NAC83_ARATH
ID NAC83_ARATH Reviewed; 252 AA.
AC Q9FY93;
DT 24-JUN-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=NAC domain-containing protein 83 {ECO:0000303|PubMed:15029955};
DE Short=ANAC083 {ECO:0000303|PubMed:15029955};
DE AltName: Full=Protein VND-INTERACTING 2 {ECO:0000303|PubMed:20388856};
GN Name=NAC083 {ECO:0000303|PubMed:15029955};
GN Synonyms=VNI2 {ECO:0000303|PubMed:20388856};
GN OrderedLocusNames=At5g13180 {ECO:0000312|Araport:AT5G13180};
GN ORFNames=T19L5.140 {ECO:0000312|EMBL:CAC05446.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15029955; DOI=10.1093/dnares/10.6.239;
RA Ooka H., Satoh K., Doi K., Nagata T., Otomo Y., Murakami K., Matsubara K.,
RA Osato N., Kawai J., Carninci P., Hayashizaki Y., Suzuki K., Kojima K.,
RA Takahara Y., Yamamoto K., Kikuchi S.;
RT "Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis
RT thaliana.";
RL DNA Res. 10:239-247(2003).
RN [5]
RP FUNCTION, INTERACTION WITH NAC007/VND4; NAC026/VND5 AND NAC030/VND7, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=20388856; DOI=10.1105/tpc.108.064048;
RA Yamaguchi M., Ohtani M., Mitsuda N., Kubo M., Ohme-Takagi M., Fukuda H.,
RA Demura T.;
RT "VND-INTERACTING2, a NAC domain transcription factor, negatively regulates
RT xylem vessel formation in Arabidopsis.";
RL Plant Cell 22:1249-1263(2010).
RN [6]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=21673078; DOI=10.1105/tpc.111.084913;
RA Yang S.-D., Seo P.J., Yoon H.-K., Park C.-M.;
RT "The Arabidopsis NAC transcription factor VNI2 integrates abscisic acid
RT signals into leaf senescence via the COR/RD genes.";
RL Plant Cell 23:2155-2168(2011).
RN [7]
RP FUNCTION, AND INTERACTION WITH MUNGBEAN YELLOW MOSAIC VIRUS AC1
RP REPLICATION-ASSOCIATED PROTEIN.
RX PubMed=24442717; DOI=10.1007/s11262-013-1028-6;
RA Suyal G., Rana V.S., Mukherjee S.K., Wajid S., Choudhury N.R.;
RT "Arabidopsis thaliana NAC083 protein interacts with Mungbean yellow mosaic
RT India virus (MYMIV) Rep protein.";
RL Virus Genes 48:486-493(2014).
CC -!- FUNCTION: Transcriptional repressor that negatively regulates the
CC expression of genes involved in xylem vessel formation. Represses the
CC transcriptional activation activity of NAC030/VND7, which regulates
CC protoxylem vessel differentiation by promoting immature xylem vessel-
CC specific genes expression (PubMed:20388856). Transcriptional activator
CC that regulates the COLD-REGULATED (COR15A and COR15B) and RESPONSIVE TO
CC DEHYDRATION (LTI78/RD29A and LTI65/RD29B) genes by binding directly to
CC their promoters. Mediates signaling crosstalk between salt stress
CC response and leaf aging process (PubMed:21673078). May play a role in
CC DNA replication of mungbean yellow mosaic virus (PubMed:24442717).
CC {ECO:0000269|PubMed:20388856, ECO:0000269|PubMed:21673078,
CC ECO:0000269|PubMed:24442717}.
CC -!- SUBUNIT: Interacts with NAC007/VND4, NAC026/VND5 and NAC030/VND7
CC (PubMed:20388856). Interacts with the mungbean yellow mosaic virus
CC (MYMV) AC1 replication-associated protein (PubMed:24442717).
CC {ECO:0000269|PubMed:20388856, ECO:0000269|PubMed:24442717}.
CC -!- INTERACTION:
CC Q9FY93; Q8L765: BPM1; NbExp=3; IntAct=EBI-4453280, EBI-540891;
CC Q9FY93; Q8H115: NAC102; NbExp=2; IntAct=EBI-4453280, EBI-1998655;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00353,
CC ECO:0000269|PubMed:21673078}.
CC -!- TISSUE SPECIFICITY: Expressed in xylem and phloem cells in roots and
CC inflorescence stems (PubMed:20388856). Highly expressed in senescent
CC leaves. Expressed in roots, and abscission and dehiscence tissues, such
CC as axils of bracts and abscission zones in cauline leaves and siliques
CC (PubMed:21673078). {ECO:0000269|PubMed:20388856,
CC ECO:0000269|PubMed:21673078}.
CC -!- DEVELOPMENTAL STAGE: Up-regulated during xylem vessel element
CC differentiation. {ECO:0000269|PubMed:20388856}.
CC -!- INDUCTION: By abscisic acid (ABA) and salt stress.
CC {ECO:0000269|PubMed:21673078}.
CC -!- DOMAIN: The NAC domain includes a DNA binding domain and a dimerization
CC domain. {ECO:0000255|PROSITE-ProRule:PRU00353}.
CC -!- DISRUPTION PHENOTYPE: Accelerated leaf aging.
CC {ECO:0000269|PubMed:21673078}.
CC -!- MISCELLANEOUS: Over-expression of NAC083 inhibits protoxylem vessel
CC formation in roots. {ECO:0000269|PubMed:20388856}.
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DR EMBL; AL391711; CAC05446.1; -; Genomic_DNA.
DR EMBL; CP002688; AED91860.1; -; Genomic_DNA.
DR EMBL; AF385734; AAK60324.1; -; mRNA.
DR EMBL; AY143964; AAN28903.1; -; mRNA.
DR RefSeq; NP_196822.1; NM_121321.4.
DR AlphaFoldDB; Q9FY93; -.
DR SMR; Q9FY93; -.
DR IntAct; Q9FY93; 5.
DR STRING; 3702.AT5G13180.1; -.
DR PaxDb; Q9FY93; -.
DR PRIDE; Q9FY93; -.
DR ProteomicsDB; 251236; -.
DR EnsemblPlants; AT5G13180.1; AT5G13180.1; AT5G13180.
DR GeneID; 831157; -.
DR Gramene; AT5G13180.1; AT5G13180.1; AT5G13180.
DR KEGG; ath:AT5G13180; -.
DR Araport; AT5G13180; -.
DR TAIR; locus:2179877; AT5G13180.
DR eggNOG; ENOG502R7WC; Eukaryota.
DR HOGENOM; CLU_035664_9_0_1; -.
DR InParanoid; Q9FY93; -.
DR OMA; DFMTKDR; -.
DR OrthoDB; 1156653at2759; -.
DR PhylomeDB; Q9FY93; -.
DR PRO; PR:Q9FY93; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FY93; baseline and differential.
DR Genevisible; Q9FY93; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0010150; P:leaf senescence; IMP:TAIR.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:TAIR.
DR GO; GO:0009737; P:response to abscisic acid; IEP:TAIR.
DR GO; GO:0009651; P:response to salt stress; IEP:TAIR.
DR GO; GO:0010089; P:xylem development; IMP:TAIR.
DR Gene3D; 2.170.150.80; -; 1.
DR InterPro; IPR003441; NAC-dom.
DR InterPro; IPR036093; NAC_dom_sf.
DR Pfam; PF02365; NAM; 1.
DR SUPFAM; SSF101941; SSF101941; 1.
DR PROSITE; PS51005; NAC; 1.
PE 1: Evidence at protein level;
KW Activator; DNA-binding; Host-virus interaction; Nucleus;
KW Reference proteome; Repressor; Stress response; Transcription;
KW Transcription regulation.
FT CHAIN 1..252
FT /note="NAC domain-containing protein 83"
FT /id="PRO_0000433241"
FT DOMAIN 14..160
FT /note="NAC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT DNA_BIND 110..166
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT REGION 165..194
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 213..226
FT /note="PEST-like"
FT /evidence="ECO:0000305|PubMed:20388856"
FT REGION 217..252
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 178..192
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 252 AA; 28570 MW; F3A3F33FA66E80F0 CRC64;
MDNVKLVKNG VLRLPPGFRF HPTDEELVVQ YLKRKVCSSP LPASIIPEFD VCRADPWDLP
GNLEKERYFF STREAKYPNG NRSNRATGSG YWKATGIDKR VVTSRGNQIV GLKKTLVFYK
GKPPHGSRTD WIMHEYRLSS SPPSSMGPTQ NWVLCRIFLK KRAGNKNDDD DGDSRNLRHN
NNNNSSDQIE IITTDQTDDK TKPIFFDFMR KERTTDLNLL PSSPSSDHAS SGVTTEIFSS
SDEETSSCNS FR
