NAC46_ARATH
ID NAC46_ARATH Reviewed; 338 AA.
AC Q9SQQ6;
DT 20-DEC-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=NAC domain-containing protein 46 {ECO:0000303|PubMed:15029955};
DE Short=ANAC046 {ECO:0000303|PubMed:15029955};
GN Name=NAC046 {ECO:0000312|EMBL:AEE74032.1};
GN OrderedLocusNames=At3g04060 {ECO:0000312|Araport:AT3G04060};
GN ORFNames=T11I18.17 {ECO:0000312|EMBL:AAF05864.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15029955; DOI=10.1093/dnares/10.6.239;
RA Ooka H., Satoh K., Doi K., Nagata T., Otomo Y., Murakami K., Matsubara K.,
RA Osato N., Kawai J., Carninci P., Hayashizaki Y., Suzuki K., Kojima K.,
RA Takahara Y., Yamamoto K., Kikuchi S.;
RT "Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis
RT thaliana.";
RL DNA Res. 10:239-247(2003).
RN [6]
RP INTERACTION WITH RCD1.
RX PubMed=25348421; DOI=10.1042/bj20141045;
RA O'Shea C., Kryger M., Stender E.G., Kragelund B.B., Willemoes M.,
RA Skriver K.;
RT "Protein intrinsic disorder in Arabidopsis NAC transcription factors:
RT transcriptional activation by ANAC013 and ANAC046 and their interactions
RT with RCD1.";
RL Biochem. J. 465:281-294(2015).
RN [7]
RP FUNCTION.
RX PubMed=27021284; DOI=10.1038/srep23609;
RA Oda-Yamamizo C., Mitsuda N., Sakamoto S., Ogawa D., Ohme-Takagi M.,
RA Ohmiya A.;
RT "The NAC transcription factor ANAC046 is a positive regulator of
RT chlorophyll degradation and senescence in Arabidopsis leaves.";
RL Sci. Rep. 6:23609-23609(2016).
CC -!- FUNCTION: Transcriptional activator that acts as positive regulator of
CC leaf senescence. Activates NYC1, SGR1, SGR2 and PAO, which are genes
CC involved in chlorophyll catabolic processes. Activates senescence-
CC associated genes, such as RNS1, SAG12 and SAG13.
CC {ECO:0000269|PubMed:27021284}.
CC -!- SUBUNIT: Interacts with RCD1. {ECO:0000269|PubMed:25348421}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00353}.
CC -!- DOMAIN: The NAC domain includes a DNA binding domain and a dimerization
CC domain. {ECO:0000255|PROSITE-ProRule:PRU00353}.
CC -!- MISCELLANEOUS: Plants overexpressing NAC046 exhibit early-senescence
CC phenotype and reduced chlorophyll content. Plants silencing NAC046
CC exhibit delayed-senescence phenotype and increased chlorophyll content.
CC {ECO:0000269|PubMed:27021284}.
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DR EMBL; AC011698; AAF05864.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74032.1; -; Genomic_DNA.
DR EMBL; AF361623; AAK32791.1; -; mRNA.
DR EMBL; AY081834; AAL87404.1; -; mRNA.
DR EMBL; AY084852; AAM61417.1; -; mRNA.
DR RefSeq; NP_187056.1; NM_111277.2.
DR AlphaFoldDB; Q9SQQ6; -.
DR SMR; Q9SQQ6; -.
DR IntAct; Q9SQQ6; 1.
DR STRING; 3702.AT3G04060.1; -.
DR PaxDb; Q9SQQ6; -.
DR PRIDE; Q9SQQ6; -.
DR DNASU; 819561; -.
DR EnsemblPlants; AT3G04060.1; AT3G04060.1; AT3G04060.
DR GeneID; 819561; -.
DR Gramene; AT3G04060.1; AT3G04060.1; AT3G04060.
DR KEGG; ath:AT3G04060; -.
DR Araport; AT3G04060; -.
DR TAIR; locus:2095958; AT3G04060.
DR eggNOG; ENOG502QSPY; Eukaryota.
DR HOGENOM; CLU_035664_6_1_1; -.
DR InParanoid; Q9SQQ6; -.
DR OMA; NFPYHSV; -.
DR OrthoDB; 949787at2759; -.
DR PhylomeDB; Q9SQQ6; -.
DR PRO; PR:Q9SQQ6; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9SQQ6; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:1903648; P:positive regulation of chlorophyll catabolic process; IMP:TAIR.
DR GO; GO:1900057; P:positive regulation of leaf senescence; IMP:TAIR.
DR DisProt; DP02225; -.
DR Gene3D; 2.170.150.80; -; 1.
DR InterPro; IPR003441; NAC-dom.
DR InterPro; IPR036093; NAC_dom_sf.
DR Pfam; PF02365; NAM; 1.
DR SUPFAM; SSF101941; SSF101941; 1.
DR PROSITE; PS51005; NAC; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..338
FT /note="NAC domain-containing protein 46"
FT /id="PRO_0000442492"
FT DOMAIN 20..171
FT /note="NAC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT DNA_BIND 118..177
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
SQ SEQUENCE 338 AA; 37781 MW; 0E26D2C0045E2FE1 CRC64;
MVEEGGVVVN QGGDQEVVDL PPGFRFHPTD EEIITHYLKE KVFNIRFTAA AIGQADLNKN
EPWDLPKIAK MGEKEFYFFC QRDRKYPTGM RTNRATVSGY WKATGKDKEI FRGKGCLVGM
KKTLVFYTGR APKGEKTNWV MHEYRLDGKY SYHNLPKTAR DEWVVCRVFH KNAPSTTITT
TKQLSRIDSL DNIDHLLDFS SLPPLIDPGF LGQPGPSFSG ARQQHDLKPV LHHPTTAPVD
NTYLPTQALN FPYHSVHNSG SDFGYGAGSG NNNKGMIKLE HSLVSVSQET GLSSDVNTTA
TPEISSYPMM MNPAMMDGSK SACDGLDDLI FWEDLYTS