ID   NAC86_ARATH             Reviewed;         476 AA.
AC   Q9FFI5;
DT   29-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=NAC domain-containing protein 86 {ECO:0000312|EMBL:AED92405.1};
DE   AltName: Full=NAM-like protein {ECO:0000312|EMBL:BAB10513.1};
DE   AltName: Full=No apical meristem-like protein {ECO:0000312|EMBL:BAB10513.1};
GN   Name=NAC086 {ECO:0000312|EMBL:AED92405.1};
GN   OrderedLocusNames=At5g17260 {ECO:0000312|Araport:AT5G17260};
GN   ORFNames=MKP11.11 {ECO:0000312|EMBL:BAB10513.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702 {ECO:0000312|Proteomes:UP000006548};
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=9330910; DOI=10.1093/dnares/4.3.215;
RA   Sato S., Kotani H., Nakamura Y., Kaneko T., Asamizu E., Fukami M.,
RA   Miyajima N., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 5. I. Sequence
RT   features of the 1.6 Mb regions covered by twenty physically assigned P1
RT   clones.";
RL   DNA Res. 4:215-230(1997).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX   PubMed=25081480; DOI=10.1126/science.1253736;
RA   Furuta K.M., Yadav S.R., Lehesranta S., Belevich I., Miyashima S.,
RA   Heo J.O., Vaten A., Lindgren O., De Rybel B., Van Isterdael G.,
RA   Somervuo P., Lichtenberger R., Rocha R., Thitamadee S., Taehtiharju S.,
RA   Auvinen P., Beeckman T., Jokitalo E., Helariutta Y.;
RT   "Plant development. Arabidopsis NAC45/86 direct sieve element morphogenesis
RT   culminating in enucleation.";
RL   Science 345:933-937(2014).
CC   -!- FUNCTION: Transcription factor directing sieve element enucleation and
CC       cytosol degradation. Not required for formation of lytic vacuoles.
CC       Regulates, with NAC045, the transcription of NEN1, NEN2, NEN3, NEN4,
CC       RTM1, RTM2, UBP16, PLDZETA, ABCB10 and At1g26450.
CC       {ECO:0000269|PubMed:25081480}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00353}.
CC   -!- TISSUE SPECIFICITY: Expressed in a few sieve element cells before
CC       enucleation and in phloem-pole pericycle cells.
CC       {ECO:0000269|PubMed:25081480}.
CC   -!- DOMAIN: The NAC domain includes a DNA binding domain and a dimerization
CC       domain. {ECO:0000255|PROSITE-ProRule:PRU00353}.
CC   -!- DISRUPTION PHENOTYPE: No visible phenotype, due to the redundancy with
CC       NAC045 (AC A4VCM0). Nac045 and nac086 double mutants exhibit seedling
CC       lethality with defective development of the protophloem sieve element.
CC       {ECO:0000269|PubMed:25081480}.
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DR   EMBL; AB005238; BAB10513.1; -; Genomic_DNA.
DR   EMBL; CP002688; AED92405.1; -; Genomic_DNA.
DR   RefSeq; NP_197228.1; NM_121732.2.
DR   AlphaFoldDB; Q9FFI5; -.
DR   SMR; Q9FFI5; -.
DR   STRING; 3702.AT5G17260.1; -.
DR   PaxDb; Q9FFI5; -.
DR   PRIDE; Q9FFI5; -.
DR   EnsemblPlants; AT5G17260.1; AT5G17260.1; AT5G17260.
DR   GeneID; 831591; -.
DR   Gramene; AT5G17260.1; AT5G17260.1; AT5G17260.
DR   KEGG; ath:AT5G17260; -.
DR   Araport; AT5G17260; -.
DR   TAIR; locus:2167155; AT5G17260.
DR   eggNOG; ENOG502QV39; Eukaryota.
DR   HOGENOM; CLU_024022_1_1_1; -.
DR   InParanoid; Q9FFI5; -.
DR   OMA; HDDWSQY; -.
DR   OrthoDB; 697995at2759; -.
DR   PhylomeDB; Q9FFI5; -.
DR   PRO; PR:Q9FFI5; -.
DR   Proteomes; UP000006548; Chromosome 5.
DR   ExpressionAtlas; Q9FFI5; baseline and differential.
DR   Genevisible; Q9FFI5; AT.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR   GO; GO:0090603; P:sieve element differentiation; IGI:TAIR.
DR   GO; GO:0090602; P:sieve element enucleation; IGI:TAIR.
DR   Gene3D; 2.170.150.80; -; 1.
DR   InterPro; IPR003441; NAC-dom.
DR   InterPro; IPR036093; NAC_dom_sf.
DR   Pfam; PF02365; NAM; 1.
DR   SUPFAM; SSF101941; SSF101941; 1.
DR   PROSITE; PS51005; NAC; 1.
PE   2: Evidence at transcript level;
KW   DNA-binding; Nucleus; Reference proteome; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..476
FT                   /note="NAC domain-containing protein 86"
FT                   /id="PRO_0000430886"
FT   DOMAIN          6..157
FT                   /note="NAC"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT   DNA_BIND        105..163
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
SQ   SEQUENCE   476 AA;  54829 MW;  420F91745DEF3109 CRC64;
     MAPVSLPPGF RFHPTDEELI TYYLKRKING QEIELEIIPE VDLYKCEPWD LPGKSLIPSK
     DQEWFFFSPR DRKYPNGSRT NRATKGGYWK ATGKDRRVSW RDRAIGTKKT LVYYRGRAPH
     GIRTGWVMHE YRLDESECEP SAFGMQDAYA LCRVFKKIVI EAKPRDQHQQ QHQPYVHTSS
     NISGSSSFDV CSDLEISSNT PYNTAAHIQP RFGNANAISD HDDWSQYLSQ NMPTSFSDYG
     SPYGPYLTQS KVNTEVQCEM FQHQMSLPPL RVENSQAQTS DFSKRLHQNS GQSGFDDFTF
     AASNSNQFYN SNVDDHLIHI GNLDEQSYIE EQELILPSFQ SNDQDLELYG GSRTNTIDNI
     EIDDFFSFEN QAQDNDNSNV TPNSAGFEMI GEEIIVNHKM LISTRQTTEI LYYQVVPSQI
     LKIHINPVHG NEERTMLMEE DSDDSWFQKA ENVAKMKLKQ ISLVAKRYYK CLTIIF