ID   NACHO_DANRE             Reviewed;         160 AA.
AC   Q58EL2;
DT   20-JAN-2009, integrated into UniProtKB/Swiss-Prot.
DT   26-APR-2005, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Novel acetylcholine receptor chaperone {ECO:0000250|UniProtKB:Q53FP2};
DE   AltName: Full=Transmembrane protein 35A;
GN   Name=tmem35a; Synonyms=nacho {ECO:0000250|UniProtKB:Q53FP2}, tmem35;
GN   ORFNames=zgc:110832;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Molecular chaperone which mediates the proper assembly and
CC       functional expression of the nicotinic acetylcholine receptors (nAChRs)
CC       throughout the brain (By similarity). Essential for the proper folding,
CC       assembly, function and surface trafficking of alpha-7 (CHRNA7), alpha-
CC       4-beta-2, alpha-3-beta-2 and alpha-3-beta-4 receptors (By similarity).
CC       {ECO:0000250|UniProtKB:Q53FP2}.
CC   -!- SUBCELLULAR LOCATION: Peroxisome membrane
CC       {ECO:0000250|UniProtKB:Q9D328}; Multi-pass membrane protein
CC       {ECO:0000255}. Cytoplasmic vesicle {ECO:0000250|UniProtKB:Q6JAM9}.
CC       Endoplasmic reticulum membrane {ECO:0000250|UniProtKB:Q9D328}; Multi-
CC       pass membrane protein {ECO:0000255}. Note=Shedding may lead to a
CC       soluble peptide. {ECO:0000250|UniProtKB:Q6JAM9}.
CC   -!- SIMILARITY: Belongs to the DoxX family. {ECO:0000305}.
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DR   EMBL; BC091856; AAH91856.1; -; mRNA.
DR   AlphaFoldDB; Q58EL2; -.
DR   STRING; 7955.ENSDARP00000068937; -.
DR   PaxDb; Q58EL2; -.
DR   ZFIN; ZDB-GENE-030815-1; tmem35.
DR   eggNOG; ENOG502RXPR; Eukaryota.
DR   InParanoid; Q58EL2; -.
DR   PhylomeDB; Q58EL2; -.
DR   PRO; PR:Q58EL2; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Unplaced.
DR   GO; GO:0031410; C:cytoplasmic vesicle; IEA:UniProtKB-KW.
DR   GO; GO:0005783; C:endoplasmic reticulum; ISS:UniProtKB.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005778; C:peroxisomal membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0030548; F:acetylcholine receptor regulator activity; IBA:GO_Central.
DR   GO; GO:0051131; P:chaperone-mediated protein complex assembly; ISS:UniProtKB.
DR   GO; GO:2000010; P:positive regulation of protein localization to cell surface; ISS:UniProtKB.
DR   InterPro; IPR032808; DoxX.
DR   InterPro; IPR029693; TMEM35A.
DR   InterPro; IPR040399; TMEM35A/B.
DR   PANTHER; PTHR13163; PTHR13163; 1.
DR   PANTHER; PTHR13163:SF0; PTHR13163:SF0; 1.
DR   Pfam; PF13564; DoxX_2; 1.
PE   2: Evidence at transcript level;
KW   Chaperone; Cytoplasmic vesicle; Endoplasmic reticulum; Membrane;
KW   Peroxisome; Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..160
FT                   /note="Novel acetylcholine receptor chaperone"
FT                   /id="PRO_0000359757"
FT   TOPO_DOM        1..5
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        6..26
FT                   /note="Helical; Name=1"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        27..61
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        62..82
FT                   /note="Helical; Name=2"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        83..88
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        89..109
FT                   /note="Helical; Name=3"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        110..113
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000305"
FT   TRANSMEM        114..131
FT                   /note="Helical; Name=4"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        132..160
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000305"
FT   REGION          137..160
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   160 AA;  17887 MW;  F2716D67DFE87B06 CRC64;
     MASPRTVTIV ALSFALGLFF VFMGTIKLTP RLSKDAYSEM KRAYKSYAKA LPALKKMGVS
     SVLLRKIIGT LEVGCGIVLT LVPGRPKDVA NFILLLVMLA VLFFHQLVGD PLKRYAHALV
     FGILLTCRLL IARQAEDRPE REERREEQIN AQEKNKVKVS