NAC29_ARATH
ID NAC29_ARATH Reviewed; 268 AA.
AC O49255; Q8LD99;
DT 19-JUL-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 25-MAY-2022, entry version 134.
DE RecName: Full=NAC transcription factor 29;
DE Short=AtNAC029;
DE AltName: Full=Protein NAC-LIKE, ACTIVATED BY AP3/PI;
DE Short=AtNAP;
GN Name=NAC029; Synonyms=ANAC029, NAC29, NAP; OrderedLocusNames=At1g69490;
GN ORFNames=F10D13.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], DEVELOPMENTAL STAGE, AND INDUCTION.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=9489703; DOI=10.1016/s0092-8674(00)80902-2;
RA Sablowski R.W.M., Meyerowitz E.M.;
RT "A homolog of NO APICAL MERISTEM is an immediate target of the floral
RT homeotic genes APETALA3/PISTILLATA.";
RL Cell 92:93-103(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15029955; DOI=10.1093/dnares/10.6.239;
RA Ooka H., Satoh K., Doi K., Nagata T., Otomo Y., Murakami K., Matsubara K.,
RA Osato N., Kawai J., Carninci P., Hayashizaki Y., Suzuki K., Kojima K.,
RA Takahara Y., Yamamoto K., Kikuchi S.;
RT "Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis
RT thaliana.";
RL DNA Res. 10:239-247(2003).
RN [7]
RP FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, SUBCELLULAR LOCATION,
RP AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=16640597; DOI=10.1111/j.1365-313x.2006.02723.x;
RA Guo Y., Gan S.;
RT "AtNAP, a NAC family transcription factor, has an important role in leaf
RT senescence.";
RL Plant J. 46:601-612(2006).
RN [8]
RP FUNCTION.
RX PubMed=18849494; DOI=10.1105/tpc.108.060160;
RA Kunieda T., Mitsuda N., Ohme-Takagi M., Takeda S., Aida M., Tasaka M.,
RA Kondo M., Nishimura M., Hara-Nishimura I.;
RT "NAC family proteins NARS1/NAC2 and NARS2/NAM in the outer integument
RT regulate embryogenesis in Arabidopsis.";
RL Plant Cell 20:2631-2642(2008).
RN [9]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22184656; DOI=10.1104/pp.111.190876;
RA Zhang K., Gan S.S.;
RT "An abscisic acid-AtNAP transcription factor-SAG113 protein phosphatase 2C
RT regulatory chain for controlling dehydration in senescing Arabidopsis
RT leaves.";
RL Plant Physiol. 158:961-969(2012).
RN [10]
RP INDUCTION BY SENESCENCE.
RX PubMed=24659488; DOI=10.1093/jxb/eru112;
RA Kim H.J., Hong S.H., Kim Y.W., Lee I.H., Jun J.H., Phee B.K., Rupak T.,
RA Jeong H., Lee Y., Hong B.S., Nam H.G., Woo H.R., Lim P.O.;
RT "Gene regulatory cascade of senescence-associated NAC transcription factors
RT activated by ETHYLENE-INSENSITIVE2-mediated leaf senescence signalling in
RT Arabidopsis.";
RL J. Exp. Bot. 65:4023-4036(2014).
RN [11]
RP FUNCTION, INDUCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=25516602; DOI=10.1105/tpc.114.133769;
RA Yang J., Worley E., Udvardi M.;
RT "A NAP-AAO3 regulatory module promotes chlorophyll degradation via ABA
RT biosynthesis in Arabidopsis leaves.";
RL Plant Cell 26:4862-4874(2014).
CC -!- FUNCTION: Transcription activator that binds to, and transactivates the
CC promoter of the abscisic aldehyde oxidase AAO3. Promotes chlorophyll
CC degradation in leaves by enhancing transcription of AAO3, which leads
CC to increased levels of the senescence-inducing hormone abscisic acid
CC (ABA) (PubMed:25516602). Involved in the control of dehydration in
CC senescing leaves. Binds to the DNA sequence 5'-CACGTAAGT-3' of SAG113
CC promoter. SAG113 acts as negative regulator of ABA signaling for
CC stomatal closure in leaves, and controls water loss during leaf
CC senescence (PubMed:22184656). Transcription factor of the NAC family
CC involved in senescence. May function in the transition between active
CC cell division and cell expansion (PubMed:16640597). Required for normal
CC seed development and morphology (PubMed:18849494).
CC {ECO:0000269|PubMed:16640597, ECO:0000269|PubMed:18849494,
CC ECO:0000269|PubMed:22184656, ECO:0000269|PubMed:25516602}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00353,
CC ECO:0000269|PubMed:16640597}.
CC -!- TISSUE SPECIFICITY: Expressed in senescing leaves, petals and sepals.
CC {ECO:0000269|PubMed:16640597}.
CC -!- DEVELOPMENTAL STAGE: Expressed at the base of the inflorescence
CC meristem and at late stages of development in petals and stamens. Up-
CC regulated during leaf senescence. {ECO:0000269|PubMed:16640597,
CC ECO:0000269|PubMed:9489703}.
CC -!- INDUCTION: Induced by the heterodimer APETALA3 (AP3)/PISTILLATA (PI)
CC (PubMed:9489703). Induced by senescence (PubMed:22184656,
CC PubMed:24659488, PubMed:25516602). Induced by abscisic acid (ABA)
CC (PubMed:22184656, PubMed:25516602). Induced by ethylene
CC (PubMed:25516602). {ECO:0000269|PubMed:22184656,
CC ECO:0000269|PubMed:24659488, ECO:0000269|PubMed:25516602,
CC ECO:0000269|PubMed:9489703}.
CC -!- DOMAIN: The NAC domain includes a DNA-binding domain and a dimerization
CC domain. {ECO:0000255|PROSITE-ProRule:PRU00353}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype, but delayed leaf senescence
CC (PubMed:16640597, PubMed:22184656). No visible phenotype under normal
CC growth conditions, but mutant leaves show a stay-green phenotype and
CC deficiency in chlorophyll degradation during extended darkness.
CC {ECO:0000269|PubMed:16640597, ECO:0000269|PubMed:22184656}.
CC -!- MISCELLANEOUS: Plants silencing NAC029 produce abnormally shaped seeds.
CC {ECO:0000269|PubMed:18849494}.
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DR EMBL; AJ222713; CAA10955.1; -; Genomic_DNA.
DR EMBL; AY051015; AAK93692.1; -; mRNA.
DR EMBL; AC073178; AAG60108.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE34932.1; -; Genomic_DNA.
DR EMBL; AF360201; AAK25911.1; -; mRNA.
DR EMBL; AY086124; AAM63330.1; -; mRNA.
DR PIR; T52343; T52343.
DR RefSeq; NP_564966.1; NM_105616.4.
DR AlphaFoldDB; O49255; -.
DR SMR; O49255; -.
DR BioGRID; 28503; 6.
DR IntAct; O49255; 2.
DR STRING; 3702.AT1G69490.1; -.
DR iPTMnet; O49255; -.
DR PaxDb; O49255; -.
DR PRIDE; O49255; -.
DR ProteomicsDB; 251034; -.
DR EnsemblPlants; AT1G69490.1; AT1G69490.1; AT1G69490.
DR GeneID; 843282; -.
DR Gramene; AT1G69490.1; AT1G69490.1; AT1G69490.
DR KEGG; ath:AT1G69490; -.
DR Araport; AT1G69490; -.
DR TAIR; locus:2007166; AT1G69490.
DR eggNOG; ENOG502QSIY; Eukaryota.
DR HOGENOM; CLU_035664_8_2_1; -.
DR InParanoid; O49255; -.
DR OMA; NRQIGSM; -.
DR OrthoDB; 1011164at2759; -.
DR PhylomeDB; O49255; -.
DR PRO; PR:O49255; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; O49255; baseline and differential.
DR Genevisible; O49255; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:UniProtKB.
DR GO; GO:0009908; P:flower development; IMP:TAIR.
DR GO; GO:0009835; P:fruit ripening; IMP:CACAO.
DR GO; GO:0010150; P:leaf senescence; IMP:TAIR.
DR GO; GO:0009825; P:multidimensional cell growth; IMP:TAIR.
DR Gene3D; 2.170.150.80; -; 1.
DR InterPro; IPR003441; NAC-dom.
DR InterPro; IPR036093; NAC_dom_sf.
DR Pfam; PF02365; NAM; 1.
DR SUPFAM; SSF101941; SSF101941; 1.
DR PROSITE; PS51005; NAC; 1.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..268
FT /note="NAC transcription factor 29"
FT /id="PRO_0000132311"
FT DOMAIN 9..161
FT /note="NAC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT DNA_BIND 106..167
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT CONFLICT 145
FT /note="N -> S (in Ref. 5; AAM63330)"
FT /evidence="ECO:0000305"
FT CONFLICT 188
FT /note="V -> I (in Ref. 5; AAM63330)"
FT /evidence="ECO:0000305"
FT CONFLICT 195..196
FT /note="TE -> ND (in Ref. 5; AAM63330)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 268 AA; 31426 MW; B2CF930077E51E6E CRC64;
MEVTSQSTLP PGFRFHPTDE ELIVYYLRNQ TMSKPCPVSI IPEVDIYKFD PWQLPEKTEF
GENEWYFFSP RERKYPNGVR PNRAAVSGYW KATGTDKAIH SGSSNVGVKK ALVFYKGRPP
KGIKTDWIMH EYRLHDSRKA STKRNGSMRL DEWVLCRIYK KRGASKLLNE QEGFMDEVLM
EDETKVVVNE AERRTEEEIM MMTSMKLPRT CSLAHLLEMD YMGPVSHIDN FSQFDHLHQP
DSESSWFGDL QFNQDEILNH HRQAMFKF
