NAC98_ARATH
ID NAC98_ARATH Reviewed; 375 AA.
AC O04017;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1997, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Protein CUP-SHAPED COTYLEDON 2;
DE AltName: Full=NAC domain-containing protein 98;
DE Short=ANAC098;
DE AltName: Full=NAC domain-containing protein CUC2;
GN Name=NAC098; Synonyms=CUC2; OrderedLocusNames=At5g53950;
GN ORFNames=K19P17.12;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=9212461; DOI=10.1105/tpc.9.6.841;
RA Aida M., Ishida T., Fukaki H., Fujisawa H., Tasaka M.;
RT "Genes involved in organ separation in Arabidopsis: an analysis of the cup-
RT shaped cotyledon mutant.";
RL Plant Cell 9:841-857(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9501997; DOI=10.1093/dnares/4.6.401;
RA Nakamura Y., Sato S., Kaneko T., Kotani H., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. III. Sequence
RT features of the regions of 1,191,918 bp covered by seventeen physically
RT assigned P1 clones.";
RL DNA Res. 4:401-414(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Bautista V.R., Kim C.J., Chen H., Wu S.Y., De Los Reyes C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JAN-2007) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=10079219; DOI=10.1242/dev.126.8.1563;
RA Aida M., Ishida T., Tasaka M.;
RT "Shoot apical meristem and cotyledon formation during Arabidopsis
RT embryogenesis: interaction among the CUP-SHAPED COTYLEDON and SHOOT
RT MERISTEMLESS genes.";
RL Development 126:1563-1570(1999).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=10750709; DOI=10.1093/pcp/41.1.60;
RA Ishida T., Aida M., Takada S., Tasaka M.;
RT "Involvement of CUP-SHAPED COTYLEDON genes in gynoecium and ovule
RT development in Arabidopsis thaliana.";
RL Plant Cell Physiol. 41:60-67(2000).
RN [7]
RP FUNCTION.
RX PubMed=12163400; DOI=10.1242/dev.129.17.3965;
RA Aida M., Vernoux T., Furutani M., Traas J., Tasaka M.;
RT "Roles of PIN-FORMED1 and MONOPTEROS in pattern formation of the apical
RT region of the Arabidopsis embryo.";
RL Development 129:3965-3974(2002).
RN [8]
RP FUNCTION.
RX PubMed=12492830; DOI=10.1046/j.1365-313x.2002.01479.x;
RA Cary A.J., Che P., Howell S.H.;
RT "Developmental events and shoot apical meristem gene expression patterns
RT during shoot development in Arabidopsis thaliana.";
RL Plant J. 32:867-877(2002).
RN [9]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=15029955; DOI=10.1093/dnares/10.6.239;
RA Ooka H., Satoh K., Doi K., Nagata T., Otomo Y., Murakami K., Matsubara K.,
RA Osato N., Kawai J., Carninci P., Hayashizaki Y., Suzuki K., Kojima K.,
RA Takahara Y., Yamamoto K., Kikuchi S.;
RT "Comprehensive analysis of NAC family genes in Oryza sativa and Arabidopsis
RT thaliana.";
RL DNA Res. 10:239-247(2003).
RN [10]
RP FUNCTION.
RX PubMed=12610213; DOI=10.1093/pcp/pcg038;
RA Daimon Y., Takabe K., Tasaka M.;
RT "The CUP-SHAPED COTYLEDON genes promote adventitious shoot formation on
RT calli.";
RL Plant Cell Physiol. 44:113-121(2003).
RN [11]
RP FUNCTION, AND INDUCTION BY MIR164.
RX PubMed=15202996; DOI=10.1016/j.cub.2004.06.022;
RA Mallory A.C., Dugas D.V., Bartel D.P., Bartel B.;
RT "MicroRNA regulation of NAC-domain targets is required for proper formation
RT and separation of adjacent embryonic, vegetative, and floral organs.";
RL Curr. Biol. 14:1035-1046(2004).
RN [12]
RP FUNCTION, AND INDUCTION BY MIR164.
RX PubMed=15294871; DOI=10.1242/dev.01320;
RA Laufs P., Peaucelle A., Morin H., Traas J.;
RT "MicroRNA regulation of the CUC genes is required for boundary size control
RT in Arabidopsis meristems.";
RL Development 131:4311-4322(2004).
RN [13]
RP FUNCTION, MUTAGENESIS OF TRP-373 AND TYR-375, DOMAIN, TRANSACTIVATING
RP REGION, AND SUBCELLULAR LOCATION.
RX PubMed=15500463; DOI=10.1111/j.1365-313x.2004.02238.x;
RA Taoka K., Yanagimoto Y., Daimon Y., Hibara K., Aida M., Tasaka M.;
RT "The NAC domain mediates functional specificity of CUP-SHAPED COTYLEDON
RT proteins.";
RL Plant J. 40:462-473(2004).
RN [14]
RP TISSUE SPECIFICITY.
RX PubMed=15053771; DOI=10.1111/j.1365-313x.2004.02026.x;
RA Breuil-Broyer S., Morel P., de Almeida-Engler J., Coustham V., Negrutiu I.,
RA Trehin C.;
RT "High-resolution boundary analysis during Arabidopsis thaliana flower
RT development.";
RL Plant J. 38:182-192(2004).
RN [15]
RP FUNCTION, AND INDUCTION BY MIR164.
RX PubMed=15723790; DOI=10.1016/j.cub.2005.02.017;
RA Baker C.C., Sieber P., Wellmer F., Meyerowitz E.M.;
RT "The early extra petals1 mutant uncovers a role for microRNA miR164c in
RT regulating petal number in Arabidopsis.";
RL Curr. Biol. 15:303-315(2005).
RN [16]
RP INDUCTION BY BRM AND SYD.
RX PubMed=16854978; DOI=10.1242/dev.02508;
RA Kwon C.S., Hibara K., Pfluger J., Bezhani S., Metha H., Aida M., Tasaka M.,
RA Wagner D.;
RT "A role for chromatin remodeling in regulation of CUC gene expression in
RT the Arabidopsis cotyledon boundary.";
RL Development 133:3223-3230(2006).
RN [17]
RP FUNCTION.
RX PubMed=16798887; DOI=10.1105/tpc.106.041988;
RA Belles-Boix E., Hamant O., Witiak S.M., Morin H., Traas J., Pautot V.;
RT "KNAT6: an Arabidopsis homeobox gene involved in meristem activity and
RT organ separation.";
RL Plant Cell 18:1900-1907(2006).
RN [18]
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=17098808; DOI=10.1105/tpc.106.045617;
RA Nikovics K., Blein T., Peaucelle A., Ishida T., Morin H., Aida M.,
RA Laufs P.;
RT "The balance between the MIR164A and CUC2 genes controls leaf margin
RT serration in Arabidopsis.";
RL Plant Cell 18:2929-2945(2006).
RN [19]
RP FUNCTION.
RX PubMed=17122068; DOI=10.1105/tpc.106.045716;
RA Hibara K., Karim M.R., Takada S., Taoka K., Furutani M., Aida M.,
RA Tasaka M.;
RT "Arabidopsis CUP-SHAPED COTYLEDON3 regulates postembryonic shoot meristem
RT and organ boundary formation.";
RL Plant Cell 18:2946-2957(2006).
RN [20]
RP FUNCTION, DEVELOPMENTAL STAGE, AND INDUCTION BY MIR164.
RX PubMed=17251269; DOI=10.1242/dev.02774;
RA Peaucelle A., Morin H., Traas J., Laufs P.;
RT "Plants expressing a miR164-resistant CUC2 gene reveal the importance of
RT post-meristematic maintenance of phyllotaxy in Arabidopsis.";
RL Development 134:1045-1050(2007).
RN [21]
RP FUNCTION, AND INDUCTION BY MIR164.
RX PubMed=17287247; DOI=10.1242/dev.02817;
RA Sieber P., Wellmer F., Gheyselinck J., Riechmann J.L., Meyerowitz E.M.;
RT "Redundancy and specialization among plant microRNAs: role of the MIR164
RT family in developmental robustness.";
RL Development 134:1051-1060(2007).
CC -!- FUNCTION: Transcription activator of STM and KNAT6. Involved in
CC molecular mechanisms regulating shoot apical meristem (SAM) formation
CC during embryogenesis and organ separation. Required for the fusion of
CC septa of gynoecia along the length of the ovaries. Activates the shoot
CC formation in callus in a STM-dependent manner. Controls leaf margin
CC development and required for leaf serration. Involved in axillary
CC meristem initiation and separation of the meristem from the main stem.
CC Regulates the phyllotaxy throughout the plant development. Seems to act
CC as an inhibitor of cell division. {ECO:0000269|PubMed:10079219,
CC ECO:0000269|PubMed:10750709, ECO:0000269|PubMed:12163400,
CC ECO:0000269|PubMed:12492830, ECO:0000269|PubMed:12610213,
CC ECO:0000269|PubMed:15202996, ECO:0000269|PubMed:15294871,
CC ECO:0000269|PubMed:15500463, ECO:0000269|PubMed:15723790,
CC ECO:0000269|PubMed:16798887, ECO:0000269|PubMed:17098808,
CC ECO:0000269|PubMed:17122068, ECO:0000269|PubMed:17251269,
CC ECO:0000269|PubMed:17287247, ECO:0000269|PubMed:9212461}.
CC -!- INTERACTION:
CC O04017; Q9SFV2: FHA2; NbExp=3; IntAct=EBI-1998502, EBI-15191747;
CC O04017; O82277: TCP10; NbExp=3; IntAct=EBI-1998502, EBI-3133327;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00353,
CC ECO:0000269|PubMed:15500463}.
CC -!- TISSUE SPECIFICITY: Mostly expressed in buds and flowers, and, to a
CC lower extent, in the aerial parts of seedling, inflorescence and old
CC silique. In a general manner, present at the boundaries between
CC mersitems and araising primordia. {ECO:0000269|PubMed:10750709,
CC ECO:0000269|PubMed:15053771, ECO:0000269|PubMed:9212461}.
CC -!- DEVELOPMENTAL STAGE: First expressed in early to mid-globular-stage
CC embryos. In late globular stage, detected as a stripe running medially
CC across the top of the embryo. In heart stage embryo, expression is
CC restricted to a stripe between the cotyledon primordia, but confined to
CC hypodermal cells. In the bending-cotyledon stage, localized in a region
CC surrounding the SAM, that correspond to the boundary region of
CC cotyledon margins (BCM) and the boundaries between SAM and cotyledons,
CC including protoderm cells. Observed in the margins of leaf primordia,
CC and later restricted to the leaf sinus region, with a diminution in
CC outgrowing teeth. Restricted to the proximal part of mature leaves.
CC Expressed at the boundaries between the inflorescence meristem (IM) and
CC flower primordia. Once the flower starts to grow out and the internode
CC begin to elongates, restricted to the axils of the floral pedicels
CC through several nodes. Detected within floral primordia, between sepal
CC primordia and the floral meristem. Also present at the boundaries of
CC individual sepal primordia, as well as in the region surrounding each
CC petal and stamen primordium. Later detected transiently at the
CC boundaries between locules of each theca in anthers. Expression at the
CC inner part of presumtive septal regions that raises to include
CC presumptive placenta, at the tips of septal primordia, as septum grow.
CC Localized in the fused region of the septum. Found at the boundaries of
CC ovule primordia, and later at the boundary between the nucellus and the
CC chalaza. {ECO:0000269|PubMed:10079219, ECO:0000269|PubMed:10750709,
CC ECO:0000269|PubMed:17098808, ECO:0000269|PubMed:17251269}.
CC -!- INDUCTION: By BRM and SYD, at the chromatin level, and conferring a
CC very specific spatial expression pattern. Precise spatial regulation by
CC post-transcriptional repression directed by the microRNA miR164.
CC {ECO:0000269|PubMed:15202996, ECO:0000269|PubMed:15294871,
CC ECO:0000269|PubMed:15723790, ECO:0000269|PubMed:16854978,
CC ECO:0000269|PubMed:17251269, ECO:0000269|PubMed:17287247}.
CC -!- DOMAIN: The NAC domain includes a DNA-binding domain and a dimerization
CC domain, and confers the specificity of the transactivated target genes.
CC {ECO:0000269|PubMed:15500463}.
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DR EMBL; AB002560; BAA19529.1; -; Genomic_DNA.
DR EMBL; AB007644; BAB10725.1; -; Genomic_DNA.
DR EMBL; CP002688; AED96432.1; -; Genomic_DNA.
DR EMBL; BT030026; ABN04764.1; -; mRNA.
DR RefSeq; NP_200206.1; NM_124774.3.
DR AlphaFoldDB; O04017; -.
DR SMR; O04017; -.
DR BioGRID; 20722; 31.
DR IntAct; O04017; 25.
DR STRING; 3702.AT5G53950.1; -.
DR PaxDb; O04017; -.
DR PRIDE; O04017; -.
DR ProteomicsDB; 251013; -.
DR EnsemblPlants; AT5G53950.1; AT5G53950.1; AT5G53950.
DR GeneID; 835478; -.
DR Gramene; AT5G53950.1; AT5G53950.1; AT5G53950.
DR KEGG; ath:AT5G53950; -.
DR Araport; AT5G53950; -.
DR TAIR; locus:2154684; AT5G53950.
DR eggNOG; ENOG502QSC9; Eukaryota.
DR HOGENOM; CLU_035664_5_2_1; -.
DR InParanoid; O04017; -.
DR OMA; DSGYYNQ; -.
DR OrthoDB; 1264987at2759; -.
DR PhylomeDB; O04017; -.
DR PRO; PR:O04017; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; O04017; baseline and differential.
DR Genevisible; O04017; AT.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0090691; P:formation of plant organ boundary; IGI:TAIR.
DR GO; GO:0048366; P:leaf development; IMP:TAIR.
DR GO; GO:0010072; P:primary shoot apical meristem specification; IGI:TAIR.
DR GO; GO:0090709; P:regulation of timing of plant organ formation; IMP:TAIR.
DR GO; GO:0010223; P:secondary shoot formation; IMP:TAIR.
DR Gene3D; 2.170.150.80; -; 1.
DR InterPro; IPR003441; NAC-dom.
DR InterPro; IPR036093; NAC_dom_sf.
DR Pfam; PF02365; NAM; 1.
DR SUPFAM; SSF101941; SSF101941; 1.
DR PROSITE; PS51005; NAC; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..375
FT /note="Protein CUP-SHAPED COTYLEDON 2"
FT /id="PRO_0000312287"
FT DOMAIN 17..169
FT /note="NAC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT DNA_BIND 116..175
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00353"
FT REGION 175..228
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 210..247
FT /note="Involved in transactivation activity"
FT REGION 373..375
FT /note="Involved in transactivation activity"
FT COMPBIAS 186..228
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 373
FT /note="W->A: Loss of transactivation activity. when
FT associated with A-373."
FT /evidence="ECO:0000269|PubMed:15500463"
FT MUTAGEN 375
FT /note="Y->A: Loss of transactivation activity. when
FT associated with A-371."
FT /evidence="ECO:0000269|PubMed:15500463"
SQ SEQUENCE 375 AA; 41434 MW; DA2E8AD98F83D11B CRC64;
MDIPYYHYDH GGDSQYLPPG FRFHPTDEEL ITHYLLRKVL DGCFSSRAIA EVDLNKCEPW
QLPGRAKMGE KEWYFFSLRD RKYPTGLRTN RATEAGYWKA TGKDREIFSS KTCALVGMKK
TLVFYKGRAP KGEKSNWVMH EYRLEGKFSY HFISRSSKDE WVISRVFQKT TLASTGAVSE
GGGGGGATVS VSSGTGPSKK TKVPSTISRN YQEQPSSPSS VSLPPLLDPT TTLGYTDSSC
SYDSRSTNTT VTASAITEHV SCFSTVPTTT TALGLDVNSF SRLPPPLGFD FDPFPRFVSR
NVSTQSNFRS FQENFNQFPY FGSSSASTMT SAVNLPSFQG GGGVSGMNYW LPATAEENES
KVGVLHAGLD CIWNY
