NACH_DROME
ID NACH_DROME Reviewed; 535 AA.
AC O61365; Q7KRC7; Q86LH4; Q9V7S4;
DT 10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 10-OCT-2003, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Sodium channel protein Nach;
DE AltName: Full=Pickpocket protein 4;
GN Name=Nach; Synonyms=PPK4; ORFNames=CG8178;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAO47364.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE (ISOFORM A).
RC STRAIN=Canton-S {ECO:0000305};
RA Da Lage J.-L.;
RL Submitted (SEP-1997) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=12571352; DOI=10.1073/pnas.252785099;
RA Liu L., Johnson W.A., Welsh M.J.;
RT "Drosophila DEG/ENaC pickpocket genes are expressed in the tracheal system,
RT where they may be involved in liquid clearance.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:2128-2133(2003).
RN [3] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION, AND ALTERNATIVE SPLICING.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
CC -!- FUNCTION: Part of a complex that plays a role in tracheal liquid
CC clearance. Probable role in sodium transport.
CC {ECO:0000269|PubMed:12571352, ECO:0000303|PubMed:12571352}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=B {ECO:0000269|PubMed:12571352}; Synonyms=PPK4L;
CC IsoId=O61365-1; Sequence=Displayed;
CC Name=A {ECO:0000305};
CC IsoId=O61365-2; Sequence=VSP_008551;
CC Name=C;
CC IsoId=O61365-3; Sequence=VSP_011421;
CC -!- TISSUE SPECIFICITY: Embryonic and larval tracheal system; dorsal trunk
CC (but not at fusion with transverse connective), several branches and
CC terminal cells. Also expressed in adult tracheal system; dorsal trunk,
CC but not at the spiracles. {ECO:0000269|PubMed:12571352}.
CC -!- SIMILARITY: Belongs to the amiloride-sensitive sodium channel (TC
CC 1.A.6) family. {ECO:0000305}.
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DR EMBL; AF022713; AAD09149.1; -; Genomic_DNA.
DR EMBL; AY226538; AAO47364.1; -; mRNA.
DR EMBL; AE013599; AAF57970.1; -; Genomic_DNA.
DR EMBL; AE013599; AAS64839.1; -; Genomic_DNA.
DR EMBL; AE013599; AAS64840.1; -; Genomic_DNA.
DR RefSeq; NP_001334723.1; NM_001347816.1. [O61365-1]
DR RefSeq; NP_611145.1; NM_137301.1. [O61365-2]
DR AlphaFoldDB; O61365; -.
DR BioGRID; 62576; 1.
DR STRING; 7227.FBpp0088959; -.
DR TCDB; 1.A.6.4.3; the epithelial na(+) channel (enac) family.
DR GlyGen; O61365; 3 sites.
DR PaxDb; O61365; -.
DR EnsemblMetazoa; FBtr0087116; FBpp0086262; FBgn0024319. [O61365-2]
DR EnsemblMetazoa; FBtr0445749; FBpp0401612; FBgn0024319. [O61365-1]
DR EnsemblMetazoa; FBtr0445750; FBpp0401613; FBgn0024319. [O61365-3]
DR GeneID; 36864; -.
DR KEGG; dme:Dmel_CG8178; -.
DR CTD; 36864; -.
DR FlyBase; FBgn0024319; Nach.
DR VEuPathDB; VectorBase:FBgn0024319; -.
DR eggNOG; KOG4294; Eukaryota.
DR GeneTree; ENSGT00940000172566; -.
DR HOGENOM; CLU_024950_1_1_1; -.
DR InParanoid; O61365; -.
DR OMA; YVYPNCE; -.
DR PhylomeDB; O61365; -.
DR Reactome; R-DME-2672351; Stimuli-sensing channels.
DR BioGRID-ORCS; 36864; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 36864; -.
DR PRO; PR:O61365; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0024319; Expressed in open tracheal system trachea and 9 other tissues.
DR ExpressionAtlas; O61365; differential.
DR Genevisible; O61365; DM.
DR GO; GO:0016021; C:integral component of membrane; ISS:FlyBase.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0015280; F:ligand-gated sodium channel activity; IBA:GO_Central.
DR GO; GO:0005272; F:sodium channel activity; ISS:FlyBase.
DR GO; GO:0015081; F:sodium ion transmembrane transporter activity; IMP:FlyBase.
DR GO; GO:0035002; P:liquid clearance, open tracheal system; IMP:UniProtKB.
DR GO; GO:0035725; P:sodium ion transmembrane transport; IMP:FlyBase.
DR GO; GO:0006814; P:sodium ion transport; ISS:FlyBase.
DR GO; GO:0030104; P:water homeostasis; IMP:FlyBase.
DR InterPro; IPR001873; ENaC.
DR InterPro; IPR020903; ENaC_CS.
DR PANTHER; PTHR11690; PTHR11690; 1.
DR Pfam; PF00858; ASC; 1.
DR PRINTS; PR01078; AMINACHANNEL.
DR PROSITE; PS01206; ASC; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Ion channel; Ion transport; Membrane;
KW Reference proteome; Sodium; Sodium channel; Sodium transport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..535
FT /note="Sodium channel protein Nach"
FT /id="PRO_0000181310"
FT TOPO_DOM 1..49
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 50..70
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 71..471
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 472..492
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 493..535
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 165
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 239
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 367
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 219..237
FT /note="Missing (in isoform A)"
FT /evidence="ECO:0000305"
FT /id="VSP_008551"
FT VAR_SEQ 386
FT /note="F -> FGRLLLICSKYSDFNRLLS (in isoform C)"
FT /evidence="ECO:0000305"
FT /id="VSP_011421"
FT CONFLICT 22
FT /note="Y -> S (in Ref. 1; AAD09149)"
FT /evidence="ECO:0000305"
FT CONFLICT 269
FT /note="S -> L (in Ref. 1; AAD09149)"
FT /evidence="ECO:0000305"
FT CONFLICT 289
FT /note="S -> F (in Ref. 1; AAD09149)"
FT /evidence="ECO:0000305"
FT CONFLICT 312..313
FT /note="SS -> FF (in Ref. 1; AAD09149)"
FT /evidence="ECO:0000305"
FT CONFLICT 365
FT /note="D -> A (in Ref. 1; AAD09149)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 535 AA; 62330 MW; 63C88935FAD62161 CRC64;
MGHQEELKPE QVDLKVTPFV GYLRRTWSDF CATSSIHGLK YTRDEDTNKI VHLVWLLISV
VMFICAVVMA RTFYMDYRSS PTRMNVESDN TPVNRLYFPP VTICPDVLFN MQKSEAFLNT
LRLPKGAELR GILRKLHIFY GFMLDDERYS AEDIEQMEAL LFLNNLTIPE FVEHLRWNCD
EILYRCRFNG EIMDCSKIFQ LSKTFFGHCC SFNLRQKGWV NNKLNNLESF KVFHLNSLNF
TAQRAIGGLR YGLSVVVRYK DDNYDPLQSY SYGVKLLIQE ADAFPSAHSA AKFIAFNSET
FAAVRPQETF CSSAVKALII EERNCVFQNE FPMRYFSDYV YPNCELNCRV TNMVKFCGCH
TYFFDFNRTS DRICTFRDIP CLVDNFANII TRKKSTQCYC PLTCEHIDYD VQLTNFPLEL
NMPVADKFYS GLAKNDGVLH VFINSFSYRR LRRDLLSNMV TLVSNLGSAF SLFVGMSMLS
VVEIIYYFSV ILRKNYKLEC ETRSQMLHKK PKFAWPKAND THSKEQKSVF IIHKS
