NAC66_ARATH
ID NAC66_ARATH Reviewed; 334 AA.
AC Q9M274; Q8LGE1;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 115.
DE RecName: Full=NAC domain-containing protein 66;
DE Short=ANAC066;
DE AltName: Full=Protein NAC SECONDARY WALL THICKENING PROMOTING FACTOR 2;
GN Name=NAC066; Synonyms=NST2; OrderedLocusNames=At3g61910;
GN ORFNames=F21F14.80;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V., Troukhan M., Alexandrov N., Lu Y.-P., Flavell R., Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15029955; DOI=10.1093/dnares/10.6.239;
RA Ooka H., Satoh K., Doi K., Nagata T., Otomo Y., Murakami K., Matsubara K.,
RA Osato N., Kawai J., Carninci P., Hayashizaki Y., Suzuki K., Kojima K.,
RA Takahara Y., Yamamoto K., Kikuchi S.;
RT "Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis
RT thaliana.";
RL DNA Res. 10:239-247(2003).
RN [5]
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=16214898; DOI=10.1105/tpc.105.036004;
RA Mitsuda N., Seki M., Shinozaki K., Ohme-Takagi M.;
RT "The NAC transcription factors NST1 and NST2 of Arabidopsis regulate
RT secondary wall thickenings and are required for anther dehiscence.";
RL Plant Cell 17:2993-3006(2005).
CC -!- FUNCTION: Transcription activator of genes involved in biosynthesis of
CC secondary walls. Together with NST1, required for the secondary cell
CC wall thickening of the anther endocethium, which is necessary for
CC anther dehiscence. May also regulate the secondary cell wall
CC lignification of other tissues such as tracheary elements.
CC {ECO:0000269|PubMed:16214898}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in anthers. Also present in
CC pollen, base of siliques and inflorescence stems.
CC {ECO:0000269|PubMed:16214898}.
CC -!- DOMAIN: The NAC domain includes a DNA-binding domain and a dimerization
CC domain.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AL138642; CAB71898.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE80278.1; -; Genomic_DNA.
DR EMBL; AY084323; AAM60909.1; -; mRNA.
DR PIR; T47983; T47983.
DR RefSeq; NP_191750.1; NM_116056.2.
DR AlphaFoldDB; Q9M274; -.
DR SMR; Q9M274; -.
DR BioGRID; 10678; 216.
DR IntAct; Q9M274; 216.
DR STRING; 3702.AT3G61910.1; -.
DR PaxDb; Q9M274; -.
DR PRIDE; Q9M274; -.
DR EnsemblPlants; AT3G61910.1; AT3G61910.1; AT3G61910.
DR GeneID; 825364; -.
DR Gramene; AT3G61910.1; AT3G61910.1; AT3G61910.
DR KEGG; ath:AT3G61910; -.
DR Araport; AT3G61910; -.
DR TAIR; locus:2079562; AT3G61910.
DR eggNOG; ENOG502QSBA; Eukaryota.
DR HOGENOM; CLU_035664_1_1_1; -.
DR InParanoid; Q9M274; -.
DR OMA; LPNLECH; -.
DR OrthoDB; 1109365at2759; -.
DR PhylomeDB; Q9M274; -.
DR PRO; PR:Q9M274; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M274; baseline and differential.
DR Genevisible; Q9M274; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0009834; P:plant-type secondary cell wall biogenesis; IMP:TAIR.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:TAIR.
DR Gene3D; 2.170.150.80; -; 1.
DR InterPro; IPR003441; NAC-dom.
DR InterPro; IPR036093; NAC_dom_sf.
DR Pfam; PF02365; NAM; 1.
DR SUPFAM; SSF101941; SSF101941; 1.
DR PROSITE; PS51005; NAC; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..334
FT /note="NAC domain-containing protein 66"
FT /id="PRO_0000234357"
FT DOMAIN 11..175
FT /note="NAC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT DNA_BIND 111..181
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT CONFLICT 73
FT /note="Y -> F (in Ref. 3; AAM60909)"
FT /evidence="ECO:0000305"
FT CONFLICT 108
FT /note="G -> D (in Ref. 3; AAM60909)"
FT /evidence="ECO:0000305"
FT CONFLICT 151
FT /note="H -> D (in Ref. 3; AAM60909)"
FT /evidence="ECO:0000305"
FT CONFLICT 240
FT /note="W -> R (in Ref. 3; AAM60909)"
FT /evidence="ECO:0000305"
FT CONFLICT 286
FT /note="N -> S (in Ref. 3; AAM60909)"
FT /evidence="ECO:0000305"
FT CONFLICT 302
FT /note="N -> H (in Ref. 3; AAM60909)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 334 AA; 37862 MW; D69C15E235D89A9D CRC64;
MNISVNGQSQ VPPGFRFHPT EEELLKYYLR KKISNIKIDL DVIPDIDLNK LEPWDIQEMC
KIGTTPQNDW YFYSHKDKKY PTGTRTNRAT TVGFWKATGR DKTIYTNGDR IGMRKTLVFY
KGRAPHGQKS DWIMHEYRLD ESVLISSCGD HDVNVETCDV IGSDEGWVVC RVFKKNNLCK
NMISSSPASS VKTPSFNEET IEQLLEVMGQ SCKGEIVLDP FLKLPNLECH NNTTITSYQW
LIDDQVNNCH VSKVMDPSFI TSWAALDRLV ASQLNGPNSY SIPAVNETSQ SPYHGLNRSG
CNTGLTPDYY IPEIDLWNEA DFARTTCHLL NGSG
