NAB_DROME
ID NAB_DROME Reviewed; 625 AA.
AC Q59E55; B5X541; E1JIC7;
DT 05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 21-SEP-2011, sequence version 3.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=NGFI-A-binding protein homolog;
DE Short=dNAB;
GN Name=nab; ORFNames=CG33545;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM B).
RC STRAIN=Berkeley; TISSUE=Larva, and Pupae;
RA Carlson J., Booth B., Frise E., Park S., Wan K., Yu C., Celniker S.;
RL Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, AND LACK OF INTERACTION
RP WITH KLU.
RX PubMed=12508226; DOI=10.1002/dvdy.10209;
RA Clements M., Duncan D., Milbrandt J.;
RT "Drosophila NAB (dNAB) is an orphan transcriptional co-repressor required
RT for correct CNS and eye development.";
RL Dev. Dyn. 226:67-81(2003).
RN [5]
RP FUNCTION, TISSUE SPECIFICITY, INDUCTION, AND INTERACTION WITH RN AND SQZ.
RX PubMed=17428824; DOI=10.1242/dev.003830;
RA Terriente Felix J., Magarinos M., Diaz-Benjumea F.J.;
RT "Nab controls the activity of the zinc-finger transcription factors Squeeze
RT and Rotund in Drosophila development.";
RL Development 134:1845-1852(2007).
RN [6]
RP FUNCTION.
RX PubMed=18571155; DOI=10.1016/j.ydbio.2008.04.028;
RA Terriente J., Perea D., Suzanne M., Diaz-Benjumea F.J.;
RT "The Drosophila gene zfh2 is required to establish proximal-distal domains
RT in the wing disc.";
RL Dev. Biol. 320:102-112(2008).
CC -!- FUNCTION: Transcriptional regulator that can both act as a coactivator
CC or a corepressor depending on the context. Lacks DNA-binding domains
CC and acts by associating with other transcription factors such as rotund
CC (rn) and squeeze (sqz). Acts as a coactivator of sqz and is required to
CC limit the number of neurons that express the LIM-homeodomain gene
CC apterous and to specify Tv neuronal fate. Acts as corepressor of rn in
CC wing development and is required to limit the expression of wingless
CC (wg) in the wing hinge, where wg plays a mitogenic role.
CC {ECO:0000269|PubMed:12508226, ECO:0000269|PubMed:17428824,
CC ECO:0000269|PubMed:18571155}.
CC -!- SUBUNIT: Interacts with sqz and rn. Able to bind mammalian EGR1 but not
CC Drosophila EGR-like protein klu. {ECO:0000269|PubMed:17428824}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=C;
CC IsoId=Q59E55-1; Sequence=Displayed;
CC Name=B;
CC IsoId=Q59E55-2; Sequence=VSP_041822;
CC -!- TISSUE SPECIFICITY: Expressed exclusively in a subset of neuroblasts in
CC the embryonic and larval central nervous system (CNS), as well as in
CC several larval imaginal disk tissues. Co-expressed with sqz in a subset
CC of neurons in the embryonic ventral nerve cord and with rn in a
CC circular domain of the distal-most area of the wing disk.
CC {ECO:0000269|PubMed:12508226, ECO:0000269|PubMed:17428824}.
CC -!- INDUCTION: Regulated by vestigial (vg) in the wing.
CC {ECO:0000269|PubMed:17428824}.
CC -!- DOMAIN: The NAB conserved domain 2 (NCD2) is necessary for
CC transcriptional repression. {ECO:0000250}.
CC -!- DISRUPTION PHENOTYPE: Larval locomotion defects and early larval
CC lethality (L1-L2). In the CNS, axon outgrowth/guidance and glial
CC development appear normal; however, a subset of eve(+) neurons forms in
CC reduced numbers. {ECO:0000269|PubMed:12508226}.
CC -!- SIMILARITY: Belongs to the NAB family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ACI46548.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE014296; AAX52730.3; -; Genomic_DNA.
DR EMBL; AE014296; ACZ94616.1; -; Genomic_DNA.
DR EMBL; BT046160; ACI46548.1; ALT_INIT; mRNA.
DR RefSeq; NP_001014557.3; NM_001014557.2. [Q59E55-1]
DR RefSeq; NP_001163344.1; NM_001169873.1. [Q59E55-2]
DR AlphaFoldDB; Q59E55; -.
DR SMR; Q59E55; -.
DR BioGRID; 77955; 5.
DR IntAct; Q59E55; 2.
DR STRING; 7227.FBpp0291243; -.
DR PaxDb; Q59E55; -.
DR EnsemblMetazoa; FBtr0302032; FBpp0291242; FBgn0259986. [Q59E55-2]
DR EnsemblMetazoa; FBtr0302033; FBpp0291243; FBgn0259986. [Q59E55-1]
DR GeneID; 3346237; -.
DR KEGG; dme:Dmel_CG33545; -.
DR UCSC; CG33545-RA; d. melanogaster. [Q59E55-1]
DR CTD; 3346237; -.
DR FlyBase; FBgn0259986; nab.
DR VEuPathDB; VectorBase:FBgn0259986; -.
DR eggNOG; KOG3835; Eukaryota.
DR GeneTree; ENSGT00390000006330; -.
DR InParanoid; Q59E55; -.
DR OMA; TCDEHEF; -.
DR PhylomeDB; Q59E55; -.
DR BioGRID-ORCS; 3346237; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 3346237; -.
DR PRO; PR:Q59E55; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0259986; Expressed in wing disc and 5 other tissues.
DR ExpressionAtlas; Q59E55; baseline and differential.
DR Genevisible; Q59E55; DM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003712; F:transcription coregulator activity; IMP:FlyBase.
DR GO; GO:0007626; P:locomotory behavior; IMP:FlyBase.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0014019; P:neuroblast development; IMP:FlyBase.
DR GO; GO:0048666; P:neuron development; IMP:FlyBase.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IBA:GO_Central.
DR GO; GO:0035222; P:wing disc pattern formation; IMP:FlyBase.
DR Gene3D; 1.20.120.2010; -; 1.
DR InterPro; IPR006989; NAB_co-repressor_dom.
DR InterPro; IPR039040; NAB_fam.
DR InterPro; IPR006988; Nab_N.
DR InterPro; IPR038398; NCD2_sf.
DR PANTHER; PTHR12623; PTHR12623; 1.
DR Pfam; PF04904; NCD1; 1.
DR Pfam; PF04905; NCD2; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Nucleus; Reference proteome; Repressor;
KW Transcription; Transcription regulation.
FT CHAIN 1..625
FT /note="NGFI-A-binding protein homolog"
FT /id="PRO_0000372658"
FT REGION 1..47
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 75..111
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 111..189
FT /note="NCD1"
FT REGION 220..247
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 276..305
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 340..427
FT /note="NCD2"
FT REGION 447..480
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 532..578
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 233..247
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 283..305
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 532..547
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 558..578
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 546..625
FT /note="TPCNGMEGERSPVSRQMETSSPPRESVDLSGTSSGAALSGVQVISAAGDNII
FT AVANPALALSPALNEVLALKRSAASPET -> VSMCWSSTRAFRYPGKAEE (in
FT isoform B)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_041822"
FT CONFLICT 194
FT /note="M -> I (in Ref. 3; ACI46548)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 625 AA; 66488 MW; 01749623C8CA3045 CRC64;
MEASSNPTTP TTAVATTTST SSPSPAASTS SKGHSQSATA SASASATQSQ LLTTSLEMPK
TEDLYNLSVA SGLSEGQRSL SGAPSASSSP IMSPQGKIFG RNANGTMITT SRPGNEAEVQ
LYRVLQRASL LAYYDTLLEM GGDDVQQLYD AGEEEFLEIM ALVGMASKPL HVRRLQKALH
EWANNPGLFQ GPMMPHLGLC ETPPKPALIF NPDTTPALPR QKFPSFNPSG SSFMPSPVPP
APLPASASVP APTVPLATQI SCPSAPSVPL PLVLPPNPLT SSPHPSANSN LPANTAHQVS
SSSPQLTPVL TEAQIQRITM CADKIGRQLP QREPRAQTTR KRTTRELEQV IAMGEQDPRR
MDEIRKYSAI YGRFDCKRRP EKPLTLHEVC VNEAAAQLCR NPQTIWLLTR RDELFPLARQ
IVKDAGFGHS ASIARYGGLL TQLPSQGAGL GGGRAPGDTD CESSDAAVPS KRQRLSSTEA
AQLPLDLNRE AVEDSRYNLF AMYQKFAKPP FDLSEIAKFS VSKAADYEDN DSRFSFSNSS
SPTMPTPCNG MEGERSPVSR QMETSSPPRE SVDLSGTSSG AALSGVQVIS AAGDNIIAVA
NPALALSPAL NEVLALKRSA ASPET
