ID   NACB2_YEAS7             Reviewed;         149 AA.
AC   A6ZYK4;
DT   13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT   11-SEP-2007, sequence version 1.
DT   25-MAY-2022, entry version 43.
DE   RecName: Full=Nascent polypeptide-associated complex subunit beta-2;
DE            Short=NAC-beta-2;
DE   AltName: Full=BTF3 homolog BTT1;
DE   AltName: Full=Beta-2-NAC;
GN   Name=BTT1; ORFNames=SCY_1142;
OS   Saccharomyces cerevisiae (strain YJM789) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=307796;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YJM789;
RX   PubMed=17652520; DOI=10.1073/pnas.0701291104;
RA   Wei W., McCusker J.H., Hyman R.W., Jones T., Ning Y., Cao Z., Gu Z.,
RA   Bruno D., Miranda M., Nguyen M., Wilhelmy J., Komp C., Tamse R., Wang X.,
RA   Jia P., Luedi P., Oefner P.J., David L., Dietrich F.S., Li Y., Davis R.W.,
RA   Steinmetz L.M.;
RT   "Genome sequencing and comparative analysis of Saccharomyces cerevisiae
RT   strain YJM789.";
RL   Proc. Natl. Acad. Sci. U.S.A. 104:12825-12830(2007).
CC   -!- FUNCTION: Acts as component of the nascent polypeptide-associated
CC       complex (NAC), which promotes mitochondrial protein import by enhancing
CC       productive ribosome interactions with the outer mitochondrial membrane.
CC       Also blocks the inappropriate interaction of ribosomes translating non-
CC       secretory nascent polypeptides with translocation sites in the membrane
CC       of the endoplasmic reticulum. BTT1 may act as a transcription factor
CC       that exert a negative effect on the expression of several genes that
CC       are transcribed by RNA polymerase II (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Part of the nascent polypeptide-associated complex (NAC),
CC       consisting of EGD2 and either EGD1 or BTT1. NAC associates with
CC       ribosomes via EGD1 or BTT1 (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC       Note=Predominantly cytoplasmic, may also transiently localize to the
CC       nucleus. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the NAC-beta family. {ECO:0000305}.
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DR   EMBL; AAFW02000145; EDN60584.1; -; Genomic_DNA.
DR   AlphaFoldDB; A6ZYK4; -.
DR   SMR; A6ZYK4; -.
DR   EnsemblFungi; EDN60584; EDN60584; SCY_1142.
DR   HOGENOM; CLU_098726_2_2_1; -.
DR   Proteomes; UP000007060; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   Gene3D; 2.20.70.30; -; 1.
DR   InterPro; IPR039370; BTF3.
DR   InterPro; IPR038187; NAC_A/B_dom_sf.
DR   InterPro; IPR002715; Nas_poly-pep-assoc_cplx_dom.
DR   PANTHER; PTHR10351; PTHR10351; 1.
DR   Pfam; PF01849; NAC; 1.
DR   SMART; SM01407; NAC; 1.
DR   PROSITE; PS51151; NAC_AB; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Nucleus; Protein transport; Repressor; Transcription;
KW   Transcription regulation; Transport.
FT   CHAIN           1..149
FT                   /note="Nascent polypeptide-associated complex subunit beta-
FT                   2"
FT                   /id="PRO_0000310169"
FT   DOMAIN          38..103
FT                   /note="NAC-A/B"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00507"
SQ   SEQUENCE   149 AA;  16650 MW;  5EED697F1E63C909 CRC64;
     MPVDQEKLAK LHKLSAANKV GGTRRKINKK GNLYNNNDKD NTKLQAELHK LHPMTIENVA
     EANFFKKNGK VLHFNSAVVQ IAPQCNLTMI HGQPKENTLN GLYPSVASQL GSQELEYLTG
     LAHNLENEQT VLDQLSDRCS ETKQQVMNS