ID   NAC2_CHLRE              Reviewed;        1385 AA.
AC   Q9LEM8;
DT   10-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=PsbD mRNA maturation factor Nac2, chloroplastic;
DE   Flags: Precursor;
GN   Name=NAC2;
OS   Chlamydomonas reinhardtii (Chlamydomonas smithii).
OC   Eukaryota; Viridiplantae; Chlorophyta; core chlorophytes; Chlorophyceae;
OC   CS clade; Chlamydomonadales; Chlamydomonadaceae; Chlamydomonas.
OX   NCBI_TaxID=3055;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND MUTAGENESIS OF ALA-1038.
RC   STRAIN=137c / CC-125;
RX   PubMed=10880449; DOI=10.1093/emboj/19.13.3366;
RA   Boudreau E., Nickelsen J., Lemaire S.D., Ossenbuehl F., Rochaix J.-D.;
RT   "The Nac2 gene of Chlamydomonas encodes a chloroplast TPR-like protein
RT   involved in psbD mRNA stability.";
RL   EMBO J. 19:3366-3376(2000).
CC   -!- FUNCTION: Involved, directly or indirectly, in the processing of the
CC       chloroplast encoded psbD mRNA to its mature form, acting via the 5'-UTR
CC       of the psbD mRNA. The last 588 amino acids of the protein are
CC       sufficient to confer stability on the transcript in vivo.
CC   -!- SUBUNIT: Part of 2 complexes of about 600 and less than 2000 kDa, both
CC       of which also contain non-polysomal RNA.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast stroma.
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DR   EMBL; AJ271460; CAB96529.1; -; mRNA.
DR   AlphaFoldDB; Q9LEM8; -.
DR   STRING; 3055.EDP08046; -.
DR   GO; GO:0009570; C:chloroplast stroma; IEA:UniProtKB-SubCell.
DR   GO; GO:0003729; F:mRNA binding; IEA:InterPro.
DR   GO; GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
DR   Gene3D; 1.25.40.10; -; 2.
DR   InterPro; IPR003107; HAT.
DR   InterPro; IPR044624; Mbb1-like.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   InterPro; IPR019734; TPR_repeat.
DR   PANTHER; PTHR44917; PTHR44917; 1.
DR   SMART; SM00386; HAT; 5.
DR   SUPFAM; SSF48452; SSF48452; 2.
DR   PROSITE; PS50005; TPR; 2.
DR   PROSITE; PS50293; TPR_REGION; 1.
PE   1: Evidence at protein level;
KW   Chloroplast; mRNA processing; Plastid; Repeat; TPR repeat; Transit peptide.
FT   TRANSIT         1..45
FT                   /note="Chloroplast"
FT                   /evidence="ECO:0000255"
FT   CHAIN           46..1385
FT                   /note="PsbD mRNA maturation factor Nac2, chloroplastic"
FT                   /id="PRO_0000035684"
FT   REPEAT          851..884
FT                   /note="TPR 1"
FT   REPEAT          951..984
FT                   /note="TPR 2"
FT   REPEAT          985..1018
FT                   /note="TPR 3"
FT   REPEAT          1019..1052
FT                   /note="TPR 4"
FT   REPEAT          1053..1086
FT                   /note="TPR 5"
FT   REPEAT          1091..1124
FT                   /note="TPR 6"
FT   REPEAT          1125..1158
FT                   /note="TPR 7"
FT   REPEAT          1160..1193
FT                   /note="TPR 8"
FT   REPEAT          1205..1238
FT                   /note="TPR 9"
FT   REGION          132..157
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          263..282
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          304..360
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          387..502
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          546..568
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          726..756
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          840..899
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1237..1257
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1333..1385
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        311..331
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        387..407
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        550..568
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        726..748
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         1038
FT                   /note="A->E: Loss of mutant complementation; protein
FT                   accumulation decreases and the protein forms high molecular
FT                   weight aggregates."
FT                   /evidence="ECO:0000269|PubMed:10880449"
SQ   SEQUENCE   1385 AA;  144060 MW;  4DB548A850B405FF CRC64;
     MGALPCPAHI EHHQGLSSFG TRRVLRQSVA CGAHRSRRRS LWAGASPGAP KYDEGALSCS
     FQLGSASLHV SEVRGSRHAT PRAPPTGGCI ELPKTLALTE EQDQRAARAT SQLLNAISRC
     AAGSPLARLL TGPHGAASAT GAGSHSSSAG APTPTPRPDI AAAAALLPSL TWRWVSVADL
     EGGWVDRGVA HLLREALLVQ KAASDVNASV SELVGWCMDN CSAPAAALAA LWGQQQGAAL
     PARSRSFYGL LLQRLDGGGA SSAVAAAAGR QPHEHQQERS SAEAYGLSPA LVFCSSVART
     ALSTSSRRGG RSSSSEEDGD AHDDRSHPHT QQLLEQDGSE PLVGAAASPS SNGKRSAAGP
     SAGADVILVG ELPGDVLLDE SVGNVRRQQP HANGSGAKHN GVNGSGKSGS GGAKVAHAHV
     NGSAADTDPG QAALGVKGSA ESPLEQPAPA AKRSAAGKAS RPAALLLKRP SRSAAPPAPT
     LPDGAEADGF ASDGSGLPGH GQQQLSAIAA AAAASSLLAG PAAPMSFLET LSDDDDEARR
     AASLLNGAGM SDPASASSTS TSSNSGTSNL VGRVFVPMRL VSVSRFPLRD ALGRPLPDGL
     AVPPTGPIVL SNDARVFPGT EGLLSDYATP AGDKLAVRLE YITDRSYAYG DVALQLFNIS
     RVTRKRTDNC QMLVNGKPVN VGDPGVPLVP GDEIRFGASA AFAFRLEALP EAPSGLEAAV
     QQLQLHADSS SSNGNGSGSS SSSGLPQVSE EEVAAAAADM ASLSNMSRRD PPRAEALLRR
     LLAARPGDAA LWLIWAQMAA RVEGPGPGQA KARMLFRAAA DAARRMPVLP PPPLALQMAA
     RRATGAGRRR RRGASTTASM DGDDGALSVA DGSSSADAAI DPASGASPSA AAGPPAPSAR
     PRHNWLLVQA LGNWGKHEWR LRMYGSARHL FRAAADEAAR HSGGLAAGGG GAVMHYWGSR
     ELEAGNVRNA RIVAAEALRK CPADVALYVL AASVELEASN LELAKGYCQR AYALDRTDKQ
     LFLIWPRVEA GLGDRDKARL LFERALDAHP LNTKIINMYA RFEAEEGSYR EAAELYDRAL
     QIDPLSPGPG VHNRADWASM ETDLGNTGLA RQLLEEGLEA HPNSAALLVV YSKLQRLEGR
     YQEALAAVRR AQAVAGAFNA AVMNERAQVL RALGERELAA NLSRHVSAVK QLNRMKQQGY
     WGSEAWRAFV EATRTPEQRT LVAAARAHRL QLGWAPAVRG AKPGPPPGVV AGDGRRPAAP
     ETQQWMALEE LRRQRAEARR LTAQRTARLR AEEAAAAAGG GEAGAAAAAA ALAMGSTGSM
     GSMDGDEGYD DEIQDPVMYG ADLGSGPLPR RRLEDQDADY YEEPESMALP PLDAVRRPMP
     DADDM