AROC_ARATH
ID AROC_ARATH Reviewed; 436 AA.
AC P57720; Q570J9; Q93ZI3;
DT 11-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT 06-DEC-2005, sequence version 2.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Chorismate synthase, chloroplastic;
DE EC=4.2.3.5;
DE AltName: Full=5-enolpyruvylshikimate-3-phosphate phospholyase;
DE AltName: Full=Protein EMBRYO DEFECTIVE 1144;
DE Flags: Precursor;
GN Name=EMB1144; OrderedLocusNames=At1g48850; ORFNames=F27K7.1, T24P22.3;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Cheuk R.F., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 335-436.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the last common step of the biosynthesis of
CC aromatic amino acids, produced via the shikimic acid pathway.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate +
CC phosphate; Xref=Rhea:RHEA:21020, ChEBI:CHEBI:29748,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:57701; EC=4.2.3.5;
CC -!- COFACTOR:
CC Name=FMNH2; Xref=ChEBI:CHEBI:57618; Evidence={ECO:0000250};
CC -!- PATHWAY: Metabolic intermediate biosynthesis; chorismate biosynthesis;
CC chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step
CC 7/7.
CC -!- SUBUNIT: Homotetramer. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=P57720-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the chorismate synthase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAG29740.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=AAG50662.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAD93818.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AC084242; AAG50662.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AC084414; AAG29740.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002684; AEE32356.1; -; Genomic_DNA.
DR EMBL; AY057519; AAL09759.1; -; mRNA.
DR EMBL; BT015785; AAU90075.1; -; mRNA.
DR EMBL; AK220709; BAD93818.1; ALT_INIT; mRNA.
DR PIR; C96526; C96526.
DR RefSeq; NP_564534.1; NM_103779.5. [P57720-1]
DR AlphaFoldDB; P57720; -.
DR SMR; P57720; -.
DR BioGRID; 26532; 20.
DR STRING; 3702.AT1G48850.1; -.
DR iPTMnet; P57720; -.
DR MetOSite; P57720; -.
DR PaxDb; P57720; -.
DR PRIDE; P57720; -.
DR ProteomicsDB; 246881; -. [P57720-1]
DR EnsemblPlants; AT1G48850.1; AT1G48850.1; AT1G48850. [P57720-1]
DR GeneID; 841307; -.
DR Gramene; AT1G48850.1; AT1G48850.1; AT1G48850. [P57720-1]
DR KEGG; ath:AT1G48850; -.
DR Araport; AT1G48850; -.
DR TAIR; locus:2028549; AT1G48850.
DR eggNOG; KOG4492; Eukaryota.
DR HOGENOM; CLU_034547_0_1_1; -.
DR InParanoid; P57720; -.
DR OMA; MLSINAV; -.
DR PhylomeDB; P57720; -.
DR BioCyc; ARA:AT1G48850-MON; -.
DR UniPathway; UPA00053; UER00090.
DR PRO; PR:P57720; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; P57720; baseline and differential.
DR Genevisible; P57720; AT.
DR GO; GO:0009507; C:chloroplast; HDA:TAIR.
DR GO; GO:0009570; C:chloroplast stroma; HDA:TAIR.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005730; C:nucleolus; HDA:TAIR.
DR GO; GO:0005634; C:nucleus; HDA:TAIR.
DR GO; GO:0009536; C:plastid; HDA:TAIR.
DR GO; GO:0004107; F:chorismate synthase activity; IBA:GO_Central.
DR GO; GO:0010181; F:FMN binding; IBA:GO_Central.
DR GO; GO:0009073; P:aromatic amino acid family biosynthetic process; IBA:GO_Central.
DR GO; GO:0008652; P:cellular amino acid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0009423; P:chorismate biosynthetic process; IBA:GO_Central.
DR CDD; cd07304; Chorismate_synthase; 1.
DR Gene3D; 3.60.150.10; -; 1.
DR HAMAP; MF_00300; Chorismate_synth; 1.
DR InterPro; IPR000453; Chorismate_synth.
DR InterPro; IPR035904; Chorismate_synth_AroC_sf.
DR InterPro; IPR020541; Chorismate_synthase_CS.
DR PANTHER; PTHR21085; PTHR21085; 1.
DR Pfam; PF01264; Chorismate_synt; 1.
DR SUPFAM; SSF103263; SSF103263; 1.
DR TIGRFAMs; TIGR00033; aroC; 1.
DR PROSITE; PS00787; CHORISMATE_SYNTHASE_1; 1.
DR PROSITE; PS00788; CHORISMATE_SYNTHASE_2; 1.
DR PROSITE; PS00789; CHORISMATE_SYNTHASE_3; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Amino-acid biosynthesis;
KW Aromatic amino acid biosynthesis; Chloroplast; Lyase; Plastid;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..50
FT /note="Chloroplast"
FT /evidence="ECO:0000305"
FT CHAIN 51..436
FT /note="Chorismate synthase, chloroplastic"
FT /id="PRO_0000002294"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 436 AA; 47318 MW; 6753188EA056680F CRC64;
MASSSLTSKS ILGSTKLGSS SLPSELRRLS SPAVQISLRT QTRKNFQIQA TGSSYGTHFR
VSTFGESHGG GVGCIIDGCP PRIPLTESDL QFDLDRRRPG QSRITTPRKE TDTCRISSGV
SEGMTTGTPI HVFVPNTDQR GLDYSEMSVA YRPSHADATY DMKYGVRSVQ GGGRSSARET
IGRVAPGALA KKILKQFAGT EILAYVSQVH HVVLPEELVD HENLTLEQIE NNIVRCPNPE
YAEKMIAAID AVRTKGNSVG GVVTCIVRNA PRGLGTPVFD KLEAELAKAC MSLPATKGFE
FGSGFAGTFL TGLEHNDEFY TDENGRIRTR TNRSGGIQGG ISNGEIINMR VAFKPTSTIG
RKQNTVTRDK VETEMIARGR HDPCVVPRAV PMVEAMVALV LVDQLMAQYA QCHLFPINPE
LQEPLQIEQP QNATAL
