NAC20_ORYSI
ID NAC20_ORYSI Reviewed; 320 AA.
AC A2WJP3; A0A1L3MZD9;
DT 02-JUN-2021, integrated into UniProtKB/Swiss-Prot.
DT 20-MAR-2007, sequence version 1.
DT 25-MAY-2022, entry version 67.
DE RecName: Full=NAC domain-containing protein 20 {ECO:0000303|PubMed:27872632};
DE Short=ONAC020 {ECO:0000303|PubMed:27872632};
DE Short=OsNAC20 {ECO:0000303|PubMed:32989010};
GN Name=NAC20 {ECO:0000303|PubMed:27872632}; Synonyms=DLN1 {ECO:0000305};
GN ORFNames=OsI_00041 {ECO:0000312|EMBL:EAY72189.1};
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH NAC26, SUBCELLULAR
RP LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=27872632; DOI=10.3389/fpls.2016.01638;
RA Mathew I.E., Das S., Mahto A., Agarwal P.;
RT "Three rice NAC transcription factors heteromerize and are associated with
RT seed size.";
RL Front. Plant Sci. 7:1638-1638(2016).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [3]
RP INTERACTION WITH NAC26, AND SUBCELLULAR LOCATION.
RX PubMed=32989010; DOI=10.1104/pp.20.00984;
RA Wang J., Chen Z., Zhang Q., Meng S., Wei C.;
RT "The NAC transcription factors OsNAC20 and OsNAC26 regulate starch and
RT storage protein synthesis.";
RL Plant Physiol. 184:1775-1791(2020).
CC -!- FUNCTION: Transcription factor that acts redundantly with NAC26 to
CC regulate the expression of genes involved in the biosynthesis of starch
CC and storage proteins in grain. Directly binds to the promoters of
CC starch synthase 1 (SS1), pullulanase (PUL), glutelin A1 (GLUA1),
CC glutelins B4 and B5 (GLUB4 and GLUB5), alpha-globulin and 16 kDa
CC prolamin, and activates their expression (By similarity). Possesses
CC transactivation activity in yeast (PubMed:27872632).
CC {ECO:0000250|UniProtKB:Q9FTY0, ECO:0000269|PubMed:27872632}.
CC -!- SUBUNIT: Forms homodimers (PubMed:32989010). Forms heterodimers with
CC NAC26 (PubMed:27872632, PubMed:32989010). {ECO:0000269|PubMed:27872632,
CC ECO:0000269|PubMed:32989010}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00353,
CC ECO:0000269|PubMed:32989010}. Endoplasmic reticulum
CC {ECO:0000269|PubMed:27872632}.
CC -!- TISSUE SPECIFICITY: Expressed in developing seeds.
CC {ECO:0000269|PubMed:27872632}.
CC -!- DOMAIN: The NAC domain includes a DNA binding domain and a dimerization
CC domain. {ECO:0000255|PROSITE-ProRule:PRU00353}.
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DR EMBL; KX953272; APH07720.1; -; mRNA.
DR EMBL; KX953277; APH07725.1; -; mRNA.
DR EMBL; KX953276; APH07724.1; -; mRNA.
DR EMBL; CM000126; EAY72189.1; -; Genomic_DNA.
DR AlphaFoldDB; A2WJP3; -.
DR SMR; A2WJP3; -.
DR STRING; 39946.A2WJP3; -.
DR EnsemblPlants; BGIOSGA002597-TA; BGIOSGA002597-PA; BGIOSGA002597.
DR Gramene; BGIOSGA002597-TA; BGIOSGA002597-PA; BGIOSGA002597.
DR HOGENOM; CLU_035664_6_0_1; -.
DR OMA; LMAPPSY; -.
DR Proteomes; UP000007015; Chromosome 1.
DR GO; GO:0005783; C:endoplasmic reticulum; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0042803; F:protein homodimerization activity; IDA:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:EnsemblPlants.
DR GO; GO:2000014; P:regulation of endosperm development; IEA:EnsemblPlants.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:UniProtKB.
DR Gene3D; 2.170.150.80; -; 1.
DR InterPro; IPR003441; NAC-dom.
DR InterPro; IPR036093; NAC_dom_sf.
DR Pfam; PF02365; NAM; 1.
DR SUPFAM; SSF101941; SSF101941; 1.
DR PROSITE; PS51005; NAC; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Endoplasmic reticulum; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..320
FT /note="NAC domain-containing protein 20"
FT /id="PRO_0000452671"
FT DOMAIN 14..170
FT /note="NAC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT DNA_BIND 114..176
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT CONFLICT 239..243
FT /note="SYSTL -> PPP (in Ref. 1; APH07720/APH07724/
FT APH07725)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 320 AA; 35713 MW; A9A6D45FD37F1D43 CRC64;
MGEQQQQVER QPDLPPGFRF HPTDEEIITF YLAPKVVDSR GFCVAAIGEV DLNKCEPWDL
PGKAKMNGEK EWYFYCQKDR KYPTGMRTNR ATEAGYWKAT GKDKEIFRNH HMLIGMKKTL
VFYKGRAPKG DKTNWVMHEY RLADASPPPP PSSAEPPRQD DWAVCRIFHK SSGIKKPVPV
APHQVPAAAN YQQQQQMAMA SAGIIQVPMQ MQMPSMSDQL QMLDDFSTTA SLSLMAPPSY
STLPAGFPLQ INSGAHPQQF VGNPSMYYHQ QQQMDMAGGG FVVSEPSSLV VSPQDAADQN
NNAADISSMA CTMDAAIWKY
