NAC32_ARATH
ID NAC32_ARATH Reviewed; 253 AA.
AC Q9CAR0; Q8LAX7;
DT 02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 130.
DE RecName: Full=NAC transcription factor 32 {ECO:0000305};
DE AltName: Full=NAC domain-containing protein 32 {ECO:0000305};
DE Short=ANAC032 {ECO:0000305};
GN Name=NAC032 {ECO:0000305}; Synonyms=NAC32 {ECO:0000305};
GN OrderedLocusNames=At1g77450 {ECO:0000312|Araport:AT1G77450};
GN ORFNames=T5M16.4 {ECO:0000312|EMBL:AAG51675.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION.
RX PubMed=18849494; DOI=10.1105/tpc.108.060160;
RA Kunieda T., Mitsuda N., Ohme-Takagi M., Takeda S., Aida M., Tasaka M.,
RA Kondo M., Nishimura M., Hara-Nishimura I.;
RT "NAC family proteins NARS1/NAC2 and NARS2/NAM in the outer integument
RT regulate embryogenesis in Arabidopsis.";
RL Plant Cell 20:2631-2642(2008).
RN [7]
RP FUNCTION, AND INDUCTION BY ABSCISIC ACID.
RX PubMed=26518251; DOI=10.1111/tpj.13067;
RA Takasaki H., Maruyama K., Takahashi F., Fujita M., Yoshida T.,
RA Nakashima K., Myouga F., Toyooka K., Yamaguchi-Shinozaki K., Shinozaki K.;
RT "SNAC-As, stress-responsive NAC transcription factors, mediate ABA-
RT inducible leaf senescence.";
RL Plant J. 84:1114-1123(2015).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=27388337; DOI=10.1093/pcp/pcw120;
RA Mahmood K., El-Kereamy A., Kim S.H., Nambara E., Rothstein S.J.;
RT "ANAC032 positively regulates age-dependent and stress-induced senescence
RT in Arabidopsis thaliana.";
RL Plant Cell Physiol. 57:2029-2046(2016).
CC -!- FUNCTION: Transcriptional activator that positively regulates age-
CC dependent senescence, dark-induced leaf senescence and stress-induced
CC senescence. Regulates leaf senescence through the modulation of the
CC expression of senescence-associated genes SGR1/NYE1, SAG113 and
CC SAUR36/SAG201, which are involved in chlorophyll degradation, and
CC abscisic acid (ABA) and auxin promotion of senescence, respectively.
CC Promotes reactive oxygen species (ROS) production during age-dependent
CC and stress-induced senescence. Regulates positively auxin-mediated
CC responses in roots (PubMed:27388337). Stress-responsive NAC
CC transcription factor involved in ABA-inducible leaf senescence
CC signaling (PubMed:26518251). Required for normal seed development and
CC morphology (PubMed:18849494). {ECO:0000269|PubMed:18849494,
CC ECO:0000269|PubMed:26518251, ECO:0000269|PubMed:27388337}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00353,
CC ECO:0000269|PubMed:27388337}.
CC -!- TISSUE SPECIFICITY: Expressed in germinating seeds, roots, leaf veins,
CC open flowers and silique stalks. {ECO:0000269|PubMed:27388337}.
CC -!- INDUCTION: By abscisic acid (ABA) (PubMed:26518251). Induced by
CC salinity and osmotic stress, and during leaf senescence
CC (PubMed:27388337). {ECO:0000269|PubMed:26518251,
CC ECO:0000269|PubMed:27388337}.
CC -!- DOMAIN: The NAC domain includes a DNA binding domain and a dimerization
CC domain. {ECO:0000255|PROSITE-ProRule:PRU00353}.
CC -!- MISCELLANEOUS: Plants silencing NAC032 produce abnormally shaped seeds.
CC {ECO:0000269|PubMed:18849494}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM65083.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC010704; AAG51675.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE35979.1; -; Genomic_DNA.
DR EMBL; AK118985; BAC43561.1; -; mRNA.
DR EMBL; BT008538; AAP40365.1; -; mRNA.
DR EMBL; AY087541; AAM65083.1; ALT_INIT; mRNA.
DR PIR; G96803; G96803.
DR RefSeq; NP_177869.1; NM_106394.3.
DR AlphaFoldDB; Q9CAR0; -.
DR SMR; Q9CAR0; -.
DR IntAct; Q9CAR0; 34.
DR STRING; 3702.AT1G77450.1; -.
DR PaxDb; Q9CAR0; -.
DR PRIDE; Q9CAR0; -.
DR ProteomicsDB; 251270; -.
DR EnsemblPlants; AT1G77450.1; AT1G77450.1; AT1G77450.
DR GeneID; 844081; -.
DR Gramene; AT1G77450.1; AT1G77450.1; AT1G77450.
DR KEGG; ath:AT1G77450; -.
DR Araport; AT1G77450; -.
DR TAIR; locus:2204685; AT1G77450.
DR eggNOG; ENOG502QVRF; Eukaryota.
DR HOGENOM; CLU_035664_3_1_1; -.
DR InParanoid; Q9CAR0; -.
DR OMA; NQMWSTT; -.
DR OrthoDB; 977765at2759; -.
DR PhylomeDB; Q9CAR0; -.
DR PRO; PR:Q9CAR0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9CAR0; baseline and differential.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0001216; F:DNA-binding transcription activator activity; IDA:TAIR.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:UniProtKB.
DR GO; GO:1900057; P:positive regulation of leaf senescence; IMP:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:1902890; P:regulation of root hair elongation; IMP:TAIR.
DR Gene3D; 2.170.150.80; -; 1.
DR InterPro; IPR003441; NAC-dom.
DR InterPro; IPR036093; NAC_dom_sf.
DR Pfam; PF02365; NAM; 1.
DR SUPFAM; SSF101941; SSF101941; 1.
DR PROSITE; PS51005; NAC; 1.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Nucleus; Reference proteome; Stress response;
KW Transcription; Transcription regulation.
FT CHAIN 1..253
FT /note="NAC transcription factor 32"
FT /id="PRO_0000437979"
FT DOMAIN 10..160
FT /note="NAC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT DNA_BIND 106..166
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT CONFLICT 31
FT /note="K -> N (in Ref. 5; AAM65083)"
FT /evidence="ECO:0000305"
FT CONFLICT 142
FT /note="R -> L (in Ref. 5; AAM65083)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 253 AA; 28744 MW; BD111F2E9BABD426 CRC64;
MMKSGADLQF PPGFRFHPTD EELVLMYLCR KCASQPIPAP IITELDLYRY DPWDLPDMAL
YGEKEWYFFS PRDRKYPNGS RPNRAAGTGY WKATGADKPI GRPKPVGIKK ALVFYSGKPP
NGEKTNWIMH EYRLADVDRS VRKKNSLRLD DWVLCRIYNK KGVIEKRRSD IEDGLKPVTD
TCPPESVARL ISGSEQAVSP EFTCSNGRLS NALDFPFNYV DAIADNEIVS RLLGGNQMWS
TTLDPLVVRQ GTF
