ID   NAC12_ARATH             Reviewed;         358 AA.
AC   Q9LPI7;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=NAC domain-containing protein 12 {ECO:0000303|PubMed:15029955};
DE            Short=ANAC012 {ECO:0000303|PubMed:15029955};
DE   AltName: Full=Protein NAC SECONDARY WALL THICKENING PROMOTING 3 {ECO:0000303|PubMed:17237351};
DE   AltName: Full=Protein SECONDARY WALL-ASSOCIATED NAC DOMAIN PROTEIN 1 {ECO:0000303|PubMed:17114348};
GN   Name=NAC012 {ECO:0000312|EMBL:AEE31527.1};
GN   Synonyms=NST3 {ECO:0000303|PubMed:17237351},
GN   SND1 {ECO:0000303|PubMed:17114348}; OrderedLocusNames=At1g32770;
GN   ORFNames=F6N18.15;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=17114348; DOI=10.1105/tpc.106.047399;
RA   Zhong R., Demura T., Ye Z.-H.;
RT   "SND1, a NAC domain transcription factor, is a key regulator of secondary
RT   wall synthesis in fibers of Arabidopsis.";
RL   Plant Cell 18:3158-3170(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [4]
RP   GENE FAMILY, AND NOMENCLATURE.
RX   PubMed=15029955; DOI=10.1093/dnares/10.6.239;
RA   Ooka H., Satoh K., Doi K., Nagata T., Otomo Y., Murakami K., Matsubara K.,
RA   Osato N., Kawai J., Carninci P., Hayashizaki Y., Suzuki K., Kojima K.,
RA   Takahara Y., Yamamoto K., Kikuchi S.;
RT   "Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis
RT   thaliana.";
RL   DNA Res. 10:239-247(2003).
RN   [5]
RP   TISSUE SPECIFICITY.
RX   PubMed=15923329; DOI=10.1104/pp.105.060202;
RA   Zhao C., Craig J.C., Petzold H.E., Dickerman A.W., Beers E.P.;
RT   "The xylem and phloem transcriptomes from secondary tissues of the
RT   Arabidopsis root-hypocotyl.";
RL   Plant Physiol. 138:803-818(2005).
RN   [6]
RP   FUNCTION.
RX   PubMed=17333250; DOI=10.1007/s00425-007-0498-y;
RA   Zhong R., Richardson E.A., Ye Z.-H.;
RT   "Two NAC domain transcription factors, SND1 and NST1, function redundantly
RT   in regulation of secondary wall synthesis in fibers of Arabidopsis.";
RL   Planta 225:1603-1611(2007).
RN   [7]
RP   FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=17237351; DOI=10.1105/tpc.106.047043;
RA   Mitsuda N., Iwase A., Yamamoto H., Yoshida M., Seki M., Shinozaki K.,
RA   Ohme-Takagi M.;
RT   "NAC transcription factors, NST1 and NST3, are key regulators of the
RT   formation of secondary walls in woody tissues of Arabidopsis.";
RL   Plant Cell 19:270-280(2007).
RN   [8]
RP   FUNCTION.
RX   PubMed=17890373; DOI=10.1105/tpc.107.053678;
RA   Zhong R., Richardson E.A., Ye Z.-H.;
RT   "The MYB46 transcription factor is a direct target of SND1 and regulates
RT   secondary wall biosynthesis in Arabidopsis.";
RL   Plant Cell 19:2776-2792(2007).
RN   [9]
RP   FUNCTION, AND TISSUE SPECIFICITY.
RX   PubMed=17565617; DOI=10.1111/j.1365-313x.2007.03109.x;
RA   Ko J.-H., Yang S.H., Park A.H., Lerouxel O., Han K.-H.;
RT   "ANAC012, a member of the plant-specific NAC transcription factor family,
RT   negatively regulates xylary fiber development in Arabidopsis thaliana.";
RL   Plant J. 50:1035-1048(2007).
RN   [10]
RP   SUBCELLULAR LOCATION.
RX   PubMed=18952777; DOI=10.1105/tpc.108.061325;
RA   Zhong R., Lee C., Zhou J., McCarthy R.L., Ye Z.H.;
RT   "A battery of transcription factors involved in the regulation of secondary
RT   cell wall biosynthesis in Arabidopsis.";
RL   Plant Cell 20:2763-2782(2008).
CC   -!- FUNCTION: Transcriptional activator of genes involved in biosynthesis
CC       of secondary walls. Together with NST1, required for the secondary cell
CC       wall thickening and lignification of sclerenchymatous fibers and
CC       secondary xylem vessels (tracheary elements). Seems to repress the
CC       secondary cell wall thickening of xylary fibers. May also regulate the
CC       secondary cell wall lignification of other tissues. Binds to and
CC       activates the promoter of MYB46. {ECO:0000269|PubMed:17114348,
CC       ECO:0000269|PubMed:17237351, ECO:0000269|PubMed:17333250,
CC       ECO:0000269|PubMed:17565617, ECO:0000269|PubMed:17890373}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00353,
CC       ECO:0000269|PubMed:18952777}.
CC   -!- TISSUE SPECIFICITY: Stems and roots, specifically in interfascicular
CC       fibers (sclerenchyma), cells differentiating into vascular vessels
CC       (cambium), and xylem. {ECO:0000269|PubMed:15923329,
CC       ECO:0000269|PubMed:17114348, ECO:0000269|PubMed:17237351,
CC       ECO:0000269|PubMed:17565617}.
CC   -!- DOMAIN: The NAC domain includes a DNA-binding domain and a dimerization
CC       domain. {ECO:0000255|PROSITE-ProRule:PRU00353}.
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DR   EMBL; EF101892; ABL67723.1; -; mRNA.
DR   EMBL; AC017118; AAF25976.1; -; Genomic_DNA.
DR   EMBL; CP002684; AEE31527.1; -; Genomic_DNA.
DR   PIR; E86452; E86452.
DR   RefSeq; NP_174554.1; NM_103011.2.
DR   AlphaFoldDB; Q9LPI7; -.
DR   SMR; Q9LPI7; -.
DR   BioGRID; 25405; 20.
DR   IntAct; Q9LPI7; 20.
DR   STRING; 3702.AT1G32770.1; -.
DR   PaxDb; Q9LPI7; -.
DR   PRIDE; Q9LPI7; -.
DR   EnsemblPlants; AT1G32770.1; AT1G32770.1; AT1G32770.
DR   GeneID; 840171; -.
DR   Gramene; AT1G32770.1; AT1G32770.1; AT1G32770.
DR   KEGG; ath:AT1G32770; -.
DR   Araport; AT1G32770; -.
DR   TAIR; locus:2035604; AT1G32770.
DR   eggNOG; ENOG502QSBA; Eukaryota.
DR   HOGENOM; CLU_035664_1_2_1; -.
DR   InParanoid; Q9LPI7; -.
DR   OMA; DIAHSMH; -.
DR   OrthoDB; 1109365at2759; -.
DR   PhylomeDB; Q9LPI7; -.
DR   PRO; PR:Q9LPI7; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q9LPI7; baseline and differential.
DR   Genevisible; Q9LPI7; AT.
DR   GO; GO:0005634; C:nucleus; IDA:TAIR.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IDA:TAIR.
DR   GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR   GO; GO:0010047; P:fruit dehiscence; IMP:TAIR.
DR   GO; GO:0009834; P:plant-type secondary cell wall biogenesis; IMP:TAIR.
DR   GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:TAIR.
DR   Gene3D; 2.170.150.80; -; 1.
DR   InterPro; IPR003441; NAC-dom.
DR   InterPro; IPR036093; NAC_dom_sf.
DR   Pfam; PF02365; NAM; 1.
DR   SUPFAM; SSF101941; SSF101941; 1.
DR   PROSITE; PS51005; NAC; 1.
PE   2: Evidence at transcript level;
KW   Activator; DNA-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..358
FT                   /note="NAC domain-containing protein 12"
FT                   /id="PRO_0000313829"
FT   DOMAIN          16..177
FT                   /note="NAC"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT   DNA_BIND        116..183
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
SQ   SEQUENCE   358 AA;  41520 MW;  3C6F27668B0140F5 CRC64;
     MADNKVNLSI NGQSKVPPGF RFHPTEEELL HYYLRKKVNS QKIDLDVIRE VDLNKLEPWD
     IQEECRIGST PQNDWYFFSH KDKKYPTGTR TNRATVAGFW KATGRDKIIC SCVRRIGLRK
     TLVFYKGRAP HGQKSDWIMH EYRLDDTPMS NGYADVVTED PMSYNEEGWV VCRVFRKKNY
     QKIDDCPKIT LSSLPDDTEE EKGPTFHNTQ NVTGLDHVLL YMDRTGSNIC MPESQTTTQH
     QDDVLFMQLP SLETPKSESP VDQSFLTPSK LDFSPVQEKI TERPVCSNWA SLDRLVAWQL
     NNGHHNPCHR KSFDEEEENG DTMMQRWDLH WNNDDNVDLW SSFTESSSSL DPLLHLSV