NAA35_DROME
ID NAA35_DROME Reviewed; 784 AA.
AC Q9W1A2; Q59E64; Q8T3X5;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=N-alpha-acetyltransferase 35, NatC auxiliary subunit homolog;
DE AltName: Full=Embryonic growth-associated protein homolog;
DE AltName: Full=Protein MAK10 homolog;
GN Name=Naa35 {ECO:0000312|FlyBase:FBgn0034982}; ORFNames=CG4065;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING (ISOFORMS A AND B).
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS A AND B).
RC STRAIN=Berkeley; TISSUE=Ovary, and Testis;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP IDENTIFICATION.
RX PubMed=16484612; DOI=10.1161/01.res.0000214539.86593.7a;
RA Wenzlau J.M., Garl P.J., Simpson P., Stenmark K.R., West J., Artinger K.B.,
RA Nemenoff R.A., Weiser-Evans M.C.M.;
RT "Embryonic growth-associated protein is one subunit of a novel N-terminal
RT acetyltransferase complex essential for embryonic vascular development.";
RL Circ. Res. 98:846-855(2006).
CC -!- FUNCTION: Probable auxillary component of a complex displaying alpha
CC (N-terminal) acetyltransferase activity. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A;
CC IsoId=Q9W1A2-1; Sequence=Displayed;
CC Name=B;
CC IsoId=Q9W1A2-2; Sequence=VSP_029040;
CC -!- SIMILARITY: Belongs to the MAK10 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL90190.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AE013599; AAX52681.1; -; Genomic_DNA.
DR EMBL; AE013599; AAF47171.1; -; Genomic_DNA.
DR EMBL; AY060948; AAL28496.1; -; mRNA.
DR EMBL; AY089452; AAL90190.1; ALT_FRAME; mRNA.
DR RefSeq; NP_001014546.1; NM_001014546.2. [Q9W1A2-2]
DR RefSeq; NP_611891.1; NM_138047.3. [Q9W1A2-1]
DR AlphaFoldDB; Q9W1A2; -.
DR SMR; Q9W1A2; -.
DR BioGRID; 63444; 3.
DR IntAct; Q9W1A2; 1.
DR STRING; 7227.FBpp0072187; -.
DR PaxDb; Q9W1A2; -.
DR PRIDE; Q9W1A2; -.
DR DNASU; 37866; -.
DR EnsemblMetazoa; FBtr0072280; FBpp0072187; FBgn0034982. [Q9W1A2-1]
DR EnsemblMetazoa; FBtr0100398; FBpp0099812; FBgn0034982. [Q9W1A2-2]
DR GeneID; 37866; -.
DR KEGG; dme:Dmel_CG4065; -.
DR UCSC; CG4065-RA; d. melanogaster. [Q9W1A2-1]
DR CTD; 60560; -.
DR FlyBase; FBgn0034982; Naa35.
DR VEuPathDB; VectorBase:FBgn0034982; -.
DR eggNOG; KOG2343; Eukaryota.
DR GeneTree; ENSGT00390000002445; -.
DR InParanoid; Q9W1A2; -.
DR OMA; ACLNRCR; -.
DR PhylomeDB; Q9W1A2; -.
DR SignaLink; Q9W1A2; -.
DR BioGRID-ORCS; 37866; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 37866; -.
DR PRO; PR:Q9W1A2; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0034982; Expressed in eye disc (Drosophila) and 35 other tissues.
DR ExpressionAtlas; Q9W1A2; baseline and differential.
DR Genevisible; Q9W1A2; DM.
DR GO; GO:0005737; C:cytoplasm; ISS:UniProtKB.
DR GO; GO:0031417; C:NatC complex; IBA:GO_Central.
DR GO; GO:0017196; P:N-terminal peptidyl-methionine acetylation; IEA:InterPro.
DR GO; GO:0006474; P:N-terminal protein amino acid acetylation; IBA:GO_Central.
DR InterPro; IPR007244; Naa35/Mak10.
DR PANTHER; PTHR21373; PTHR21373; 1.
DR Pfam; PF04112; Mak10; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; Reference proteome.
FT CHAIN 1..784
FT /note="N-alpha-acetyltransferase 35, NatC auxiliary subunit
FT homolog"
FT /id="PRO_0000308621"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 320..353
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 606..630
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 9..23
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 18
FT /note="Missing (in isoform B)"
FT /evidence="ECO:0000303|PubMed:12537569"
FT /id="VSP_029040"
SQ SEQUENCE 784 AA; 89204 MW; DBDB966F0F523F17 CRC64;
MYPSTLPAEA DPSTMNGSSV AEPPDFQAAT AAAAAAARAS SLEQDEWSVG ECGFLDPDVQ
RTMRSGSAAE DITQYPMHGW VDVTKEFHDA CAELQPGELA QDMLFGLFEA MSAIEIMDPK
MDVGMGFDKQ DLPPPSFEAA IATGAIKLDD LTPSELIGIY DALFSCLVSW LEGNSMDQVL
FTCLYLHAPA QIKDKALRVF CTAVRNLIVV IKKIIAVAAV NEEEDFQLYG NSALLAAEKA
QPATVYSSLK DVEDELIRKC KKLTSTEDWM AVVHRLRFMR HLFQVIYHVE QMASNDTVDD
KVDIYKILLV ASEMLPGIRN TLDRGTQPEK GSDAPNPMGF SPRIHDRSQP PAFPRSIKIR
DRPSSYQFLE EMISRFKYAC KVTKYKDYYS ALNFFIEYSK KSGQCILSRS VLQTLFSANM
RMAHGKLPMK QFLRHSVQVF NSPPVLNAKH PVAADPKVQQ HLENFFRYCI NMNTFTQFIR
ICGFNRARQR DKLARLIENF DTIQVDAARL DSMMNQLANE RAMEGNEPMA TALKHSTHFS
TWVLYNCFRA MLIFLMSGFE LELYAVHEFL YIYWYPYEFL IGFLVSALTR TENILLAQEE
YAEHQSKTQS GGSGAAKNRK AAKPKKNKKT QRPYRAEIVF YHALLSLCGG MYKAMGALTK
DGRVRLPLSK FDNEEIRYNR RFLPFATLTS PPPVSYAEFK NVREHMMRHS VEDLYTYAAK
HFDQARNVLE SIQNPDQEML DLLQIARTNF VVMNVLARGH QKEVKRQPEF DFSKHSYFPI
IKLK