NAA35_HUMAN
ID NAA35_HUMAN Reviewed; 725 AA.
AC Q5VZE5; Q5VZE6; Q9H631; Q9H703;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=N-alpha-acetyltransferase 35, NatC auxiliary subunit;
DE AltName: Full=Embryonic growth-associated protein homolog;
DE AltName: Full=Protein MAK10 homolog;
GN Name=NAA35; Synonyms=EGAP, MAK10;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Colon, and Small intestine;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164053; DOI=10.1038/nature02465;
RA Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L.,
RA Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R.,
RA Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S.,
RA Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K.,
RA Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C.,
RA Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E.,
RA Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M.,
RA Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J.,
RA Frankish A., Frankland J.A., French L., Fricker D.G., Garner P.,
RA Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S.,
RA Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E.,
RA McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V.,
RA Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S.,
RA Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K.,
RA Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J.,
RA Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M.,
RA West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L.,
RA Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J.,
RA Dunham I.;
RT "DNA sequence and analysis of human chromosome 9.";
RL Nature 429:369-374(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP IDENTIFICATION.
RX PubMed=16484612; DOI=10.1161/01.res.0000214539.86593.7a;
RA Wenzlau J.M., Garl P.J., Simpson P., Stenmark K.R., West J., Artinger K.B.,
RA Nemenoff R.A., Weiser-Evans M.C.M.;
RT "Embryonic growth-associated protein is one subunit of a novel N-terminal
RT acetyltransferase complex essential for embryonic vascular development.";
RL Circ. Res. 98:846-855(2006).
RN [6]
RP NOMENCLATURE.
RX PubMed=19660095; DOI=10.1186/1753-6561-3-s6-s2;
RA Polevoda B., Arnesen T., Sherman F.;
RT "A synopsis of eukaryotic Nalpha-terminal acetyltransferases: nomenclature,
RT subunits and substrates.";
RL BMC Proc. 3:S2-S2(2009).
RN [7]
RP FUNCTION IN APOPTOSIS, IDENTIFICATION IN NATC COMPLEX, AND SUBCELLULAR
RP LOCATION.
RX PubMed=19398576; DOI=10.1128/mcb.01909-08;
RA Starheim K.K., Gromyko D., Evjenth R., Ryningen A., Varhaug J.E.,
RA Lillehaug J.R., Arnesen T.;
RT "Knockdown of human N alpha-terminal acetyltransferase complex C leads to
RT p53-dependent apoptosis and aberrant human Arl8b localization.";
RL Mol. Cell. Biol. 29:3569-3581(2009).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-187, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- FUNCTION: Auxillary component of the N-terminal acetyltransferase C
CC (NatC) complex which catalyzes acetylation of N-terminal methionine
CC residues. Involved in regulation of apoptosis and proliferation of
CC smooth muscle cells. {ECO:0000269|PubMed:19398576}.
CC -!- SUBUNIT: Component of the N-terminal acetyltransferase C (NatC)
CC complex, which is composed of NAA35, NAA38 and NAA30.
CC {ECO:0000269|PubMed:19398576}.
CC -!- INTERACTION:
CC Q5VZE5; Q147X3: NAA30; NbExp=2; IntAct=EBI-9106478, EBI-9106461;
CC Q5VZE5; Q9C029: TRIM7; NbExp=3; IntAct=EBI-9106478, EBI-2813981;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:19398576}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5VZE5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5VZE5-2; Sequence=VSP_056098, VSP_056099;
CC -!- SIMILARITY: Belongs to the MAK10 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB15097.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAB15435.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK025266; BAB15097.1; ALT_FRAME; mRNA.
DR EMBL; AK026296; BAB15435.1; ALT_INIT; mRNA.
DR EMBL; AK056059; BAG51612.1; -; mRNA.
DR EMBL; AL161447; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL353743; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AL161453; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471089; EAW62702.1; -; Genomic_DNA.
DR EMBL; CH471089; EAW62703.1; -; Genomic_DNA.
DR EMBL; BC117427; AAI17428.1; -; mRNA.
DR EMBL; BC117429; AAI17430.1; -; mRNA.
DR CCDS; CCDS6673.1; -. [Q5VZE5-1]
DR RefSeq; NP_001308810.1; NM_001321881.1. [Q5VZE5-1]
DR RefSeq; NP_001308811.1; NM_001321882.1. [Q5VZE5-1]
DR RefSeq; NP_078911.3; NM_024635.3. [Q5VZE5-1]
DR RefSeq; XP_005252184.1; XM_005252127.3. [Q5VZE5-1]
DR AlphaFoldDB; Q5VZE5; -.
DR SMR; Q5VZE5; -.
DR BioGRID; 121941; 32.
DR ComplexPortal; CPX-6275; NatC N-alpha-acetyltransferase complex.
DR IntAct; Q5VZE5; 5.
DR STRING; 9606.ENSP00000354972; -.
DR iPTMnet; Q5VZE5; -.
DR MetOSite; Q5VZE5; -.
DR PhosphoSitePlus; Q5VZE5; -.
DR BioMuta; NAA35; -.
DR DMDM; 74747795; -.
DR EPD; Q5VZE5; -.
DR jPOST; Q5VZE5; -.
DR MassIVE; Q5VZE5; -.
DR MaxQB; Q5VZE5; -.
DR PaxDb; Q5VZE5; -.
DR PeptideAtlas; Q5VZE5; -.
DR PRIDE; Q5VZE5; -.
DR ProteomicsDB; 65692; -. [Q5VZE5-1]
DR ProteomicsDB; 65693; -.
DR Antibodypedia; 13206; 102 antibodies from 21 providers.
DR DNASU; 60560; -.
DR Ensembl; ENST00000361671.10; ENSP00000354972.5; ENSG00000135040.16. [Q5VZE5-1]
DR Ensembl; ENST00000376040.2; ENSP00000365208.1; ENSG00000135040.16. [Q5VZE5-2]
DR GeneID; 60560; -.
DR KEGG; hsa:60560; -.
DR MANE-Select; ENST00000361671.10; ENSP00000354972.5; NM_024635.4; NP_078911.3.
DR UCSC; uc004aoi.5; human. [Q5VZE5-1]
DR CTD; 60560; -.
DR DisGeNET; 60560; -.
DR GeneCards; NAA35; -.
DR HGNC; HGNC:24340; NAA35.
DR HPA; ENSG00000135040; Low tissue specificity.
DR MIM; 619438; gene.
DR neXtProt; NX_Q5VZE5; -.
DR OpenTargets; ENSG00000135040; -.
DR PharmGKB; PA165585932; -.
DR VEuPathDB; HostDB:ENSG00000135040; -.
DR eggNOG; KOG2343; Eukaryota.
DR GeneTree; ENSGT00390000002445; -.
DR HOGENOM; CLU_011757_1_1_1; -.
DR InParanoid; Q5VZE5; -.
DR OMA; ACLNRCR; -.
DR OrthoDB; 1370304at2759; -.
DR PhylomeDB; Q5VZE5; -.
DR TreeFam; TF320627; -.
DR BioCyc; MetaCyc:ENSG00000135040-MON; -.
DR PathwayCommons; Q5VZE5; -.
DR Reactome; R-HSA-6811440; Retrograde transport at the Trans-Golgi-Network.
DR SignaLink; Q5VZE5; -.
DR SIGNOR; Q5VZE5; -.
DR BioGRID-ORCS; 60560; 317 hits in 1093 CRISPR screens.
DR ChiTaRS; NAA35; human.
DR GenomeRNAi; 60560; -.
DR Pharos; Q5VZE5; Tbio.
DR PRO; PR:Q5VZE5; -.
DR Proteomes; UP000005640; Chromosome 9.
DR RNAct; Q5VZE5; protein.
DR Bgee; ENSG00000135040; Expressed in secondary oocyte and 208 other tissues.
DR Genevisible; Q5VZE5; HS.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0031417; C:NatC complex; IDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0005886; C:plasma membrane; IDA:HPA.
DR GO; GO:0017196; P:N-terminal peptidyl-methionine acetylation; IC:ComplexPortal.
DR GO; GO:0006474; P:N-terminal protein amino acid acetylation; IBA:GO_Central.
DR GO; GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB.
DR GO; GO:0048659; P:smooth muscle cell proliferation; ISS:UniProtKB.
DR InterPro; IPR007244; Naa35/Mak10.
DR PANTHER; PTHR21373; PTHR21373; 1.
DR Pfam; PF04112; Mak10; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Phosphoprotein; Reference proteome.
FT CHAIN 1..725
FT /note="N-alpha-acetyltransferase 35, NatC auxiliary
FT subunit"
FT /id="PRO_0000308614"
FT REGION 548..573
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 187
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VAR_SEQ 293..294
FT /note="DH -> GL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_056098"
FT VAR_SEQ 295..725
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_056099"
FT CONFLICT 229
FT /note="Q -> R (in Ref. 1; BAB15097)"
FT /evidence="ECO:0000305"
FT CONFLICT 487
FT /note="C -> W (in Ref. 1; BAB15097)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 725 AA; 83639 MW; A5612BA0CE1BAE3F CRC64;
MVMKASVDDD DSGWELSMPE KMEKSNTNWV DITQDFEEAC RELKLGELLH DKLFGLFEAM
SAIEMMDPKM DAGMIGNQVN RKVLNFEQAI KDGTIKIKDL TLPELIGIMD TCFCCLITWL
EGHSLAQTVF TCLYIHNPDF IEDPAMKAFA LGILKICDIA REKVNKAAVF EEEDFQSMTY
GFKMANSVTD LRVTGMLKDV EDDMQRRVKS TRSRQGEERD PEVELEHQQC LAVFSRVKFT
RVLLTVLIAF TKKETSAVAE AQKLMVQAAD LLSAIHNSLH HGIQAQNDTT KGDHPIMMGF
EPLVNQRLLP PTFPRYAKII KREEMVNYFA RLIDRIKTVC EVVNLTNLHC ILDFFCEFSE
QSPCVLSRSL LQTTFLVDNK KVFGTHLMQD MVKDALRSFV SPPVLSPKCY LYNNHQAKDC
IDSFVTHCVR PFCSLIQIHG HNRARQRDKL GHILEEFATL QDEAEKVDAA LHTMLLKQEP
QRQHLACLGT WVLYHNLRIM IQYLLSGFEL ELYSMHEYYY IYWYLSEFLY AWLMSTLSRA
DGSQMAEERI MEEQQKGRSS KKTKKKKKVR PLSREITMSQ AYQNMCAGMF KTMVAFDMDG
KVRKPKFELD SEQVRYEHRF APFNSVMTPP PVHYLQFKEM SDLNKYSPPP QSPELYVAAS
KHFQQAKMIL ENIPNPDHEV NRILKVAKPN FVVMKLLAGG HKKESKVPPE FDFSAHKYFP
VVKLV