NAC52_ARATH
ID NAC52_ARATH Reviewed; 451 AA.
AC Q9SQY0; F4J3T0;
DT 25-OCT-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=NAC domain containing protein 52 {ECO:0000303|PubMed:15029955};
DE Short=ANAC052 {ECO:0000303|PubMed:15029955};
DE AltName: Full=NAC domain containing protein 51 {ECO:0000303|PubMed:15029955};
DE Short=ANAC051 {ECO:0000303|PubMed:15029955};
DE AltName: Full=Protein POLLEN DEVELOPMENT DEFECTIVE 1 {ECO:0000303|PubMed:19237690};
DE AltName: Full=Protein SUPPRESSOR OF GENE SILENCING 1 {ECO:0000303|PubMed:28207953};
GN Name=NAC052 {ECO:0000303|PubMed:15029955};
GN Synonyms=NAC051 {ECO:0000303|PubMed:15029955},
GN PDD1 {ECO:0000303|PubMed:19237690}, SGS1 {ECO:0000303|PubMed:28207953};
GN OrderedLocusNames=At3g10490 {ECO:0000312|Araport:AT3G10490};
GN ORFNames=F13M14.23 {ECO:0000312|EMBL:AAG51391.1},
GN F18K10.6 {ECO:0000312|EMBL:AAF76350.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15029955; DOI=10.1093/dnares/10.6.239;
RA Ooka H., Satoh K., Doi K., Nagata T., Otomo Y., Murakami K., Matsubara K.,
RA Osato N., Kawai J., Carninci P., Hayashizaki Y., Suzuki K., Kojima K.,
RA Takahara Y., Yamamoto K., Kikuchi S.;
RT "Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis
RT thaliana.";
RL DNA Res. 10:239-247(2003).
RN [4]
RP INDUCTION BY PSEUDOMONAS SYRINGAE.
RX PubMed=16553893; DOI=10.1111/j.1365-313x.2006.02672.x;
RA Truman W., de Zabala M.T., Grant M.;
RT "Type III effectors orchestrate a complex interplay between transcriptional
RT networks to modify basal defence responses during pathogenesis and
RT resistance.";
RL Plant J. 46:14-33(2006).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=19237690; DOI=10.1534/genetics.108.090852;
RA Boavida L.C., Shuai B., Yu H.J., Pagnussat G.C., Sundaresan V.,
RA McCormick S.;
RT "A collection of Ds insertional mutants associated with defects in male
RT gametophyte development and function in Arabidopsis thaliana.";
RL Genetics 181:1369-1385(2009).
RN [6]
RP FUNCTION, AND GENE FAMILY.
RX PubMed=24045019; DOI=10.1105/tpc.113.117168;
RA De Clercq I., Vermeirssen V., Van Aken O., Vandepoele K., Murcha M.W.,
RA Law S.R., Inze A., Ng S., Ivanova A., Rombaut D., van de Cotte B.,
RA Jaspers P., Van de Peer Y., Kangasjaervi J., Whelan J., Van Breusegem F.;
RT "The membrane-bound NAC transcription factor ANAC013 functions in
RT mitochondrial retrograde regulation of the oxidative stress response in
RT Arabidopsis.";
RL Plant Cell 25:3472-3490(2013).
RN [7]
RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH JMJ14, AND SUBCELLULAR
RP LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=26617990; DOI=10.1038/celldisc.2015.3;
RA Zhang S., Zhou B., Kang Y., Cui X., Liu A., Deleris A., Greenberg M.V.C.,
RA Cui X., Qiu Q., Lu F., Wohlschlegel J.A., Jacobsen S.E., Cao X.;
RT "C-terminal domains of a histone demethylase interact with a pair of
RT transcription factors and mediate specific chromatin association.";
RL Cell Discov. 1:0-0(2015).
RN [8]
RP FUNCTION, DISRUPTION PHENOTYPE, INTERACTION WITH JMJ14 AND NAC050, TISSUE
RP SPECIFICITY, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=cv. Columbia;
RX PubMed=25578968; DOI=10.1093/nar/gku1382;
RA Ning Y.-Q., Ma Z.-Y., Huang H.-W., Mo H., Zhao T.-T., Li L., Cai T.,
RA Chen S., Ma L., He X.-J.;
RT "Two novel NAC transcription factors regulate gene expression and flowering
RT time by associating with the histone demethylase JMJ14.";
RL Nucleic Acids Res. 43:1469-1484(2015).
RN [9]
RP FUNCTION, AND MUTAGENESIS OF ARG-32.
RX PubMed=28207953; DOI=10.1111/tpj.13508;
RA Butel N., Le Masson I., Bouteiller N., Vaucheret H., Elmayan T.;
RT "sgs1: a neomorphic nac52 allele impairing post-transcriptional gene
RT silencing through SGS3 downregulation.";
RL Plant J. 90:505-519(2017).
CC -!- FUNCTION: Transcriptional repressor that binds to the motif 5'-
CC (C/T)A(C/A)G-3' in the promoter of target genes (PubMed:25578968).
CC Binds also to the 5'-CTTGNNNNNCAAG-3' consensus sequence in chromatin
CC (PubMed:26617990). Can bind to the mitochondrial dysfunction motif
CC (MDM) present in the upstream regions of mitochondrial dysfunction
CC stimulon (MDS) genes involved in mitochondrial retrograde regulation
CC (MRR) (PubMed:24045019). Together with NAC050 and JMJ14, regulates gene
CC expression and flowering time by associating with the histone
CC demethylase JMJ14, probably by the promotion of RNA-mediated gene
CC silencing (PubMed:25578968, PubMed:26617990). Regulates siRNA-dependent
CC post-transcriptional gene silencing (PTGS) through SGS3 expression
CC modulation (PubMed:28207953). Required during pollen development
CC (PubMed:19237690). {ECO:0000269|PubMed:19237690,
CC ECO:0000269|PubMed:24045019, ECO:0000269|PubMed:25578968,
CC ECO:0000269|PubMed:26617990, ECO:0000269|PubMed:28207953}.
CC -!- SUBUNIT: Interacts with JMJ14 and NAC050. {ECO:0000269|PubMed:25578968,
CC ECO:0000269|PubMed:26617990}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00353,
CC ECO:0000269|PubMed:26617990}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=NAC051 {ECO:0000303|PubMed:15029955};
CC IsoId=Q9SQY0-1; Sequence=Displayed;
CC Name=2; Synonyms=NAC052 {ECO:0000303|PubMed:15029955};
CC IsoId=Q9SQY0-2; Sequence=VSP_059199, VSP_059200;
CC -!- TISSUE SPECIFICITY: Mostly expressed in floral organs, and, at low
CC levels, in other organs. {ECO:0000269|PubMed:25578968}.
CC -!- INDUCTION: Induced by type III effector proteins (TTEs) secreted by the
CC pathogenic bacteria P.syringae pv. tomato DC3000 during basal defense.
CC {ECO:0000269|PubMed:16553893}.
CC -!- DOMAIN: The NAC domain includes a DNA binding domain and a dimerization
CC domain. {ECO:0000255|PROSITE-ProRule:PRU00353}.
CC -!- DISRUPTION PHENOTYPE: The double mutant nac050 nac052 exhibits early
CC flowering and increased H3K4 methylation on specific genes, thus
CC leading to their derepression (PubMed:25578968). Impaired pollen
CC development (PubMed:19237690). Impaired RNA-mediated gene silencing
CC (PubMed:26617990). {ECO:0000269|PubMed:19237690,
CC ECO:0000269|PubMed:25578968, ECO:0000269|PubMed:26617990}.
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DR EMBL; AC011560; AAG51391.1; -; Genomic_DNA.
DR EMBL; AC013428; AAF76350.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74915.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE74916.1; -; Genomic_DNA.
DR RefSeq; NP_566375.1; NM_111884.2. [Q9SQY0-2]
DR RefSeq; NP_850554.1; NM_180223.4. [Q9SQY0-1]
DR AlphaFoldDB; Q9SQY0; -.
DR SMR; Q9SQY0; -.
DR STRING; 3702.AT3G10490.2; -.
DR PaxDb; Q9SQY0; -.
DR PRIDE; Q9SQY0; -.
DR ProteomicsDB; 248925; -. [Q9SQY0-1]
DR EnsemblPlants; AT3G10490.1; AT3G10490.1; AT3G10490. [Q9SQY0-2]
DR EnsemblPlants; AT3G10490.2; AT3G10490.2; AT3G10490. [Q9SQY0-1]
DR GeneID; 820213; -.
DR Gramene; AT3G10490.1; AT3G10490.1; AT3G10490. [Q9SQY0-2]
DR Gramene; AT3G10490.2; AT3G10490.2; AT3G10490. [Q9SQY0-1]
DR KEGG; ath:AT3G10490; -.
DR Araport; AT3G10490; -.
DR TAIR; locus:2075835; AT3G10490.
DR eggNOG; ENOG502QWS8; Eukaryota.
DR HOGENOM; CLU_035664_13_0_1; -.
DR InParanoid; Q9SQY0; -.
DR OMA; TCTEINR; -.
DR PhylomeDB; Q9SQY0; -.
DR PRO; PR:Q9SQY0; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9SQY0; baseline and differential.
DR GO; GO:0005730; C:nucleolus; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:TAIR.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:UniProtKB.
DR GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR GO; GO:0009908; P:flower development; IEA:UniProtKB-KW.
DR GO; GO:0034720; P:histone H3-K4 demethylation; IMP:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0048573; P:photoperiodism, flowering; IMP:UniProtKB.
DR GO; GO:0009555; P:pollen development; IMP:TAIR.
DR GO; GO:0016441; P:post-transcriptional gene silencing; IMP:TAIR.
DR GO; GO:0060966; P:regulation of gene silencing by RNA; IMP:UniProtKB.
DR GO; GO:0002237; P:response to molecule of bacterial origin; IEP:UniProtKB.
DR GO; GO:0010267; P:ta-siRNA processing; IMP:TAIR.
DR GO; GO:0006351; P:transcription, DNA-templated; IDA:TAIR.
DR GO; GO:0010228; P:vegetative to reproductive phase transition of meristem; IGI:TAIR.
DR Gene3D; 2.170.150.80; -; 1.
DR InterPro; IPR003441; NAC-dom.
DR InterPro; IPR036093; NAC_dom_sf.
DR Pfam; PF02365; NAM; 1.
DR SUPFAM; SSF101941; SSF101941; 1.
DR PROSITE; PS51005; NAC; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; DNA-binding; Flowering; Nucleus;
KW Plant defense; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..451
FT /note="NAC domain containing protein 52"
FT /id="PRO_0000442195"
FT DOMAIN 27..178
FT /note="NAC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT DNA_BIND 126..184
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 255..337
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 370..400
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 272..292
FT /evidence="ECO:0000255"
FT COILED 398..446
FT /evidence="ECO:0000255"
FT COMPBIAS 255..297
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 313..337
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 229..238
FT /note="EIQSASKSLI -> IIYASSGVHL (in isoform 2)"
FT /id="VSP_059199"
FT VAR_SEQ 239..451
FT /note="Missing (in isoform 2)"
FT /id="VSP_059200"
FT MUTAGEN 32
FT /note="R->Q: In sgs1; increased transgene promoter DNA
FT methylation, reduced histone H3 methylation levels (e.g.
FT H3K4me3 and H3K36me3), reduced polymerase II (PolII)
FT occupancy and reduced transgene mRNA accumulation. Impaired
FT siRNA-dependent post-transcriptional gene silencing (PTGS)
FT through SGS3 down-regulation. Downward curling of the leaf
FT margins and early flowering."
FT /evidence="ECO:0000269|PubMed:28207953"
SQ SEQUENCE 451 AA; 51291 MW; 445CA90FBC985728 CRC64;
MGRESVAVVT APPSATAPGT ASVATSLAPG FRFHPTDEEL VSYYLKRKVL GQPVRFDAIG
EVDIYKHEPW DLAVFSRLKT RDQEWYFYSA LDKKYGNGAR MNRATNRGYW KATGKDREIR
RDILLLGMKK TLVFHSGRAP DGLRTNWVMH EYRLVEYETE KNGNLVQDAY VLCRVFHKNN
IGPPSGNRYA PFMEEEWADD EGALIPGIDV KLRLEPPPVA NGNDQMDQEI QSASKSLINI
NEPPRETAPL DIESDQQNHH ENDLKPEEHN NNNNYDENEE TLKREQMEEE ERPPRPVCVL
NKEAPLPLLQ YKRRRQSESN NNSSRNTQDH CSSTTTTVDN TTTLISSSAA ATNTAISALL
EFSLMGISDK KEKPQQPLRP HKEPLPPQTP LASPEEKVND LQKEIHQMSV ERETFKLEMM
SAEAMISILQ SRIDALRQEN EELKKNNANG Q
