NAC43_ARATH
ID NAC43_ARATH Reviewed; 365 AA.
AC Q84WP6; O81033; Q5M735;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 16-MAY-2006, sequence version 2.
DT 25-MAY-2022, entry version 113.
DE RecName: Full=NAC domain-containing protein 43;
DE Short=ANAC043;
DE AltName: Full=Protein EMBRYO DEFECTIVE 2301;
DE AltName: Full=Protein NAC SECONDARY WALL THICKENING PROMOTING FACTOR 1;
GN Name=NAC043; Synonyms=EMB2301, NST1; OrderedLocusNames=At2g46770;
GN ORFNames=F19D11.5;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15029955; DOI=10.1093/dnares/10.6.239;
RA Ooka H., Satoh K., Doi K., Nagata T., Otomo Y., Murakami K., Matsubara K.,
RA Osato N., Kawai J., Carninci P., Hayashizaki Y., Suzuki K., Kojima K.,
RA Takahara Y., Yamamoto K., Kikuchi S.;
RT "Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis
RT thaliana.";
RL DNA Res. 10:239-247(2003).
RN [6]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=16214898; DOI=10.1105/tpc.105.036004;
RA Mitsuda N., Seki M., Shinozaki K., Ohme-Takagi M.;
RT "The NAC transcription factors NST1 and NST2 of Arabidopsis regulate
RT secondary wall thickenings and are required for anther dehiscence.";
RL Plant Cell 17:2993-3006(2005).
RN [7]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=17237351; DOI=10.1105/tpc.106.047043;
RA Mitsuda N., Iwase A., Yamamoto H., Yoshida M., Seki M., Shinozaki K.,
RA Ohme-Takagi M.;
RT "NAC transcription factors, NST1 and NST3, are key regulators of the
RT formation of secondary walls in woody tissues of Arabidopsis.";
RL Plant Cell 19:270-280(2007).
RN [8]
RP FUNCTION.
RX PubMed=17333250; DOI=10.1007/s00425-007-0498-y;
RA Zhong R., Richardson E.A., Ye Z.-H.;
RT "Two NAC domain transcription factors, SND1 and NST1, function redundantly
RT in regulation of secondary wall synthesis in fibers of Arabidopsis.";
RL Planta 225:1603-1611(2007).
CC -!- FUNCTION: Transcription activator of genes involved in biosynthesis of
CC secondary walls. Together with NST2 and NST3, required for the
CC secondary cell wall thickening of sclerenchymatous fibers, secondary
CC xylem (tracheary elements), and of the anther endocethium, which is
CC necessary for anther dehiscence. May also regulate the secondary cell
CC wall lignification of other tissues. {ECO:0000269|PubMed:16214898,
CC ECO:0000269|PubMed:17237351, ECO:0000269|PubMed:17333250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in various aboveground tissues undergoing
CC thickening of the lignified secondary wall such as anthers, filaments
CC of stamens, the base of carpels, styles, the boundaries between
CC siliques and pedicels, the midrib of leaf veins, and inflorescence
CC stems, specifically in interfascicular fibers (sclerenchyma), cells
CC differentiating into vascular vessels, and xylary fibers (secondary
CC xylem). {ECO:0000269|PubMed:16214898, ECO:0000269|PubMed:17237351}.
CC -!- DOMAIN: The NAC domain includes a DNA-binding domain and a dimerization
CC domain.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC33506.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC005310; AAC33506.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002685; AEC10750.1; -; Genomic_DNA.
DR EMBL; BT002931; AAO22745.1; -; mRNA.
DR EMBL; BT020413; AAV97804.1; -; mRNA.
DR PIR; T02678; T02678.
DR RefSeq; NP_182200.2; NM_130243.3.
DR AlphaFoldDB; Q84WP6; -.
DR SMR; Q84WP6; -.
DR BioGRID; 4625; 43.
DR IntAct; Q84WP6; 42.
DR STRING; 3702.AT2G46770.1; -.
DR iPTMnet; Q84WP6; -.
DR PaxDb; Q84WP6; -.
DR PRIDE; Q84WP6; -.
DR ProteomicsDB; 251015; -.
DR EnsemblPlants; AT2G46770.1; AT2G46770.1; AT2G46770.
DR GeneID; 819290; -.
DR Gramene; AT2G46770.1; AT2G46770.1; AT2G46770.
DR KEGG; ath:AT2G46770; -.
DR Araport; AT2G46770; -.
DR TAIR; locus:2044360; AT2G46770.
DR eggNOG; ENOG502QSBA; Eukaryota.
DR HOGENOM; CLU_035664_1_1_1; -.
DR InParanoid; Q84WP6; -.
DR OMA; CRQLACF; -.
DR OrthoDB; 1109365at2759; -.
DR PhylomeDB; Q84WP6; -.
DR PRO; PR:Q84WP6; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q84WP6; baseline and differential.
DR Genevisible; Q84WP6; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0010047; P:fruit dehiscence; IMP:TAIR.
DR GO; GO:0009834; P:plant-type secondary cell wall biogenesis; IDA:TAIR.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:TAIR.
DR Gene3D; 2.170.150.80; -; 1.
DR InterPro; IPR003441; NAC-dom.
DR InterPro; IPR036093; NAC_dom_sf.
DR Pfam; PF02365; NAM; 1.
DR SUPFAM; SSF101941; SSF101941; 1.
DR PROSITE; PS51005; NAC; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..365
FT /note="NAC domain-containing protein 43"
FT /id="PRO_0000234356"
FT DOMAIN 16..180
FT /note="NAC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT DNA_BIND 116..186
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT CONFLICT 292
FT /note="P -> H (in Ref. 3; AAO22745)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 365 AA; 40781 MW; 333921704DA95915 CRC64;
MMSKSMSISV NGQSQVPPGF RFHPTEEELL QYYLRKKVNS IEIDLDVIRD VDLNKLEPWD
IQEMCKIGTT PQNDWYFFSH KDKKYPTGTR TNRATAAGFW KATGRDKIIY SNGRRIGMRK
TLVFYKGRAP HGQKSDWIMH EYRLDDNIIS PEDVTVHEVV SIIGEASQDE GWVVCRIFKK
KNLHKTLNSP VGGASLSGGG DTPKTTSSQI FNEDTLDQFL ELMGRSCKEE LNLDPFMKLP
NLESPNSQAI NNCHVSSPDT NHNIHVSNVV DTSFVTSWAA LDRLVASQLN GPTSYSITAV
NESHVGHDHL ALPSVRSPYP SLNRSASYHA GLTQEYTPEM ELWNTTTSSL SSSPGPFCHV
SNGSG
