NAC45_ARATH
ID NAC45_ARATH Reviewed; 479 AA.
AC A4VCM0; Q9M9N8;
DT 29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 29-MAY-2007, sequence version 1.
DT 03-AUG-2022, entry version 96.
DE RecName: Full=NAC domain-containing protein 45 {ECO:0000312|EMBL:AEE73912.1};
GN Name=NAC045 {ECO:0000312|EMBL:AEE73912.1};
GN OrderedLocusNames=At3g03200 {ECO:0000312|Araport:AT3G03200};
GN ORFNames=T17B22.11 {ECO:0000312|EMBL:AAF26106.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702 {ECO:0000312|EMBL:ABP88115.1};
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Bautista-Mercan V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C.,
RA Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (APR-2007) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, INDUCTION BY APL, AND DISRUPTION PHENOTYPE.
RX PubMed=25081480; DOI=10.1126/science.1253736;
RA Furuta K.M., Yadav S.R., Lehesranta S., Belevich I., Miyashima S.,
RA Heo J.O., Vaten A., Lindgren O., De Rybel B., Van Isterdael G.,
RA Somervuo P., Lichtenberger R., Rocha R., Thitamadee S., Taehtiharju S.,
RA Auvinen P., Beeckman T., Jokitalo E., Helariutta Y.;
RT "Plant development. Arabidopsis NAC45/86 direct sieve element morphogenesis
RT culminating in enucleation.";
RL Science 345:933-937(2014).
CC -!- FUNCTION: Transcription factor directing sieve element enucleation and
CC cytosol degradation. Not required for formation of lytic vacuoles.
CC Regulates, with NAC086, the transcription of NEN1, NEN2, NEN3, NEN4,
CC RTM1, RTM2, UBP16, PLDZETA, ABCB10 and At1g26450.
CC {ECO:0000269|PubMed:25081480}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00353}.
CC -!- TISSUE SPECIFICITY: Expressed in a few sieve element cells before
CC enucleation and in phloem-pole pericycle cells.
CC {ECO:0000269|PubMed:25081480}.
CC -!- INDUCTION: Regulated by the transcription factor APL (AC Q9SAK5).
CC {ECO:0000269|PubMed:25081480}.
CC -!- DOMAIN: The NAC domain includes a DNA binding domain and a dimerization
CC domain. {ECO:0000255|PROSITE-ProRule:PRU00353}.
CC -!- DISRUPTION PHENOTYPE: No visible phenotype, due to the redundancy with
CC NAC086 (AC Q9FFI5). Nac045 and nac086 double mutants exhibit seedling
CC lethality with defective development of the protophloem sieve element.
CC {ECO:0000269|PubMed:25081480}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF26106.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC012328; AAF26106.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE73912.1; -; Genomic_DNA.
DR EMBL; BT030461; ABP88115.1; -; mRNA.
DR RefSeq; NP_186970.2; NM_111190.3.
DR AlphaFoldDB; A4VCM0; -.
DR SMR; A4VCM0; -.
DR BioGRID; 6559; 1.
DR IntAct; A4VCM0; 1.
DR STRING; 3702.AT3G03200.1; -.
DR PaxDb; A4VCM0; -.
DR PRIDE; A4VCM0; -.
DR EnsemblPlants; AT3G03200.1; AT3G03200.1; AT3G03200.
DR GeneID; 821226; -.
DR Gramene; AT3G03200.1; AT3G03200.1; AT3G03200.
DR KEGG; ath:AT3G03200; -.
DR Araport; AT3G03200; -.
DR TAIR; locus:2097720; AT3G03200.
DR eggNOG; ENOG502QV39; Eukaryota.
DR HOGENOM; CLU_024022_1_1_1; -.
DR InParanoid; A4VCM0; -.
DR OMA; HINPINQ; -.
DR OrthoDB; 697995at2759; -.
DR PhylomeDB; A4VCM0; -.
DR PRO; PR:A4VCM0; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; A4VCM0; baseline and differential.
DR Genevisible; A4VCM0; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0090603; P:sieve element differentiation; IMP:TAIR.
DR GO; GO:0090602; P:sieve element enucleation; IMP:TAIR.
DR Gene3D; 2.170.150.80; -; 1.
DR InterPro; IPR003441; NAC-dom.
DR InterPro; IPR036093; NAC_dom_sf.
DR Pfam; PF02365; NAM; 1.
DR SUPFAM; SSF101941; SSF101941; 1.
DR PROSITE; PS51005; NAC; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..479
FT /note="NAC domain-containing protein 45"
FT /id="PRO_0000430885"
FT DOMAIN 6..157
FT /note="NAC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT DNA_BIND 105..163
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
SQ SEQUENCE 479 AA; 55588 MW; DEAA24B524B74D29 CRC64;
MAPVSLPPGF RFHPTDEELI TYYLKRKING LEIELEVIAE VDLYKCEPWD LPGKSLLPSK
DQEWYFFSPR DRKYPNGSRT NRATKGGYWK ATGKDRRVSW RDRAIGTKKT LVYYRGRAPH
GIRTGWVMHE YRLDETECEP SAYGMQDAYA LCRVFKKIVI EAKPRDQHRS YVHAMSNVSG
NCSSSFDTCS DLEISSTTHQ VQNTFQPRFG NERFNSNAIS NEDWSQYYGS SYRPFPTPYK
VNTEIECSML QHNIYLPPLR VENSAFSDSD FFTSMTHNND HGVFDDFTFA ASNSNHNNSV
GDQVIHVGNY DEQLITSNRH MNQTGYIKEQ KIRSSLDNTD EDPGFHGNNT NDNIDIDDFL
SFDIYNEDNV NQIEDNEDVN TNETLDSSGF EVVEEETRFN NQMLISTYQT TKILYHQVVP
CHTLKVHVNP ISHNVEERTL FIEEDKDSWL QRAEKITKTK LTLFSLMAQQ YYKCLAIFF
