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NAAA_BRASZ
ID   NAAA_BRASZ              Reviewed;         425 AA.
AC   D3WZ85;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   20-APR-2010, sequence version 1.
DT   03-AUG-2022, entry version 35.
DE   RecName: Full=5-nitroanthranilic acid aminohydrolase;
DE            EC=3.5.99.8;
DE   AltName: Full=5-nitroanthranilic acid degradation protein A;
DE   AltName: Full=5NAA deaminase;
GN   Name=naaA;
OS   Bradyrhizobium sp.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium; unclassified Bradyrhizobium.
OX   NCBI_TaxID=376;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=JS329;
RX   PubMed=20081004; DOI=10.1128/aem.02816-09;
RA   Qu Y., Spain J.C.;
RT   "Biodegradation of 5-Nitroanthranilic Acid by Bradyrhizobium sp. Strain
RT   JS329.";
RL   Appl. Environ. Microbiol. 76:1417-1422(2010).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, COFACTOR, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC   STRAIN=JS329;
RX   PubMed=21498645; DOI=10.1128/jb.01188-10;
RA   Qu Y., Spain J.C.;
RT   "Molecular and biochemical characterization of the 5-nitroanthranilic acid
RT   degradation pathway in Bradyrhizobium sp. strain JS329.";
RL   J. Bacteriol. 193:3057-3063(2011).
CC   -!- FUNCTION: Catalyzes the deamination of 5-nitroanthranilate (5NAA) to 5-
CC       nitrosalicylate (5NSA), the first step in biodegradation of 5-
CC       nitroanthranilate. {ECO:0000269|PubMed:20081004,
CC       ECO:0000269|PubMed:21498645}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-nitroanthranilate + H(+) + H2O = 5-nitrosalicylate + NH4(+);
CC         Xref=Rhea:RHEA:28166, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:61267, ChEBI:CHEBI:61268; EC=3.5.99.8;
CC         Evidence={ECO:0000269|PubMed:21498645};
CC   -!- COFACTOR:
CC       Name=Co(2+); Xref=ChEBI:CHEBI:48828;
CC         Evidence={ECO:0000269|PubMed:21498645};
CC       Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC         Evidence={ECO:0000269|PubMed:21498645};
CC       Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC         Evidence={ECO:0000269|PubMed:21498645};
CC       Name=Fe(2+); Xref=ChEBI:CHEBI:29033;
CC         Evidence={ECO:0000269|PubMed:21498645};
CC       Name=Ni(2+); Xref=ChEBI:CHEBI:49786;
CC         Evidence={ECO:0000269|PubMed:21498645};
CC       Note=Divalent metal cations. Co(2+), Mn(2+), Zn(2+), Fe(2+) or Ni(2+).
CC       {ECO:0000269|PubMed:21498645};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=335.1 uM for 5-nitroanthranilate {ECO:0000269|PubMed:21498645};
CC         Vmax=181.5 nmol/min/mg enzyme {ECO:0000269|PubMed:21498645};
CC       pH dependence:
CC         Optimum pH is 7.2. {ECO:0000269|PubMed:21498645};
CC       Temperature dependence:
CC         Optimum temperature is degrees Celsius.
CC         {ECO:0000269|PubMed:21498645};
CC   -!- SIMILARITY: Belongs to the peptidase M20A family. {ECO:0000305}.
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DR   EMBL; GU188569; ADC93716.1; -; Genomic_DNA.
DR   PDB; 5K8M; X-ray; 2.75 A; A/B/C/D=1-425.
DR   PDB; 5K8N; X-ray; 3.23 A; A/B/C/D/E/F/G/H=1-425.
DR   PDB; 5K8O; X-ray; 2.89 A; A/B/C/D/E/F/G/H=1-425.
DR   PDB; 5K8P; X-ray; 2.20 A; A/B/C/D/E/F/G/H=1-425.
DR   PDBsum; 5K8M; -.
DR   PDBsum; 5K8N; -.
DR   PDBsum; 5K8O; -.
DR   PDBsum; 5K8P; -.
DR   AlphaFoldDB; D3WZ85; -.
DR   SMR; D3WZ85; -.
DR   PRIDE; D3WZ85; -.
DR   KEGG; ag:ADC93716; -.
DR   BioCyc; MetaCyc:MON-15902; -.
DR   BRENDA; 3.5.99.8; 930.
DR   GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   InterPro; IPR002933; Peptidase_M20.
DR   InterPro; IPR011650; Peptidase_M20_dimer.
DR   Pfam; PF07687; M20_dimer; 1.
DR   Pfam; PF01546; Peptidase_M20; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Hydrolase; Metal-binding.
FT   CHAIN           1..425
FT                   /note="5-nitroanthranilic acid aminohydrolase"
FT                   /id="PRO_0000418738"
FT   ACT_SITE        88
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        158
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   HELIX           6..15
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   HELIX           18..30
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   HELIX           39..51
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   STRAND          57..62
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   STRAND          65..72
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   STRAND          75..78
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   STRAND          81..87
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   HELIX           95..97
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   TURN            100..103
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   HELIX           105..108
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   STRAND          111..113
FT                   /evidence="ECO:0007829|PDB:5K8M"
FT   STRAND          114..118
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   HELIX           120..123
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   HELIX           126..141
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   STRAND          150..156
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   TURN            170..172
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   HELIX           175..177
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   HELIX           179..184
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   STRAND          190..194
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   STRAND          198..200
FT                   /evidence="ECO:0007829|PDB:5K8N"
FT   STRAND          208..217
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   HELIX           224..226
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   HELIX           233..235
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   HELIX           239..260
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   STRAND          263..265
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   STRAND          268..270
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   STRAND          274..283
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   STRAND          292..302
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   HELIX           308..321
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   STRAND          326..334
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   HELIX           343..357
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   HELIX           367..369
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   HELIX           375..380
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   STRAND          385..387
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   TURN            394..397
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   STRAND          400..402
FT                   /evidence="ECO:0007829|PDB:5K8P"
FT   HELIX           403..420
FT                   /evidence="ECO:0007829|PDB:5K8P"
SQ   SEQUENCE   425 AA;  46349 MW;  026B430C5B3C9276 CRC64;
     MAGSNDVAKV MKTLDGMREG LIQTAVELGS IEAPTGREGA AGDYVYEWMA RNGFGPERVG
     VFDDRFNVVG RLRGTGGGAS LSFNSHLDTI MAREDTARFA DANDRIYHEA WHEEGRIYGY
     SVVNCKGPMA CWLIAAKALK EAGAALKGDV VLTAVCGEID CEPVDEFQGH DYLAEDIGAR
     YAISHGAISD YALVAEATNF KPAWVEAGKV FLKVTVFAGP SRYTPYVPRP VAALDSPNAI
     VRMAKLVEAL EEWADNYEKR YTREYGGGTV VPKVAIGAIR GGVPYKIYRF PELCSIYMDI
     RLNPDTNPLV VQREVEAVVS KLGLKAEVKP FLFRRGYEAQ GIEPLQNALE VAHREVVGRP
     TERPGSPECS MWRDTNPYNE LGIPSLTYGC GGGAGGGNTY FLVDDMLKAA KVYAMTAMDL
     CNRTP
 
 
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