ID   NAC_KLEAE               Reviewed;         305 AA.
AC   Q08597;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Nitrogen assimilation regulatory protein nac;
DE   AltName: Full=Nitrogen assimilation control protein;
GN   Name=nac;
OS   Klebsiella aerogenes (Enterobacter aerogenes).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=548;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=W70 / KC1043;
RX   PubMed=8458853; DOI=10.1128/jb.175.7.2107-2115.1993;
RA   Schwacha A., Bender R.A.;
RT   "The nac (nitrogen assimilation control) gene from Klebsiella aerogenes.";
RL   J. Bacteriol. 175:2107-2115(1993).
CC   -!- FUNCTION: Transcriptional activator for the hut, put and ure operons
CC       and repressor for the gdh and gltB operons in response to nitrogen
CC       limitation. Negative regulator of its own expression.
CC   -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC       {ECO:0000305}.
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DR   EMBL; L01114; AAA18173.1; -; Genomic_DNA.
DR   RefSeq; WP_002911729.1; NZ_LR134254.1.
DR   AlphaFoldDB; Q08597; -.
DR   SMR; Q08597; -.
DR   STRING; 548.EAG7_00767; -.
DR   GeneID; 61333736; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0042128; P:nitrate assimilation; IEA:UniProtKB-KW.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR005119; LysR_subst-bd.
DR   InterPro; IPR000847; Tscrpt_reg_HTH_LysR.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00126; HTH_1; 1.
DR   Pfam; PF03466; LysR_substrate; 1.
DR   PRINTS; PR00039; HTHLYSR.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS50931; HTH_LYSR; 1.
PE   3: Inferred from homology;
KW   Activator; DNA-binding; Nitrate assimilation; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..305
FT                   /note="Nitrogen assimilation regulatory protein nac"
FT                   /id="PRO_0000105687"
FT   DOMAIN          1..58
FT                   /note="HTH lysR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT   DNA_BIND        18..37
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
SQ   SEQUENCE   305 AA;  32754 MW;  775F219AF32AFADC CRC64;
     MNLRRLKYFV KIVDIGSLTQ AAEVLHIAQP ALSQQVATLE GEMDQQLLIR TKRGVTPTEA
     GKILYTHART ILRQCEQAQL AVNNVGQTLR GQVSIGLAPG TAASAITMPL LQTVRNELPE
     VMVYLQESSG TALNDKLLAG QLDMAVLYER SPVAGIVSQP LLKEDLYLVG TRDCPGQSVD
     LTAVAEMNLF LPRDYSAVRA RVTEAFTLRR LSAKIIGEIE SITTLTAAIA SGMGATVLPE
     SAARSLCGAA NGWMARISTP SMSLSLSLNM SARGSLSPQA QAVKEILLSL VSRPSLENRE
     LQLVS