NAC4_ORYSJ
ID NAC4_ORYSJ Reviewed; 343 AA.
AC Q7XUV6; B9FFJ4; Q1G0X7; Q8H0I4;
DT 03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2004, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=NAC domain-containing protein 4 {ECO:0000303|PubMed:18813954};
DE Short=ONAC004 {ECO:0000303|PubMed:18813954};
DE AltName: Full=OsNAC2 {ECO:0000303|PubMed:17888111};
DE AltName: Full=Protein TILLERING 1 {ECO:0000303|PubMed:17888111};
DE Short=OsTIL1 {ECO:0000303|PubMed:17888111};
GN Name=NAC4 {ECO:0000303|PubMed:18813954};
GN Synonyms=NAC2 {ECO:0000303|PubMed:17888111},
GN TIL1 {ECO:0000303|PubMed:17888111};
GN OrderedLocusNames=Os04g0460600 {ECO:0000312|EMBL:BAS89546.1},
GN LOC_Os04g38720 {ECO:0000305};
GN ORFNames=OsJ_15058 {ECO:0000312|EMBL:EEE61128.1},
GN OSJNBa0072F16.10 {ECO:0000312|EMBL:CAD40985.2};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Hirano H.;
RT "Molecular analysis of the NAC gene in rice.";
RL Submitted (MAY-1999) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=17888111; DOI=10.1111/j.1469-8137.2007.02177.x;
RA Mao C., Ding W., Wu Y., Yu J., He X., Shou H., Wu P.;
RT "Overexpression of a NAC-domain protein promotes shoot branching in rice.";
RL New Phytol. 176:288-298(2007).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447439; DOI=10.1038/nature01183;
RA Feng Q., Zhang Y., Hao P., Wang S., Fu G., Huang Y., Li Y., Zhu J., Liu Y.,
RA Hu X., Jia P., Zhang Y., Zhao Q., Ying K., Yu S., Tang Y., Weng Q.,
RA Zhang L., Lu Y., Mu J., Lu Y., Zhang L.S., Yu Z., Fan D., Liu X., Lu T.,
RA Li C., Wu Y., Sun T., Lei H., Li T., Hu H., Guan J., Wu M., Zhang R.,
RA Zhou B., Chen Z., Chen L., Jin Z., Wang R., Yin H., Cai Z., Ren S., Lv G.,
RA Gu W., Zhu G., Tu Y., Jia J., Zhang Y., Chen J., Kang H., Chen X., Shao C.,
RA Sun Y., Hu Q., Zhang X., Zhang W., Wang L., Ding C., Sheng H., Gu J.,
RA Chen S., Ni L., Zhu F., Chen W., Lan L., Lai Y., Cheng Z., Gu M., Jiang J.,
RA Li J., Hong G., Xue Y., Han B.;
RT "Sequence and analysis of rice chromosome 4.";
RL Nature 420:316-320(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [6]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
RN [9]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=18813954; DOI=10.1007/s00438-008-0386-6;
RA Fang Y., You J., Xie K., Xie W., Xiong L.;
RT "Systematic sequence analysis and identification of tissue-specific or
RT stress-responsive genes of NAC transcription factor family in rice.";
RL Mol. Genet. Genomics 280:547-563(2008).
RN [10]
RP FUNCTION, SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=25754802; DOI=10.1111/tpj.12819;
RA Chen X., Lu S., Wang Y., Zhang X., Lv B., Luo L., Xi D., Shen J., Ma H.,
RA Ming F.;
RT "OsNAC2 encoding a NAC transcription factor that affects plant height
RT through mediating the gibberellic acid pathway in rice.";
RL Plant J. 82:302-314(2015).
RN [11]
RP FUNCTION, AND INDUCTION BY SENESCENCE.
RX PubMed=28500268; DOI=10.1104/pp.17.00542;
RA Mao C., Lu S., Lv B., Zhang B., Shen J., He J., Luo L., Xi D., Chen X.,
RA Ming F.;
RT "A rice NAC transcription factor promotes leaf senescence via ABA
RT biosynthesis.";
RL Plant Physiol. 174:1747-1763(2017).
RN [12]
RP FUNCTION, AND INDUCTION BY SALT STRESS.
RX PubMed=29436050; DOI=10.1111/tpj.13867;
RA Mao C., Ding J., Zhang B., Xi D., Ming F.;
RT "OsNAC2 positively affects salt-induced cell death and binds to the OsAP37
RT and OsCOX11 promoters.";
RL Plant J. 94:454-468(2018).
RN [13]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=29365136; DOI=10.1093/jxb/ery017;
RA Jiang D., Chen W., Dong J., Li J., Yang F., Wu Z., Zhou H., Wang W.,
RA Zhuang C.;
RT "Overexpression of miR164b-resistant OsNAC2 improves plant architecture and
RT grain yield in rice.";
RL J. Exp. Bot. 69:1533-1543(2018).
RN [14]
RP FUNCTION.
RX PubMed=31637532; DOI=10.1186/s12284-019-0334-6;
RA Jiang D., Zhou L., Chen W., Ye N., Xia J., Zhuang C.;
RT "Overexpression of a microRNA-targeted NAC transcription factor improves
RT drought and salt tolerance in rice via ABA-mediated pathways.";
RL Rice 12:76-76(2019).
RN [15]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=31389120; DOI=10.1111/pbi.13209;
RA Mao C., He J., Liu L., Deng Q., Yao X., Liu C., Qiao Y., Li P., Ming F.;
RT "OsNAC2 integrates auxin and cytokinin pathways to modulate rice root
RT development.";
RL Plant Biotechnol. J. 18:429-442(2020).
RN [16]
RP FUNCTION.
RX PubMed=34367219; DOI=10.3389/fpls.2021.699303;
RA Yu J., Mao C., Zhong Q., Yao X., Li P., Liu C., Ming F.;
RT "OsNAC2 is involved in multiple hormonal pathways to mediate germination of
RT rice seeds and establishment of seedling.";
RL Front. Plant Sci. 12:699303-699303(2021).
CC -!- FUNCTION: Transcription factor involved in the regulation of tiller bud
CC outgrowth, but does not seems to regulate tiller bud initiation
CC (PubMed:17888111). Possesses transactivation activity in yeast
CC (PubMed:17888111). Involved in the regulation of plant architecture and
CC grain yield (PubMed:29365136). Acts as negative regulator of plant
CC height and flowering time (PubMed:25754802). Regulates directly key
CC genes of the gibberellin (GA) pathway by binding to their promoters
CC (PubMed:25754802). Positively regulates leaf senescence in an age-
CC dependent manner (PubMed:28500268). Activates directly the expression
CC of the chlorophyll degradation genes SGR and NYC3 (PubMed:28500268).
CC Regulates positively the level of abscisic acid (ABA) by directly up-
CC regulating the expression of the ABA biosynthetic genes NCED3 and ZEP,
CC and down-regulating the ABA catabolic gene CYP707A5/ABA8OX1
CC (PubMed:28500268). Promotes salt-induced cell death accompanied by the
CC loss of plasma membrane integrity, nuclear DNA fragmentation, and
CC changes of caspase-like activity (PubMed:29436050). Targets genes that
CC encoded a reactive oxygen species (ROS) scavenger COX11 and a caspase-
CC like protease AP37 (PubMed:29436050). Activates the potassium efflux
CC channels GORK and SKOR (PubMed:29436050). Acts as positive regulator of
CC drought and salt tolerance through ABA-mediated pathways
CC (PubMed:31637532). Acts as negative regulator of root growth
CC (PubMed:31389120). Functions as an upstream integrator of auxin and
CC cytokinin signals that affect CROWN ROOTLESS (CRL) and cyclin-dependent
CC protein kinase (CDK) genes to regulate cell division during root
CC development (PubMed:31389120). Binds directly to the promoters of the
CC auxin inactivation-related genes GH3.6 and GH3.8, the auxin signaling-
CC related gene ARF25, and the cytokinin oxidase gene CKX4
CC (PubMed:31389120). Activates directly the expressions of the 1-
CC aminocyclopropane-1-carboxylate oxidase genes ACO1 and ACO3, enhancing
CC ethylene synthesis, and then retarding seedling establishment
CC (PubMed:34367219). {ECO:0000269|PubMed:17888111,
CC ECO:0000269|PubMed:25754802, ECO:0000269|PubMed:28500268,
CC ECO:0000269|PubMed:29365136, ECO:0000269|PubMed:29436050,
CC ECO:0000269|PubMed:31389120, ECO:0000269|PubMed:31637532,
CC ECO:0000269|PubMed:34367219}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00353,
CC ECO:0000269|PubMed:17888111, ECO:0000269|PubMed:25754802,
CC ECO:0000269|PubMed:29365136}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, tiller buds, stems, leaves,
CC lamina joints and the young husks (PubMed:17888111). Expressed in
CC embryos, coleoptiles, radicles, leaf pulvinus, ligules, panicles, palea
CC and lemma, anthers, and the internode of the peduncles
CC (PubMed:25754802). Expressed in young leaves, root meristems,
CC florescence meristems and young spikelets (PubMed:25754802).
CC {ECO:0000269|PubMed:17888111, ECO:0000269|PubMed:25754802}.
CC -!- DEVELOPMENTAL STAGE: In 5-day-old seedling, expressed in the maturation
CC zone of roots, and later in the whole root tip. In 9- to 13-day-old
CC plants, expressed mainly in the elongation zone of roots. In 14-day-old
CC plants, expressed in the whole root tip, the apex and base of lateral
CC roots, the lateral root primordia and crown roots.
CC {ECO:0000269|PubMed:31389120}.
CC -!- INDUCTION: Up-regulated during leaf senescence (PubMed:28500268).
CC Induced by salt stress (PubMed:29436050). {ECO:0000269|PubMed:28500268,
CC ECO:0000269|PubMed:29436050}.
CC -!- DOMAIN: The NAC domain includes a DNA binding domain and a dimerization
CC domain. {ECO:0000255|PROSITE-ProRule:PRU00353}.
CC -!- MISCELLANEOUS: The gain-of-function mutant plants Ostil1 (T-DNA
CC activation tagging) exhibit increased tiller number, enlarged tiller
CC angle and semidwarf phenotype (PubMed:17888111). Overexpression of NAC4
CC shortens plant height (PubMed:25754802). Plants overexpressing NAC4
CC exhibit early leaf senescence (PubMed:28500268).
CC {ECO:0000269|PubMed:17888111, ECO:0000269|PubMed:25754802,
CC ECO:0000269|PubMed:28500268}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC53811.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB028181; BAC53811.1; ALT_INIT; mRNA.
DR EMBL; DQ520641; ABF47345.2; -; mRNA.
DR EMBL; AL606460; CAD40985.2; -; Genomic_DNA.
DR EMBL; AP008210; BAF14906.1; -; Genomic_DNA.
DR EMBL; AP014960; BAS89546.1; -; Genomic_DNA.
DR EMBL; CM000141; EEE61128.1; -; Genomic_DNA.
DR EMBL; AK061745; BAG88085.1; -; mRNA.
DR EMBL; AK104626; BAG96845.1; -; mRNA.
DR RefSeq; XP_015633922.1; XM_015778436.1.
DR RefSeq; XP_015633924.1; XM_015778438.1.
DR SMR; Q7XUV6; -.
DR STRING; 39947.Q7XUV6; -.
DR PRIDE; Q7XUV6; -.
DR EnsemblPlants; Os04t0460600-02; Os04t0460600-02; Os04g0460600.
DR GeneID; 4336052; -.
DR Gramene; Os04t0460600-02; Os04t0460600-02; Os04g0460600.
DR KEGG; osa:4336052; -.
DR eggNOG; ENOG502QR2M; Eukaryota.
DR HOGENOM; CLU_035664_6_1_1; -.
DR OrthoDB; 1072945at2759; -.
DR Proteomes; UP000000763; Chromosome 4.
DR Proteomes; UP000007752; Chromosome 4.
DR Proteomes; UP000059680; Chromosome 4.
DR ExpressionAtlas; Q7XUV6; baseline and differential.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR Gene3D; 2.170.150.80; -; 1.
DR InterPro; IPR003441; NAC-dom.
DR InterPro; IPR036093; NAC_dom_sf.
DR Pfam; PF02365; NAM; 1.
DR SUPFAM; SSF101941; SSF101941; 1.
DR PROSITE; PS51005; NAC; 1.
PE 2: Evidence at transcript level;
KW Abscisic acid signaling pathway; Auxin signaling pathway; DNA-binding;
KW Ethylene signaling pathway; Gibberellin signaling pathway; Nucleus;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..343
FT /note="NAC domain-containing protein 4"
FT /id="PRO_0000456287"
FT DOMAIN 12..168
FT /note="NAC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT DNA_BIND 109..174
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
SQ SEQUENCE 343 AA; 36839 MW; E57B0D275B25F054 CRC64;
MEQHQGQAGM DLPPGFRFHP TDEELITHYL AKKVADARFA ALAVAEADLN KCEPWDLPSL
AKMGEKEWYF FCLKDRKYPT GLRTNRATES GYWKATGKDK DIFRRKALVG MKKTLVFYTG
RAPKGEKSGW VMHEYRLHGK LHAAALGFLH GKPASSKNEW VLCRVFKKSL VEVGAAGGKK
AAVVTMEMAR GGSTSSSVAD EIAMSSVVLP PLMDMSGAGA GAVDPATTAH VTCFSNALEG
QFFNPTAVHG HGGGDSSPFM ASFTQYGQLH HGVSLVQLLE SCNGYGGLVD MAASGSQLQP
AACGGERERL SASQDTGLTS DVNPEISSSS GQKFDHEAAL WGY
