NACC1_MOUSE
ID NACC1_MOUSE Reviewed; 514 AA.
AC Q7TSZ8; Q80X97; Q9CZ72;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2003, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Nucleus accumbens-associated protein 1;
DE Short=NAC-1;
DE AltName: Full=BTB/POZ domain-containing protein 14B;
GN Name=Nacc1; Synonyms=Btbd14b, Nac1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP TISSUE SPECIFICITY, AND ALTERNATIVE SPLICING.
RX PubMed=14521994; DOI=10.1016/s0306-4522(03)00376-2;
RA Mackler S.A., Homan Y.X., Korutla L., Conti A.C., Blendy J.A.;
RT "The mouse Nac1 gene, encoding a cocaine-regulated bric-a-brac tramtrac
RT broad complex/pox virus and zinc finger protein, is regulated by AP1.";
RL Neuroscience 121:355-361(2003).
RN [4]
RP FUNCTION.
RX PubMed=17130457; DOI=10.1073/pnas.0604083103;
RA Nakayama K., Nakayama N., Davidson B., Sheu J.-J.C., Jinawath N.,
RA Santillan A., Salani R., Bristow R.E., Morin P.J., Kurman R.J., Wang T.-L.,
RA Shih I.-M.;
RT "A BTB/POZ protein, NAC-1, is related to tumor recurrence and is essential
RT for tumor growth and survival.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:18739-18744(2006).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17945361; DOI=10.1016/j.bbr.2007.08.036;
RA Mackler S., Pacchioni A., Degnan R., Homan Y., Conti A.C., Kalivas P.,
RA Blendy J.A.;
RT "Requirement for the POZ/BTB protein NAC1 in acute but not chronic
RT psychomotor stimulant response.";
RL Behav. Brain Res. 187:48-55(2008).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-492 AND SER-496, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, and Kidney;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Functions as a transcriptional repressor. Seems to function
CC as a transcriptional corepressor in neuronal cells through recruitment
CC of HDAC3 and HDAC4. Contributes to tumor progression, and tumor cell
CC proliferation and survival. This may be mediated at least in part
CC through repressing transcriptional activity of GADD45GIP1. Required for
CC recruiting the proteasome from the nucleus to the cytoplasm and
CC dendritic spines. Involved in the acute behavioral and neurological
CC responses to cocaine and amphetamines. {ECO:0000269|PubMed:17130457,
CC ECO:0000269|PubMed:17945361}.
CC -!- SUBUNIT: Homooligomer; mediated by the BTB domain. Interacts with HDAC3
CC and HDAC4. Interacts (via BTB domain) with CUL3, PSMD7 AND RCOR1 (By
CC similarity). {ECO:0000250}.
CC -!- INTERACTION:
CC Q7TSZ8; Q8BX22: Sall4; NbExp=4; IntAct=EBI-5691985, EBI-2312582;
CC -!- SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note=Distribution in the
CC cytoplasm is dependent on phosphorylation. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed with higher expression in
CC the brain, kidney and liver, and at lower levels in heart, lung and
CC testes. {ECO:0000269|PubMed:14521994}.
CC -!- DISRUPTION PHENOTYPE: Mice are viable with no obvious developmental or
CC physiological impairments. In addition, they do not display alterations
CC in chronic responses to cocaine and amphetamine administration, but do
CC however display significantly diminished acute behavioral and
CC neurochemical responses to these drugs. {ECO:0000269|PubMed:17945361}.
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DR EMBL; AK012935; BAB28558.1; -; mRNA.
DR EMBL; BC048157; AAH48157.1; -; mRNA.
DR EMBL; BC052706; AAH52706.1; -; mRNA.
DR EMBL; BC054800; AAH54800.1; -; mRNA.
DR CCDS; CCDS22475.1; -.
DR RefSeq; NP_080064.3; NM_025788.3.
DR RefSeq; XP_006531355.1; XM_006531292.2.
DR RefSeq; XP_006531356.1; XM_006531293.3.
DR RefSeq; XP_006531357.1; XM_006531294.3.
DR AlphaFoldDB; Q7TSZ8; -.
DR SMR; Q7TSZ8; -.
DR BioGRID; 211748; 20.
DR DIP; DIP-29930N; -.
DR IntAct; Q7TSZ8; 6.
DR MINT; Q7TSZ8; -.
DR STRING; 10090.ENSMUSP00000001975; -.
DR iPTMnet; Q7TSZ8; -.
DR PhosphoSitePlus; Q7TSZ8; -.
DR EPD; Q7TSZ8; -.
DR MaxQB; Q7TSZ8; -.
DR PaxDb; Q7TSZ8; -.
DR PeptideAtlas; Q7TSZ8; -.
DR PRIDE; Q7TSZ8; -.
DR ProteomicsDB; 287353; -.
DR Antibodypedia; 13525; 319 antibodies from 38 providers.
DR DNASU; 66830; -.
DR Ensembl; ENSMUST00000001975; ENSMUSP00000001975; ENSMUSG00000001910.
DR GeneID; 66830; -.
DR KEGG; mmu:66830; -.
DR UCSC; uc009mmu.2; mouse.
DR CTD; 112939; -.
DR MGI; MGI:1914080; Nacc1.
DR VEuPathDB; HostDB:ENSMUSG00000001910; -.
DR eggNOG; KOG1721; Eukaryota.
DR GeneTree; ENSGT00940000159327; -.
DR HOGENOM; CLU_029038_1_0_1; -.
DR InParanoid; Q7TSZ8; -.
DR OMA; SSCYFRD; -.
DR OrthoDB; 567120at2759; -.
DR PhylomeDB; Q7TSZ8; -.
DR TreeFam; TF331184; -.
DR BioGRID-ORCS; 66830; 7 hits in 73 CRISPR screens.
DR ChiTaRS; Nacc1; mouse.
DR PRO; PR:Q7TSZ8; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; Q7TSZ8; protein.
DR Bgee; ENSMUSG00000001910; Expressed in embryonic post-anal tail and 226 other tissues.
DR ExpressionAtlas; Q7TSZ8; baseline and differential.
DR Genevisible; Q7TSZ8; MM.
DR GO; GO:0030054; C:cell junction; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; ISS:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; ISS:UniProtKB.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:MGI.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR018379; BEN_domain.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR Pfam; PF10523; BEN; 1.
DR Pfam; PF00651; BTB; 1.
DR SMART; SM01025; BEN; 1.
DR SMART; SM00225; BTB; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS51457; BEN; 1.
DR PROSITE; PS50097; BTB; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Isopeptide bond; Nucleus; Phosphoprotein; Reference proteome;
KW Repressor; Transcription; Transcription regulation; Ubl conjugation.
FT CHAIN 1..514
FT /note="Nucleus accumbens-associated protein 1"
FT /id="PRO_0000274042"
FT DOMAIN 30..94
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT DOMAIN 360..457
FT /note="BEN"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00784"
FT REGION 183..205
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 242..279
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 191..205
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 242..262
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 187
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96RE7"
FT MOD_RES 245
FT /note="Phosphoserine; by PKC"
FT /evidence="ECO:0000250|UniProtKB:O35260"
FT MOD_RES 492
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 496
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT CROSSLNK 167
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO1); alternate"
FT /evidence="ECO:0000250|UniProtKB:Q96RE7"
FT CROSSLNK 167
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2); alternate"
FT /evidence="ECO:0000250|UniProtKB:Q96RE7"
FT CROSSLNK 182
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q96RE7"
FT CROSSLNK 304
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q96RE7"
FT CROSSLNK 438
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q96RE7"
FT CROSSLNK 466
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q96RE7"
FT CROSSLNK 485
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in SUMO2)"
FT /evidence="ECO:0000250|UniProtKB:Q96RE7"
FT CONFLICT 401
FT /note="S -> R (in Ref. 1; BAB28558)"
FT /evidence="ECO:0000305"
FT CONFLICT 403
FT /note="G -> V (in Ref. 2; AAH48157)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 514 AA; 56537 MW; C83C23891B097626 CRC64;
MAQTLQMEIP NFGNSILECL NEQRLQGLYC DVSVVVKGHA FKAHRAVLAA SSSYFRDLFN
SSRSAVVELP AAVQPQSFQQ ILTFCYTGRL SMNMGDQFLL IYTAGFLQIQ EIMEKGTEFF
LKVSSPSCDS QGLHPEEAPS SEPQSPVAQT LGWPACSTPL PLVSRVKTEQ ELDSVQCTPM
AKRLWDSSQK EAGGSGGNNG SRKMAKFSTP DLALNRMPQP LSMATATAAV AVVAVGGCVS
GPSMSERTSP GTSSAYTSDS PSSYHNEEDE EEDAGEEGTD EQYRQICNMY TMYSMLNVGQ
TAEKVEALPE QVVLESRSRI RVRQDLASLP AELINQIGNR CHPKLYDEGD PSEKLELVTG
TNVYITRAQL MNCHVSAGTR HKVLLRRLLA SFFDRNTLAN SCGTGIRSST NDPRRKPLDS
RVLHAVKYYC QNFAPNFKES EMNAIAADMC TNARRVVRKS WLPKTKPLHL VEGDNYSSFI
SDTCKIEPDM MSMEHSFETA SHDGEAGPSA EVLQ
