NAC60_ARATH
ID NAC60_ARATH Reviewed; 335 AA.
AC Q9LXL9;
DT 01-APR-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=NAC domain-containing protein 60 {ECO:0000303|PubMed:15029955};
DE Short=ANAC060 {ECO:0000303|PubMed:15029955};
DE AltName: Full=Protein NTM1-like 5 {ECO:0000303|PubMed:17158162};
GN Name=NAC60 {ECO:0000312|EMBL:AEE77885.1};
GN Synonyms=NTL5 {ECO:0000303|PubMed:17158162};
GN OrderedLocusNames=At3g44290 {ECO:0000312|Araport:AT3G44290};
GN ORFNames=T10D17.80 {ECO:0000312|EMBL:CAB88997.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14993207; DOI=10.1101/gr.1515604;
RA Castelli V., Aury J.-M., Jaillon O., Wincker P., Clepet C., Menard M.,
RA Cruaud C., Quetier F., Scarpelli C., Schaechter V., Temple G., Caboche M.,
RA Weissenbach J., Salanoubat M.;
RT "Whole genome sequence comparisons and 'full-length' cDNA sequences: a
RT combined approach to evaluate and improve Arabidopsis genome annotation.";
RL Genome Res. 14:406-413(2004).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15029955; DOI=10.1093/dnares/10.6.239;
RA Ooka H., Satoh K., Doi K., Nagata T., Otomo Y., Murakami K., Matsubara K.,
RA Osato N., Kawai J., Carninci P., Hayashizaki Y., Suzuki K., Kojima K.,
RA Takahara Y., Yamamoto K., Kikuchi S.;
RT "Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis
RT thaliana.";
RL DNA Res. 10:239-247(2003).
RN [5]
RP GENE FAMILY, NOMENCLATURE, TISSUE SPECIFICITY, AND INDUCTION.
RX PubMed=17158162; DOI=10.1093/nar/gkl1068;
RA Kim S.Y., Kim S.G., Kim Y.S., Seo P.J., Bae M., Yoon H.K., Park C.M.;
RT "Exploring membrane-associated NAC transcription factors in Arabidopsis:
RT implications for membrane biology in genome regulation.";
RL Nucleic Acids Res. 35:203-213(2007).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, AND INDUCTION BY GLUCOSE.
RX PubMed=24625790; DOI=10.1371/journal.pgen.1004213;
RA Li P., Zhou H., Shi X., Yu B., Zhou Y., Chen S., Wang Y., Peng Y.,
RA Meyer R.C., Smeekens S.C., Teng S.;
RT "The ABI4-induced Arabidopsis ANAC060 transcription factor attenuates ABA
RT signaling and renders seedlings sugar insensitive when present in the
RT nucleus.";
RL PLoS Genet. 10:E1004213-E1004213(2014).
CC -!- FUNCTION: Transcriptional activator activated by proteolytic cleavage
CC through regulated intramembrane proteolysis (RIP) (By similarity).
CC Transcription factor involved in modulation of abscisic acid (ABA)
CC signaling. Attenuates ABA sensitivity and glucose-induced ABA
CC accumulation. Reduces the expression of ABI4 gene.
CC {ECO:0000250|UniProtKB:Q949N0, ECO:0000269|PubMed:24625790}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250|UniProtKB:Q949N0}; Single-
CC pass membrane protein {ECO:0000255}. Nucleus {ECO:0000255|PROSITE-
CC ProRule:PRU00353, ECO:0000269|PubMed:24625790}. Note=Localized
CC primarily in plasma membrane or endoplasmic reticulum membrane as
CC dormant form and, upon specific stress or signal, is processed into a
CC transcriptionally active and nuclear form after a proteolytic cleavage
CC through regulated intramembrane proteolysis (RIP).
CC {ECO:0000250|UniProtKB:Q949N0}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, rosette leaves, cauline leaves,
CC shoot apex, stems and flowers. {ECO:0000269|PubMed:17158162}.
CC -!- INDUCTION: By glucose (PubMed:24625790). Induced by salt, drought
CC stress and methyl methanesulfonate (MMS) treatment (PubMed:17158162).
CC {ECO:0000269|PubMed:17158162, ECO:0000269|PubMed:24625790}.
CC -!- DOMAIN: The NAC domain includes a DNA binding domain and a dimerization
CC domain. {ECO:0000255|PROSITE-ProRule:PRU00353}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BX823820; Type=Miscellaneous discrepancy; Note=Sequencing errors.; Evidence={ECO:0000305};
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DR EMBL; AL353865; CAB88997.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE77885.1; -; Genomic_DNA.
DR EMBL; BX823820; -; NOT_ANNOTATED_CDS; mRNA.
DR PIR; T49145; T49145.
DR RefSeq; NP_190015.1; NM_114297.3.
DR AlphaFoldDB; Q9LXL9; -.
DR SMR; Q9LXL9; -.
DR STRING; 3702.AT3G44290.1; -.
DR PaxDb; Q9LXL9; -.
DR PRIDE; Q9LXL9; -.
DR ProteomicsDB; 248927; -.
DR EnsemblPlants; AT3G44290.1; AT3G44290.1; AT3G44290.
DR GeneID; 823554; -.
DR Gramene; AT3G44290.1; AT3G44290.1; AT3G44290.
DR KEGG; ath:AT3G44290; -.
DR Araport; AT3G44290; -.
DR TAIR; locus:2095730; AT3G44290.
DR eggNOG; ENOG502QRAU; Eukaryota.
DR HOGENOM; CLU_035664_18_0_1; -.
DR InParanoid; Q9LXL9; -.
DR OrthoDB; 936963at2759; -.
DR PhylomeDB; Q9LXL9; -.
DR PRO; PR:Q9LXL9; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LXL9; baseline and differential.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0071333; P:cellular response to glucose stimulus; IDA:UniProtKB.
DR Gene3D; 2.170.150.80; -; 1.
DR InterPro; IPR003441; NAC-dom.
DR InterPro; IPR036093; NAC_dom_sf.
DR Pfam; PF02365; NAM; 1.
DR SUPFAM; SSF101941; SSF101941; 1.
DR PROSITE; PS51005; NAC; 1.
PE 2: Evidence at transcript level;
KW Activator; DNA-binding; Membrane; Nucleus; Reference proteome;
KW Stress response; Transcription; Transcription regulation; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..335
FT /note="NAC domain-containing protein 60"
FT /id="PRO_0000432445"
FT TRANSMEM 315..335
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 14..156
FT /note="NAC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT DNA_BIND 112..162
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
SQ SEQUENCE 335 AA; 38063 MW; 54895AACA7931846 CRC64;
MAAAPPIEPA VTTTFPGFKF SPTDIELISY YLKRKMDGLE RSVEIIPEVE IYNFEPWDLP
DKSIVKSDSE WFFFCARGKK YPHGSQNRRA TKIGYWKATG KERNVKSGSE VIGTKRTLVF
HIGRAPKGGR TEWLMHEYCM IGVSLDALVI CRLRRNTEFQ GSTIQKPPQP SLPLDKHVNL
RNEAISESIY GWETMVDFYL SSESGQELLS EIAESSQSSQ NPQVPSEEDF YADILRDEIV
KLDDPAVSGN TLINVPRLQT ESNTTRVLPL PDMVDKQMQS LLQKLPLQND TGEENNISMS
NCFIGIYSIK SINRARWDVV VWLLVMIAVL VFYLV
