ID   NAAC_BRASZ              Reviewed;         248 AA.
AC   F8QQ74;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   21-SEP-2011, sequence version 1.
DT   03-AUG-2022, entry version 26.
DE   RecName: Full=Probable 2-oxo-3-(5-oxofuran-2-ylidene)propanoate lactonase;
DE            EC=3.1.1.91;
DE   AltName: Full=5-nitroanthranilic acid degradation protein C;
DE   AltName: Full=Lactone hydrolase NaaC;
GN   Name=naaC;
OS   Bradyrhizobium sp.
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Bradyrhizobiaceae; Bradyrhizobium; unclassified Bradyrhizobium.
OX   NCBI_TaxID=376;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=JS329;
RX   PubMed=20081004; DOI=10.1128/aem.02816-09;
RA   Qu Y., Spain J.C.;
RT   "Biodegradation of 5-Nitroanthranilic Acid by Bradyrhizobium sp. Strain
RT   JS329.";
RL   Appl. Environ. Microbiol. 76:1417-1422(2010).
RN   [2]
RP   FUNCTION.
RC   STRAIN=JS329;
RX   PubMed=21498645; DOI=10.1128/jb.01188-10;
RA   Qu Y., Spain J.C.;
RT   "Molecular and biochemical characterization of the 5-nitroanthranilic acid
RT   degradation pathway in Bradyrhizobium sp. strain JS329.";
RL   J. Bacteriol. 193:3057-3063(2011).
CC   -!- FUNCTION: Involved in the 5-nitroanthranilic acid (5NAA) degradation.
CC       Catalyzes the hydrolysis of the lactone to produce maleylpyruvate
CC       biodegradation of 5-nitroanthranilate (Probable).
CC       {ECO:0000305|PubMed:21498645}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-oxo-3-(5-oxofuran-2-ylidene)propanoate + H2O = 3-
CC         maleylpyruvate + H(+); Xref=Rhea:RHEA:33967, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16727, ChEBI:CHEBI:65081; EC=3.1.1.91;
CC   -!- SIMILARITY: Belongs to the dienelactone hydrolase family.
CC       {ECO:0000305}.
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DR   EMBL; GU188569; AEH76925.1; -; Genomic_DNA.
DR   AlphaFoldDB; F8QQ74; -.
DR   SMR; F8QQ74; -.
DR   KEGG; ag:AEH76925; -.
DR   BioCyc; MetaCyc:MON-17374; -.
DR   GO; GO:0102355; F:2-oxo-3-(5-oxofuran-2-ylidene)propanoate lactonase activity; IEA:UniProtKB-EC.
DR   GO; GO:0052689; F:carboxylic ester hydrolase activity; IDA:UniProtKB.
DR   GO; GO:0046573; F:lactonohydrolase activity; IDA:CACAO.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR002925; Dienelactn_hydro.
DR   Pfam; PF01738; DLH; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   3: Inferred from homology;
KW   Hydrolase.
FT   CHAIN           1..248
FT                   /note="Probable 2-oxo-3-(5-oxofuran-2-ylidene)propanoate
FT                   lactonase"
FT                   /id="PRO_0000418747"
FT   ACT_SITE        123
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        180
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        212
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   248 AA;  27660 MW;  FBE1325E4A0F7012 CRC64;
     MATETIAMDW VDIGTNGESR LAYLARPVVT GRLPAVIVMP AIHGINTYIK DVAIDLAKAG
     FVALLIDIHS PEQEPDLSNA EKIQIAVETL DDRKVLKDVD AAVRYLEQHA AVRADRLGIL
     GFCVGGTYAL LAARTPAIRV SVGFYGLLEY QSRTDNKPVS PLDSVAQFTA PILFHVGDKD
     PWIDSKMLAE FTKRMQQHQK SYELCIYRGA GHAFHEHFRD AYRPIAAQSA WNNTLIYLRW
     HLCGKRTV