NAC54_ARATH
ID NAC54_ARATH Reviewed; 310 AA.
AC Q9FRV4; Q9LIL8;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Protein CUP-SHAPED COTYLEDON 1;
DE AltName: Full=NAC domain-containing protein 1;
DE AltName: Full=NAC domain-containing protein 54;
DE Short=ANAC054;
DE AltName: Full=NAC domain-containing protein CUC1;
GN Name=NAC054; Synonyms=CUC1, NAC1; OrderedLocusNames=At3g15170;
GN ORFNames=F4B12.8;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, MUTAGENESIS OF LEU-65; LYS-123 AND
RP 306-TRP--LEU-310, DEVELOPMENTAL STAGE, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=11245578; DOI=10.1242/dev.128.7.1127;
RA Takada S., Hibara K., Ishida T., Tasaka M.;
RT "The CUP-SHAPED COTYLEDON1 gene of Arabidopsis regulates shoot apical
RT meristem formation.";
RL Development 128:1127-1135(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Quinitio C., Chen H., Kim C.J., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION.
RX PubMed=9212461; DOI=10.1105/tpc.9.6.841;
RA Aida M., Ishida T., Fukaki H., Fujisawa H., Tasaka M.;
RT "Genes involved in organ separation in Arabidopsis: an analysis of the cup-
RT shaped cotyledon mutant.";
RL Plant Cell 9:841-857(1997).
RN [6]
RP FUNCTION.
RX PubMed=10079219; DOI=10.1242/dev.126.8.1563;
RA Aida M., Ishida T., Tasaka M.;
RT "Shoot apical meristem and cotyledon formation during Arabidopsis
RT embryogenesis: interaction among the CUP-SHAPED COTYLEDON and SHOOT
RT MERISTEMLESS genes.";
RL Development 126:1563-1570(1999).
RN [7]
RP FUNCTION.
RX PubMed=10750709; DOI=10.1093/pcp/41.1.60;
RA Ishida T., Aida M., Takada S., Tasaka M.;
RT "Involvement of CUP-SHAPED COTYLEDON genes in gynoecium and ovule
RT development in Arabidopsis thaliana.";
RL Plant Cell Physiol. 41:60-67(2000).
RN [8]
RP FUNCTION.
RX PubMed=12163400; DOI=10.1242/dev.129.17.3965;
RA Aida M., Vernoux T., Furutani M., Traas J., Tasaka M.;
RT "Roles of PIN-FORMED1 and MONOPTEROS in pattern formation of the apical
RT region of the Arabidopsis embryo.";
RL Development 129:3965-3974(2002).
RN [9]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=12492830; DOI=10.1046/j.1365-313x.2002.01479.x;
RA Cary A.J., Che P., Howell S.H.;
RT "Developmental events and shoot apical meristem gene expression patterns
RT during shoot development in Arabidopsis thaliana.";
RL Plant J. 32:867-877(2002).
RN [10]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15029955; DOI=10.1093/dnares/10.6.239;
RA Ooka H., Satoh K., Doi K., Nagata T., Otomo Y., Murakami K., Matsubara K.,
RA Osato N., Kawai J., Carninci P., Hayashizaki Y., Suzuki K., Kojima K.,
RA Takahara Y., Yamamoto K., Kikuchi S.;
RT "Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis
RT thaliana.";
RL DNA Res. 10:239-247(2003).
RN [11]
RP FUNCTION.
RX PubMed=12610213; DOI=10.1093/pcp/pcg038;
RA Daimon Y., Takabe K., Tasaka M.;
RT "The CUP-SHAPED COTYLEDON genes promote adventitious shoot formation on
RT calli.";
RL Plant Cell Physiol. 44:113-121(2003).
RN [12]
RP FUNCTION.
RX PubMed=12787253; DOI=10.1046/j.1365-313x.2003.01759.x;
RA Hiratsu K., Matsui K., Koyama T., Ohme-Takagi M.;
RT "Dominant repression of target genes by chimeric repressors that include
RT the EAR motif, a repression domain, in Arabidopsis.";
RL Plant J. 34:733-739(2003).
RN [13]
RP FUNCTION.
RX PubMed=14617069; DOI=10.1046/j.1365-313x.2003.01911.x;
RA Hibara K., Takada S., Tasaka M.;
RT "CUC1 gene activates the expression of SAM-related genes to induce
RT adventitious shoot formation.";
RL Plant J. 36:687-696(2003).
RN [14]
RP FUNCTION, AND INDUCTION BY MIR164.
RX PubMed=15202996; DOI=10.1016/j.cub.2004.06.022;
RA Mallory A.C., Dugas D.V., Bartel D.P., Bartel B.;
RT "MicroRNA regulation of NAC-domain targets is required for proper formation
RT and separation of adjacent embryonic, vegetative, and floral organs.";
RL Curr. Biol. 14:1035-1046(2004).
RN [15]
RP FUNCTION, AND INDUCTION BY MIR164.
RX PubMed=15294871; DOI=10.1242/dev.01320;
RA Laufs P., Peaucelle A., Morin H., Traas J.;
RT "MicroRNA regulation of the CUC genes is required for boundary size control
RT in Arabidopsis meristems.";
RL Development 131:4311-4322(2004).
RN [16]
RP FUNCTION, MUTAGENESIS OF TRP-306 AND PHE-308, DOMAIN, TRANSACTIVATING
RP REGION, AND SUBCELLULAR LOCATION.
RX PubMed=15500463; DOI=10.1111/j.1365-313x.2004.02238.x;
RA Taoka K., Yanagimoto Y., Daimon Y., Hibara K., Aida M., Tasaka M.;
RT "The NAC domain mediates functional specificity of CUP-SHAPED COTYLEDON
RT proteins.";
RL Plant J. 40:462-473(2004).
RN [17]
RP FUNCTION, AND INDUCTION BY MIR164.
RX PubMed=15723790; DOI=10.1016/j.cub.2005.02.017;
RA Baker C.C., Sieber P., Wellmer F., Meyerowitz E.M.;
RT "The early extra petals1 mutant uncovers a role for microRNA miR164c in
RT regulating petal number in Arabidopsis.";
RL Curr. Biol. 15:303-315(2005).
RN [18]
RP INDUCTION BY BRM.
RX PubMed=16854978; DOI=10.1242/dev.02508;
RA Kwon C.S., Hibara K., Pfluger J., Bezhani S., Metha H., Aida M., Tasaka M.,
RA Wagner D.;
RT "A role for chromatin remodeling in regulation of CUC gene expression in
RT the Arabidopsis cotyledon boundary.";
RL Development 133:3223-3230(2006).
RN [19]
RP INDUCTION BY ESR2.
RX PubMed=17056621; DOI=10.1093/pcp/pcl023;
RA Ikeda Y., Banno H., Niu Q.-W., Howell S.H., Chua N.-H.;
RT "The ENHANCER OF SHOOT REGENERATION 2 gene in Arabidopsis regulates CUP-
RT SHAPED COTYLEDON 1 at the transcriptional level and controls cotyledon
RT development.";
RL Plant Cell Physiol. 47:1443-1456(2006).
RN [20]
RP FUNCTION.
RX PubMed=16798887; DOI=10.1105/tpc.106.041988;
RA Belles-Boix E., Hamant O., Witiak S.M., Morin H., Traas J., Pautot V.;
RT "KNAT6: an Arabidopsis homeobox gene involved in meristem activity and
RT organ separation.";
RL Plant Cell 18:1900-1907(2006).
RN [21]
RP FUNCTION, AND MUTAGENESIS OF GLY-23; LEU-38; GLU-75; ARG-84; GLY-99 AND
RP PRO-307.
RX PubMed=17122068; DOI=10.1105/tpc.106.045716;
RA Hibara K., Karim M.R., Takada S., Taoka K., Furutani M., Aida M.,
RA Tasaka M.;
RT "Arabidopsis CUP-SHAPED COTYLEDON3 regulates postembryonic shoot meristem
RT and organ boundary formation.";
RL Plant Cell 18:2946-2957(2006).
RN [22]
RP FUNCTION, AND INDUCTION BY MIR164.
RX PubMed=17287247; DOI=10.1242/dev.02817;
RA Sieber P., Wellmer F., Gheyselinck J., Riechmann J.L., Meyerowitz E.M.;
RT "Redundancy and specialization among plant microRNAs: role of the MIR164
RT family in developmental robustness.";
RL Development 134:1051-1060(2007).
CC -!- FUNCTION: Transcription activator of STM and KNAT6. Involved in
CC molecular mechanisms regulating shoot apical meristem (SAM) formation
CC during embryogenesis and organ separation. Required for the fusion of
CC septa of gynoecia along the length of the ovaries. Activates the shoot
CC formation in callus in a STM-dependent manner. Seems to act as an
CC inhibitor of cell division. {ECO:0000269|PubMed:10079219,
CC ECO:0000269|PubMed:10750709, ECO:0000269|PubMed:11245578,
CC ECO:0000269|PubMed:12163400, ECO:0000269|PubMed:12492830,
CC ECO:0000269|PubMed:12610213, ECO:0000269|PubMed:12787253,
CC ECO:0000269|PubMed:14617069, ECO:0000269|PubMed:15202996,
CC ECO:0000269|PubMed:15294871, ECO:0000269|PubMed:15500463,
CC ECO:0000269|PubMed:15723790, ECO:0000269|PubMed:16798887,
CC ECO:0000269|PubMed:17122068, ECO:0000269|PubMed:17287247,
CC ECO:0000269|PubMed:9212461}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00353,
CC ECO:0000269|PubMed:15500463}.
CC -!- TISSUE SPECIFICITY: Expressed in inflorescence stems, rosette leaves,
CC aerial parts of seedlings, flowers, floral buds and roots.
CC {ECO:0000269|PubMed:11245578}.
CC -!- DEVELOPMENTAL STAGE: First observed in young embryonic SAM. Later
CC confined to the boundaries between cotyledon primordia and the SAM. In
CC mature embryos, localized around first leaves primordia. Only weakly
CC present in vegetative SAM. In inflorescence, observed at the boundaries
CC between floral organ primordia. In callus, expressed during transition
CC to shoot development, with a progressive restriction to specific areas
CC corresponding to future shoot apex. {ECO:0000269|PubMed:11245578,
CC ECO:0000269|PubMed:12492830}.
CC -!- INDUCTION: By BRM, at the chromatin level, and conferring a very
CC specific spatial expression pattern. Directly induced by ESR2 in
CC response to cytokinins. Precise spatial regulation by post-
CC transcriptional repression directed by the microRNA miR164.
CC {ECO:0000269|PubMed:15202996, ECO:0000269|PubMed:15294871,
CC ECO:0000269|PubMed:15723790, ECO:0000269|PubMed:16854978,
CC ECO:0000269|PubMed:17056621, ECO:0000269|PubMed:17287247}.
CC -!- DOMAIN: The NAC domain includes a DNA-binding domain and a dimerization
CC domain, and confers the specificity of the transactivated target genes.
CC {ECO:0000269|PubMed:15500463}.
CC -!- MISCELLANEOUS: Overexpressing transgenic plants exhibit adventitious
CC shoot apical meristems.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB02571.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; BT026080; ABG48436.1; -; mRNA.
DR EMBL; AP001299; BAB02571.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE75627.1; -; Genomic_DNA.
DR EMBL; AB049069; BAB20598.1; -; mRNA.
DR RefSeq; NP_188135.1; NM_112380.3.
DR AlphaFoldDB; Q9FRV4; -.
DR SMR; Q9FRV4; -.
DR BioGRID; 6082; 3.
DR IntAct; Q9FRV4; 1.
DR STRING; 3702.AT3G15170.1; -.
DR PaxDb; Q9FRV4; -.
DR PRIDE; Q9FRV4; -.
DR ProteomicsDB; 251280; -.
DR DNASU; 820748; -.
DR EnsemblPlants; AT3G15170.1; AT3G15170.1; AT3G15170.
DR GeneID; 820748; -.
DR Gramene; AT3G15170.1; AT3G15170.1; AT3G15170.
DR KEGG; ath:AT3G15170; -.
DR Araport; AT3G15170; -.
DR TAIR; locus:2083656; AT3G15170.
DR eggNOG; ENOG502QSC9; Eukaryota.
DR HOGENOM; CLU_035664_5_2_1; -.
DR InParanoid; Q9FRV4; -.
DR OMA; NTFATEH; -.
DR OrthoDB; 1264987at2759; -.
DR PhylomeDB; Q9FRV4; -.
DR PRO; PR:Q9FRV4; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9FRV4; baseline and differential.
DR Genevisible; Q9FRV4; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0009908; P:flower development; IMP:TAIR.
DR GO; GO:0090691; P:formation of plant organ boundary; IGI:TAIR.
DR GO; GO:0048527; P:lateral root development; TAS:TAIR.
DR GO; GO:0010014; P:meristem initiation; IMP:TAIR.
DR GO; GO:0051782; P:negative regulation of cell division; IMP:TAIR.
DR GO; GO:0010072; P:primary shoot apical meristem specification; TAS:TAIR.
DR GO; GO:0010223; P:secondary shoot formation; IMP:TAIR.
DR Gene3D; 2.170.150.80; -; 1.
DR InterPro; IPR003441; NAC-dom.
DR InterPro; IPR036093; NAC_dom_sf.
DR Pfam; PF02365; NAM; 1.
DR SUPFAM; SSF101941; SSF101941; 1.
DR PROSITE; PS51005; NAC; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..310
FT /note="Protein CUP-SHAPED COTYLEDON 1"
FT /id="PRO_0000312286"
FT DOMAIN 20..172
FT /note="NAC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT DNA_BIND 119..178
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT REGION 179..210
FT /note="Involved in transactivation activity"
FT REGION 306..310
FT /note="Involved in transactivation activity"
FT MUTAGEN 23
FT /note="G->E: In cuc1-8; cup-shaped cotyledon and abnormal
FT SAM."
FT /evidence="ECO:0000269|PubMed:17122068"
FT MUTAGEN 38
FT /note="L->F: In cuc1-9; cup-shaped cotyledon and abnormal
FT SAM."
FT /evidence="ECO:0000269|PubMed:17122068"
FT MUTAGEN 65
FT /note="L->F: In cuc1-4; cup-shaped cotyledon and abnormal
FT SAM."
FT /evidence="ECO:0000269|PubMed:11245578"
FT MUTAGEN 75
FT /note="E->K: In cuc1-10; cup-shaped cotyledon and abnormal
FT SAM."
FT /evidence="ECO:0000269|PubMed:17122068"
FT MUTAGEN 84
FT /note="R->C: In cuc1-11; cup-shaped cotyledon and abnormal
FT SAM."
FT /evidence="ECO:0000269|PubMed:17122068"
FT MUTAGEN 99
FT /note="G->S: In cuc1-12; cup-shaped cotyledon and abnormal
FT SAM."
FT /evidence="ECO:0000269|PubMed:17122068"
FT MUTAGEN 123
FT /note="K->T: In cuc1-1; cup-shaped cotyledon and abnormal
FT SAM."
FT /evidence="ECO:0000269|PubMed:11245578"
FT MUTAGEN 306..310
FT /note="Missing: In cuc1-3; cup-shaped cotyledon and
FT abnormal SAM."
FT /evidence="ECO:0000269|PubMed:11245578"
FT MUTAGEN 306
FT /note="W->A: Loss of transactivation activity; when
FT associated with A-308."
FT /evidence="ECO:0000269|PubMed:15500463"
FT MUTAGEN 307
FT /note="P->L: In cuc1-6; cup-shaped cotyledon and abnormal
FT SAM."
FT /evidence="ECO:0000269|PubMed:17122068"
FT MUTAGEN 308
FT /note="F->A: Loss of transactivation activity. when
FT associated with A-306."
FT /evidence="ECO:0000269|PubMed:15500463"
SQ SEQUENCE 310 AA; 34233 MW; E75320E1E4A73238 CRC64;
MDVDVFNGWG RPRFEDESLM PPGFRFHPTD EELITYYLLK KVLDSNFSCA AISQVDLNKS
EPWELPEKAK MGEKEWYFFT LRDRKYPTGL RTNRATEAGY WKATGKDREI KSSKTKSLLG
MKKTLVFYKG RAPKGEKSCW VMHEYRLDGK FSYHYISSSA KDEWVLCKVC LKSGVVSRET
NLISSSSSSA VTGEFSSAGS AIAPIINTFA TEHVSCFSNN SAAHTDASFH TFLPAPPPSL
PPRQPRHVGD GVAFGQFLDL GSSGQIDFDA AAAAFFPNLP SLPPTVLPPP PSFAMYGGGS
PAVSVWPFTL
