non-catalytic subunit of the NatB complex which catalyzes
CC acetylation of the N-terminal methionine residues of peptides beginning
CC with Met-Asp-Glu. May play a role in normal cell-cycle progression.
CC {ECO:0000250}.
CC -!- SUBUNIT: Component of the N-terminal acetyltransferase B (NatB) complex
CC which is composed of NAA20 and NAA25. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8BWZ3-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BWZ3-2; Sequence=VSP_026631;
CC -!- SIMILARITY: Belongs to the MDM20/NAA25 family. {ECO:0000305}.
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DR EMBL; AK049325; BAC33684.1; -; mRNA.
DR EMBL; AK149758; BAE29066.1; -; mRNA.
DR EMBL; AK167405; BAE39494.1; -; mRNA.
DR EMBL; AK167491; BAE39569.1; -; mRNA.
DR EMBL; BC028868; AAH28868.1; -; mRNA.
DR EMBL; BC055480; AAH55480.1; -; mRNA.
DR EMBL; BC106182; AAI06183.1; -; mRNA.
DR EMBL; BC132172; AAI32173.1; -; mRNA.
DR CCDS; CCDS19633.1; -. [Q8BWZ3-1]
DR RefSeq; NP_766310.2; NM_172722.3. [Q8BWZ3-1]
DR AlphaFoldDB; Q8BWZ3; -.
DR SMR; Q8BWZ3; -.
DR BioGRID; 231159; 3.
DR IntAct; Q8BWZ3; 1.
DR STRING; 10090.ENSMUSP00000038977; -.
DR iPTMnet; Q8BWZ3; -.
DR PhosphoSitePlus; Q8BWZ3; -.
DR SwissPalm; Q8BWZ3; -.
DR EPD; Q8BWZ3; -.
DR MaxQB; Q8BWZ3; -.
DR PaxDb; Q8BWZ3; -.
DR PeptideAtlas; Q8BWZ3; -.
DR PRIDE; Q8BWZ3; -.
DR ProteomicsDB; 286137; -. [Q8BWZ3-1]
DR ProteomicsDB; 286138; -. [Q8BWZ3-2]
DR Antibodypedia; 50891; 118 antibodies from 20 providers.
DR DNASU; 231713; -.
DR Ensembl; ENSMUST00000042163; ENSMUSP00000038977; ENSMUSG00000042719. [Q8BWZ3-1]
DR GeneID; 231713; -.
DR KEGG; mmu:231713; -.
DR UCSC; uc008zjb.1; mouse. [Q8BWZ3-2]
DR UCSC; uc008zjc.1; mouse. [Q8BWZ3-1]
DR CTD; 80018; -.
DR MGI; MGI:2442563; Naa25.
DR VEuPathDB; HostDB:ENSMUSG00000042719; -.
DR eggNOG; KOG2053; Eukaryota.
DR GeneTree; ENSGT00950000183174; -.
DR HOGENOM; CLU_008075_0_0_1; -.
DR InParanoid; Q8BWZ3; -.
DR OMA; ICRRMWA; -.
DR PhylomeDB; Q8BWZ3; -.
DR TreeFam; TF315103; -.
DR BRENDA; 2.3.1.254; 3474.
DR BioGRID-ORCS; 231713; 27 hits in 70 CRISPR screens.
DR ChiTaRS; Naa25; mouse.
DR PRO; PR:Q8BWZ3; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q8BWZ3; protein.
DR Bgee; ENSMUSG00000042719; Expressed in spermatid and 228 other tissues.
DR ExpressionAtlas; Q8BWZ3; baseline and differential.
DR Genevisible; Q8BWZ3; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0005794; C:Golgi apparatus; ISO:MGI.
DR GO; GO:0031416; C:NatB complex; ISO:MGI.
DR GO; GO:0017196; P:N-terminal peptidyl-methionine acetylation; ISO:MGI.
DR InterPro; IPR019183; N-acetylTrfase_B_cplx_non-cat.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR22767:SF3; PTHR22767:SF3; 1.
DR Pfam; PF09797; NatB_MDM20; 1.
DR SUPFAM; SSF48452; SSF48452; 1.
DR PROSITE; PS50293; TPR_REGION; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Reference proteome; Repeat; TPR repeat.
FT CHAIN 1..972
FT /note="N-alpha-acetyltransferase 25, NatB auxiliary
FT subunit"
FT /id="PRO_0000294338"
FT REPEAT 11..44
FT /note="TPR 1"
FT REPEAT 45..78
FT /note="TPR 2"
FT REPEAT 79..112
FT /note="TPR 3"
FT REPEAT 114..146
FT /note="TPR 4"
FT VAR_SEQ 1..48
FT /note="MATRGHVQDPNDRRLRPIYDYLDNGNNKMAIQQADKLLKKHKDLHCAK ->
FT MCRTLTTGASGPFT (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_026631"
FT CONFLICT 424
FT /note="L -> I (in Ref. 1; BAE39569/BAE39494)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 972 AA; 111708 MW; 1393A5B38DE26D54 CRC64;
MATRGHVQDP NDRRLRPIYD YLDNGNNKMA IQQADKLLKK HKDLHCAKVL KAIGLQRTGK
QEEAFTLAQE VAALEPTDDN SLQALTILYR EMHRPELVTK LYEAAVKKVP NSEEYHSHLF
MAYARVGEYK KMQQAGMALY KIVPKNPYYF WSVMSLIMQS ISARDENLSK TMFLPLAERM
VEKMVKEDKI EAEAEVELYY MILERLGKYQ EALDVIRGKL GEKLTSEIQS RENKCMAMYK
KLSKWPECNA LSRRLLLKNS DDWQFYLTYF DSVFRLIEEA WTPPAEGEHS LEGEVHCSAE
DAVKFIEDRI TEASQSSRHV RGPHLAKLEL IRRLRSQGCN DEYKLGDPEE LMFQYFKKFG
DKPCCFTDLK VFVDLLPAAQ CTQFINQLLG VVPLSTPTED KLALPADIRG LQQHLCVVQL
TRLLGLYHSM DKSQKLDVVK ELMLRYQHGL EFGRSCLKTE LQFSDYYCLL AVHVLIDVWR
EAGEETAVWQ ALTLLEEGLT HSPSNAQFKL LLVRIYCVLG AFEPVVDLYS SLDAKHIQHD
TIGYLLTRYA ASLGQYAAAS QSCNFALRFF HSNQKDTSEY IIQAYKYGAF EKIPEFIAFR
NRLNNSLHFA QVRTERMLLD LLLEANISIS LAESIKSMNL RPEEDDVPWE DLRDNRDLDV
FFSWDPKDRN VSEEHKKLSL EEETMWLRIR SLTLRLISGL PSLTHPVEPK NSEKMSENGV
SSRIDILRLL LQQLEVAVET GKRFIEKEIQ YPFLGPVPTR MGRFFSSGCC QCQVQSFHLV
SDMYELDTSG LEGTVDIQER IENSLASLLE LLKGVFSTCK GDLLEVTDGN VKTQPAVLEN
LVFFVETISV ILWVSSYCES VLRPYKLNIQ KKKKKKKETS IIMPPIFTSF QDYVTGLQTV
ISNAVDHIKG LEAHLIALRL EELTLEETSI STEERKFSKT VQGKVQSSYL HSLLETGELL
RKRLETTKKL KI
