NACAD_MOUSE
ID NACAD_MOUSE Reviewed; 1504 AA.
AC Q5SWP3; Q6I6F5;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=NAC-alpha domain-containing protein 1;
GN Name=Nacad; Synonyms=Kiaa0363;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Fetal brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 62-1504.
RC TISSUE=Fetal brain;
RX PubMed=14621295; DOI=10.1093/dnares/10.4.167;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: III.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:167-180(2003).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1268, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May prevent inappropriate targeting of non-secretory
CC polypeptides to the endoplasmic reticulum (ER). May bind to nascent
CC polypeptide chains as they emerge from the ribosome and block their
CC interaction with the signal recognition particle (SRP), which normally
CC targets nascent secretory peptides to the ER. May also reduce the
CC inherent affinity of ribosomes for protein translocation sites in the
CC ER membrane (M sites) (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the NAC-alpha family. {ECO:0000305}.
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DR EMBL; AL603787; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC072589; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AB182283; BAD23961.1; -; mRNA.
DR CCDS; CCDS36108.1; -.
DR RefSeq; NP_001075121.1; NM_001081652.1.
DR AlphaFoldDB; Q5SWP3; -.
DR SMR; Q5SWP3; -.
DR BioGRID; 228718; 1.
DR IntAct; Q5SWP3; 1.
DR MINT; Q5SWP3; -.
DR STRING; 10090.ENSMUSP00000049490; -.
DR iPTMnet; Q5SWP3; -.
DR PhosphoSitePlus; Q5SWP3; -.
DR MaxQB; Q5SWP3; -.
DR PaxDb; Q5SWP3; -.
DR PeptideAtlas; Q5SWP3; -.
DR PRIDE; Q5SWP3; -.
DR ProteomicsDB; 293615; -.
DR Antibodypedia; 56503; 77 antibodies from 21 providers.
DR Ensembl; ENSMUST00000045713; ENSMUSP00000049490; ENSMUSG00000041073.
DR GeneID; 192950; -.
DR KEGG; mmu:192950; -.
DR UCSC; uc007hyy.1; mouse.
DR CTD; 23148; -.
DR MGI; MGI:3603030; Nacad.
DR VEuPathDB; HostDB:ENSMUSG00000041073; -.
DR eggNOG; KOG2239; Eukaryota.
DR GeneTree; ENSGT00940000161501; -.
DR HOGENOM; CLU_005021_0_0_1; -.
DR InParanoid; Q5SWP3; -.
DR OMA; WADPGEG; -.
DR OrthoDB; 1331328at2759; -.
DR PhylomeDB; Q5SWP3; -.
DR TreeFam; TF313348; -.
DR BioGRID-ORCS; 192950; 3 hits in 71 CRISPR screens.
DR PRO; PR:Q5SWP3; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q5SWP3; protein.
DR Bgee; ENSMUSG00000041073; Expressed in cortical plate and 183 other tissues.
DR ExpressionAtlas; Q5SWP3; baseline and differential.
DR Genevisible; Q5SWP3; MM.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005854; C:nascent polypeptide-associated complex; IEA:InterPro.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0051082; F:unfolded protein binding; IBA:GO_Central.
DR GO; GO:0006612; P:protein targeting to membrane; IBA:GO_Central.
DR CDD; cd14416; UBA_NACAD; 1.
DR Gene3D; 2.20.70.30; -; 1.
DR InterPro; IPR016641; EGD2/NACA.
DR InterPro; IPR044034; NAC-like_UBA.
DR InterPro; IPR038187; NAC_A/B_dom_sf.
DR InterPro; IPR041907; NACAD_UBA.
DR InterPro; IPR002715; Nas_poly-pep-assoc_cplx_dom.
DR PANTHER; PTHR21713; PTHR21713; 2.
DR Pfam; PF19026; HYPK_UBA; 1.
DR Pfam; PF01849; NAC; 1.
DR SMART; SM01407; NAC; 1.
DR PROSITE; PS51151; NAC_AB; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Phosphoprotein; Protein transport; Reference proteome;
KW Transport.
FT CHAIN 1..1504
FT /note="NAC-alpha domain-containing protein 1"
FT /id="PRO_0000280749"
FT DOMAIN 1354..1419
FT /note="NAC-A/B"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00507"
FT REGION 127..150
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 208..261
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 315..356
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 396..436
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 460..525
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 539..565
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 701..812
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 834..1064
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1099..1366
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1430..1467
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 221..241
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 342..356
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 487..525
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 758..800
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 834..854
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 875..890
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 952..971
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 994..1009
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1013..1056
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1121..1157
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1196..1226
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1259..1273
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1306..1341
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1350..1364
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1451..1465
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 998
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O15069"
FT MOD_RES 1268
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CONFLICT 520
FT /note="C -> R (in Ref. 3; BAD23961)"
FT /evidence="ECO:0000305"
FT CONFLICT 523
FT /note="N -> S (in Ref. 3; BAD23961)"
FT /evidence="ECO:0000305"
FT CONFLICT 548
FT /note="P -> S (in Ref. 3; BAD23961)"
FT /evidence="ECO:0000305"
FT CONFLICT 575..577
FT /note="LVV -> SVA (in Ref. 3; BAD23961)"
FT /evidence="ECO:0000305"
FT CONFLICT 706
FT /note="P -> S (in Ref. 3; BAD23961)"
FT /evidence="ECO:0000305"
FT CONFLICT 819
FT /note="P -> L (in Ref. 3; BAD23961)"
FT /evidence="ECO:0000305"
FT CONFLICT 930
FT /note="P -> S (in Ref. 3; BAD23961)"
FT /evidence="ECO:0000305"
FT CONFLICT 1090
FT /note="A -> T (in Ref. 3; BAD23961)"
FT /evidence="ECO:0000305"
FT CONFLICT 1142
FT /note="L -> LDP (in Ref. 3; BAD23961)"
FT /evidence="ECO:0000305"
FT CONFLICT 1175
FT /note="E -> V (in Ref. 3; BAD23961)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1504 AA; 156764 MW; F6A2A7B272633B80 CRC64;
MSALPLALRS ARRAGARSLG LIGGRGSADV IYAPPPPSRG AVAVLATPSA LTDRGTERQG
YRQAHVQAMP GEAAGAELPL PEAGGPGSRT DHSCDAAIAT ILKGDQLEPH GLTPGPSPLA
LTFLSSKPGA RPQPEGASWD AGPSGAASAW VDPAEGSPSL VVLPEGLPLR PVPAEGPLPT
TLEPRIVMGE ETCQVIASPR AAWPVLRDRE GGHPALHPPP ELCSQGDPPV PSPPPDLESY
FTPPSTPTKS THALLPDHGP HRDAWDLEAE LLDELLDSTP ASPSGSYITA DGDSWASSPS
CSLSLLDPAE GLDFPSDWGL SPSGSVADDL EPHPAAPPEP PSSESSLSAD SSSSWSQEGH
FFDPNFLAND PMIPAALLPF RGSLIFQVEA VEVTPLPQEE EEDEEDVAAT AAAAAPAAAT
PDGDLAGEGE DDSTSASFLQ SLSDLSIIEG MDEAFAFRDD TSAASSDSDS ASYAGADDDR
LYSGEPHAQP SAQNTEQAYR SRATFPGIES TPQTSEQEIC LTNSQESVAE IAEEILTLGL
ESEAMRTPPD QQAAPGPQVE ETPTVTPWVG NKVDLVVEQV SKALPEPCQE GISTTLGCKP
LTAEAIPDLQ EGASPSLCPV LPEKKEEGQG LPSTLEYVAV ALEGPWKAEG GVTIPQDPLM
TLPPLLQSTV PTSGPESVAV VALEPQQNEG CVTVLPDVPV VLPPSPQSVD PSSGPEAMAV
ATYEFQRAKE GTPGLQDSPV AASPALQGPD PTSEPEPEVV VTSRSQQDEG IVTVPQESPT
ASSLTLQSSH PTSDQEREVA ATLGPQQAEE GVTIPQVAPV ASPSLLQGLE STSDLESVVV
GTPESQQDEG IATATQDTPV MAPPPLRGTD STSDPELIAP DTSQALQREA GHTPGTKPSV
SEAHQELGVA SGPRPVPKEG DAEPPPHSAP PASNQAQQNG SEPGYKSDSF GAPEESDSTL
STKTSEPTSC MGEKVAANMS APKQGACLEA HDGVKTHSPQ REALRSKNKR GRGTKSPGQG
NGPKSATSQG AVETCRAHSA ARSEVSQPQL RSNEDTSGPR LPVAVSVQAR LGSCPGSPAR
ATCTLSRVYA EETSRCAPPF QHLEPMLGLG SAEQPKVTPG ILNLSPDNSA GDLLTTSQNR
FLDPDPAPST LDRASQSSPG PPDPCLCPPP QKASEEEEKP PASRGPMPRA GAQGAAAITT
SGSTKPPGAR QRVSLSPHST LNPKVAPTDT KDLACIISSP CQVPPPSGTQ NPSGPREFPA
LEQKDEDSLE EDAQRAPGSG QRWESHGESS SELDEYLAPP PDAQRTPGSG QRSESHGESS
SELGEQDLSP QKSQCPAQGP AGSNEETIAK AKQSRSEKKA RKAMSKLGLR QIQGVTRITI
QKSKNILFVI AKPDVFKSPA SDTYVVFGEA KIEDLSQQVH KAAAEKFKVP SEPSALVPEL
SPGPRVRPEC EEQEEEDEEV EEAGLEPRDI ELVMAQANVT RAKAVRALKD NHSDIVNAIM
ELTM
