NAAT1_ORYSJ
ID NAAT1_ORYSJ Reviewed; 494 AA.
AC A0A0P0VI36; A9CM06; C7IYK7; Q6K2Y8;
DT 08-MAY-2019, integrated into UniProtKB/Swiss-Prot.
DT 20-JAN-2016, sequence version 1.
DT 03-AUG-2022, entry version 41.
DE RecName: Full=Nicotianamine aminotransferase 1 {ECO:0000303|PubMed:15781441};
DE Short=OsNAAT1 {ECO:0000303|PubMed:15781441};
DE EC=2.6.1.80 {ECO:0000269|PubMed:18034312};
GN Name=NAAT1 {ECO:0000303|PubMed:15781441};
GN OrderedLocusNames=Os02g0306401 {ECO:0000312|EMBL:BAS78288.1},
GN LOC_Os02g20360 {ECO:0000305};
GN ORFNames=P0543C11.29 {ECO:0000312|EMBL:BAD23582.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Root;
RA Nishizawa N.K., Takahashi M., Suzuki M., Nakanishi H., Satoshi M.,
RA Yoshimura E.;
RT "Phytosiderophore secretion in zinc-deficient barley is independent of iron
RT deficiency.";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [5]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 35-494.
RX PubMed=16972867; DOI=10.1111/j.1365-313x.2006.02853.x;
RA Suzuki M., Takahashi M., Tsukamoto T., Watanabe S., Matsuhashi S.,
RA Yazaki J., Kishimoto N., Kikuchi S., Nakanishi H., Mori S., Nishizawa N.K.;
RT "Biosynthesis and secretion of mugineic acid family phytosiderophores in
RT zinc-deficient barley.";
RL Plant J. 48:85-97(2006).
RN [6]
RP INDUCTION BY IRON DEFICIENCY.
RX PubMed=15781441; DOI=10.1093/jxb/eri131;
RA Kobayashi T., Suzuki M., Inoue H., Itai R.N., Takahashi M., Nakanishi H.,
RA Mori S., Nishizawa N.K.;
RT "Expression of iron-acquisition-related genes in iron-deficient rice is co-
RT ordinately induced by partially conserved iron-deficiency-responsive
RT elements.";
RL J. Exp. Bot. 56:1305-1316(2005).
RN [7]
RP DEVELOPMENTAL STAGE.
RX PubMed=17333504; DOI=10.1007/s11103-007-9132-4;
RA Nozoye T., Inoue H., Takahashi M., Ishimaru Y., Nakanishi H., Mori S.,
RA Nishizawa N.K.;
RT "The expression of iron homeostasis-related genes during rice
RT germination.";
RL Plant Mol. Biol. 64:35-47(2007).
RN [8]
RP FUNCTION, CATALYTIC ACTIVITY, TISSUE SPECIFICITY, AND INDUCTION BY IRON
RP DEFICIENCY.
RX PubMed=18034312; DOI=10.1007/s11103-007-9262-8;
RA Inoue H., Takahashi M., Kobayashi T., Suzuki M., Nakanishi H., Mori S.,
RA Nishizawa N.K.;
RT "Identification and localisation of the rice nicotianamine aminotransferase
RT gene OsNAAT1 expression suggests the site of phytosiderophore synthesis in
RT rice.";
RL Plant Mol. Biol. 66:193-203(2008).
CC -!- FUNCTION: Involved in biosynthesis of mugineic acid family
CC phytosiderophores, which are ferric iron chelators produced in
CC graminaceous plants in response to iron deficiency.
CC {ECO:0000269|PubMed:18034312}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2-oxoglutarate + nicotianamine = 3''-deamino-3''-
CC oxonicotianamine + L-glutamate; Xref=Rhea:RHEA:22104,
CC ChEBI:CHEBI:16810, ChEBI:CHEBI:29985, ChEBI:CHEBI:58249,
CC ChEBI:CHEBI:58685; EC=2.6.1.80;
CC Evidence={ECO:0000269|PubMed:18034312};
CC -!- COFACTOR:
CC Name=pyridoxal 5'-phosphate; Xref=ChEBI:CHEBI:597326;
CC Evidence={ECO:0000250|UniProtKB:P17735};
CC -!- TISSUE SPECIFICITY: Expressed in companion and pericycle cells adjacent
CC to the protoxylem of roots (PubMed:18034312). Expressed in companion
CC cells of shoots (PubMed:18034312). {ECO:0000269|PubMed:18034312}.
CC -!- DEVELOPMENTAL STAGE: Expressed in the scutellum and leaf primordium of
CC fully mature seeds (PubMed:17333504). In germinating seeds, expressed
CC in the vascular bundle of the scutellum, the coleoptile, the bud scale,
CC the coleorhiza, and the base of the seminal root (PubMed:17333504).
CC {ECO:0000269|PubMed:17333504}.
CC -!- INDUCTION: Induced by iron deficiency in roots and leaves.
CC {ECO:0000269|PubMed:15781441, ECO:0000269|PubMed:18034312}.
CC -!- SIMILARITY: Belongs to the class-I pyridoxal-phosphate-dependent
CC aminotransferase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD23582.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAE86873.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAH91645.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB182275; BAF95202.1; -; mRNA.
DR EMBL; AP005743; BAD23582.1; ALT_INIT; Genomic_DNA.
DR EMBL; AP008208; BAH91645.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014958; BAS78288.1; -; Genomic_DNA.
DR EMBL; AB206814; BAE86873.1; ALT_INIT; mRNA.
DR RefSeq; XP_015627400.1; XM_015771914.1.
DR AlphaFoldDB; A0A0P0VI36; -.
DR SMR; A0A0P0VI36; -.
DR STRING; 4530.OS02T0306401-00; -.
DR PaxDb; A0A0P0VI36; -.
DR EnsemblPlants; Os02t0306401-00; Os02t0306401-00; Os02g0306401.
DR GeneID; 9267778; -.
DR Gramene; Os02t0306401-00; Os02t0306401-00; Os02g0306401.
DR KEGG; osa:9267778; -.
DR eggNOG; KOG0259; Eukaryota.
DR HOGENOM; CLU_017584_4_2_1; -.
DR OMA; GYCQAVL; -.
DR OrthoDB; 734452at2759; -.
DR BRENDA; 2.6.1.80; 4460.
DR PlantReactome; R-OSA-9025754; Mugineic acid biosynthesis.
DR Proteomes; UP000000763; Chromosome 2.
DR Proteomes; UP000059680; Chromosome 2.
DR GO; GO:0004838; F:L-tyrosine:2-oxoglutarate aminotransferase activity; IBA:GO_Central.
DR GO; GO:0033855; F:nicotianamine aminotransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0030170; F:pyridoxal phosphate binding; IEA:InterPro.
DR GO; GO:0009058; P:biosynthetic process; IEA:InterPro.
DR GO; GO:0006572; P:tyrosine catabolic process; IBA:GO_Central.
DR Gene3D; 3.40.640.10; -; 1.
DR Gene3D; 3.90.1150.10; -; 1.
DR InterPro; IPR004839; Aminotransferase_I/II.
DR InterPro; IPR015424; PyrdxlP-dep_Trfase.
DR InterPro; IPR015421; PyrdxlP-dep_Trfase_major.
DR InterPro; IPR015422; PyrdxlP-dep_Trfase_small.
DR InterPro; IPR005958; TyrNic_aminoTrfase.
DR Pfam; PF00155; Aminotran_1_2; 1.
DR PIRSF; PIRSF000517; Tyr_transaminase; 1.
DR SUPFAM; SSF53383; SSF53383; 1.
DR TIGRFAMs; TIGR01265; tyr_nico_aTase; 1.
PE 1: Evidence at protein level;
KW Aminotransferase; Pyridoxal phosphate; Reference proteome; Transferase.
FT CHAIN 1..494
FT /note="Nicotianamine aminotransferase 1"
FT /id="PRO_0000446970"
FT REGION 24..76
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 24..69
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 322
FT /note="N6-(pyridoxal phosphate)lysine"
FT /evidence="ECO:0000250|UniProtKB:P17735"
FT CONFLICT 38
FT /note="S -> F (in Ref. 1; BAF95202)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 494 AA; 53822 MW; 783C28C759FF1FAF CRC64;
MHASCCCAPP ESVSHTRRIS YKYSGTSYPT RTTTTSSSAP EFTNKKQSTA MAPTTAAAAA
SSNGGGESDG SSKEWRLTAP TRGGAMAAAG DKMSIRAVRY KISASVDDRG PRPVLPLAHG
DPSVFPEFRT AAEAEDAVAD ALRSGDFNCY PAGVGLPAAR RAVADHLSRD LPYKLSSDDI
FLTAGGTQAI EVVISILAQP GTNILLPRPG YPNYEARAAF NNLEVRHFDL IPEKGWEIDL
NSLESIADKN TTAIVIINPN NPCGNVYTYE HLSKVAEVAR KLGILVITDE VYGNLVFGSS
PFVPMGCFGH IVPILTIGSL SKRWIVPGWR LGWVAICDPK KTLQETKIAT LITNFLNVST
DPATFIQGAL PNILKNTKEE FFKRIIDLLT ETSDICYRGI KDIKCITCPH KPEGSMFVMV
KLNLYLLEGI HDDVDFCCQL AKEESVILCP GSVLGMKNWV RITFAIDSSS LLDGLERIKS
FCQRHKKKNP LNYI
