NAC_ECOLI
ID NAC_ECOLI Reviewed; 305 AA.
AC Q47005; Q47082;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Nitrogen assimilation regulatory protein nac;
DE AltName: Full=Nitrogen assimilation control protein;
GN Name=nac; OrderedLocusNames=b1988, JW1967;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=K12;
RX PubMed=9495755; DOI=10.1128/jb.180.5.1166-1173.1998;
RA Muse W.B., Bender R.A.;
RT "The nac (nitrogen assimilation control) gene from Escherichia coli.";
RL J. Bacteriol. 180:1166-1173(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 245-305.
RC STRAIN=K12;
RX PubMed=8529872; DOI=10.1016/0378-1119(95)00606-8;
RA Iwanicka-Nowicka R., Hryniewicz M.M.;
RT "A new gene, cbl, encoding a member of the LysR family of transcriptional
RT regulators belongs to Escherichia coli cys regulon.";
RL Gene 166:11-17(1995).
CC -!- FUNCTION: Transcriptional activator for the hut, put and ure operons
CC and repressor for the gdh and gltB operons in response to nitrogen
CC limitation. Negative regulator of its own expression (By similarity).
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC {ECO:0000305}.
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DR EMBL; U56736; AAD14869.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75050.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15806.1; -; Genomic_DNA.
DR EMBL; L31639; AAA99928.1; -; Genomic_DNA.
DR PIR; D64963; D64963.
DR RefSeq; NP_416493.1; NC_000913.3.
DR RefSeq; WP_001011462.1; NZ_SSUV01000031.1.
DR AlphaFoldDB; Q47005; -.
DR SMR; Q47005; -.
DR BioGRID; 4260407; 8.
DR IntAct; Q47005; 1.
DR STRING; 511145.b1988; -.
DR PaxDb; Q47005; -.
DR PRIDE; Q47005; -.
DR DNASU; 946501; -.
DR EnsemblBacteria; AAC75050; AAC75050; b1988.
DR EnsemblBacteria; BAA15806; BAA15806; BAA15806.
DR GeneID; 946501; -.
DR KEGG; ecj:JW1967; -.
DR KEGG; eco:b1988; -.
DR PATRIC; fig|1411691.4.peg.265; -.
DR EchoBASE; EB4013; -.
DR eggNOG; COG0583; Bacteria.
DR HOGENOM; CLU_039613_6_5_6; -.
DR InParanoid; Q47005; -.
DR OMA; QIILRQM; -.
DR PhylomeDB; Q47005; -.
DR BioCyc; EcoCyc:G7072-MON; -.
DR PRO; PR:Q47005; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:EcoCyc.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:EcoCyc.
DR GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:EcoCyc.
DR GO; GO:2000142; P:regulation of DNA-templated transcription, initiation; IBA:GO_Central.
DR Gene3D; 1.10.10.10; -; 1.
DR InterPro; IPR005119; LysR_subst-bd.
DR InterPro; IPR000847; Tscrpt_reg_HTH_LysR.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR Pfam; PF00126; HTH_1; 1.
DR Pfam; PF03466; LysR_substrate; 1.
DR PRINTS; PR00039; HTHLYSR.
DR SUPFAM; SSF46785; SSF46785; 1.
DR PROSITE; PS50931; HTH_LYSR; 1.
PE 3: Inferred from homology;
KW Activator; DNA-binding; Nitrate assimilation; Reference proteome;
KW Repressor; Transcription; Transcription regulation.
FT CHAIN 1..305
FT /note="Nitrogen assimilation regulatory protein nac"
FT /id="PRO_0000105686"
FT DOMAIN 1..58
FT /note="HTH lysR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT DNA_BIND 18..37
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
SQ SEQUENCE 305 AA; 32835 MW; B0B5DC408C6C6821 CRC64;
MNFRRLKYFV KIVDIGSLTQ AAEVLHIAQP ALSQQVATLE GELNQQLLIR TKRGVTPTDA
GKILYTHARA ILRQCEQAQL AVHNVGQALS GQVSIGFAPG TAASSITMPL LQAVRAEFPE
IVIYLHENSG AVLNEKLINH QLDMAVIYEH SPVAGVSSQA LLKEDLFLVG TQDCPGQSVD
VNAIAQMNLF LPSDYSAIRL RVDEAFSLRR LTAKVIGEIE SIATLTAAIA SGMGVAVLPE
SAARSLCGAV NGWMSRITTP SMSLSLSLNL PARANLSPQA QAVKELLMSV ISSPVMEKRQ
WQLVS
