NAD3_CAEEL
ID NAD3_CAEEL Reviewed; 566 AA.
AC Q21339; Q86G93; Q86R85;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 15-MAR-2004, sequence version 3.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Sodium-dependent high-affinity dicarboxylate transporter 3;
DE AltName: Full=Na(+)/dicarboxylate cotransporter 3;
DE Short=NaDC-3;
DE Short=ceNaDC3;
GN Name=nac-3; Synonyms=indy-2, nad-2; ORFNames=K08E5.2;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), FUNCTION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=Bristol N2;
RX PubMed=12480943; DOI=10.1074/jbc.m208763200;
RA Fei Y.-J., Inoue K., Ganapathy V.;
RT "Structural and functional characteristics of two sodium-coupled
RT dicarboxylate transporters (ceNaDC1 and ceNaDC2) from Caenorhabditis
RT elegans and their relevance to life span.";
RL J. Biol. Chem. 278:6136-6144(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: High-affinity sodium-dicarboxylate cotransporter that accepts
CC a range of tricarboxylic acid-cycle intermediates with 4-5 carbon
CC atoms. There is no interaction with monocarboxylates. Plays a role in
CC the regulation of life span. {ECO:0000269|PubMed:12480943}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a;
CC IsoId=Q21339-1; Sequence=Displayed;
CC Name=b;
CC IsoId=Q21339-2; Sequence=VSP_009680;
CC -!- TISSUE SPECIFICITY: Nad-1 and nad-2 are coexpressed in the intestinal
CC tract from early larvae to adults, expression is from the pharynx
CC through to the anus. Expression level is significantly greater in the
CC anterior half of the intestine than in the posterior half.
CC {ECO:0000269|PubMed:12480943}.
CC -!- DEVELOPMENTAL STAGE: Not detected in the embryo. Expressed at the early
CC larval, late larval and adult stages, with highest levels at the late
CC larval stage. {ECO:0000269|PubMed:12480943}.
CC -!- SIMILARITY: Belongs to the SLC13A/DASS transporter (TC 2.A.47) family.
CC NADC subfamily. {ECO:0000305}.
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DR EMBL; AY090485; AAM11894.1; -; mRNA.
DR EMBL; Z30974; CAA83225.3; -; Genomic_DNA.
DR EMBL; Z30974; CAD82916.1; -; Genomic_DNA.
DR PIR; G88575; G88575.
DR RefSeq; NP_001022673.1; NM_001027502.3. [Q21339-1]
DR RefSeq; NP_871713.1; NM_181984.5.
DR AlphaFoldDB; Q21339; -.
DR SMR; Q21339; -.
DR STRING; 6239.K08E5.2a; -.
DR TCDB; 2.A.47.1.8; the divalent anion:na(+) symporter (dass) family.
DR iPTMnet; Q21339; -.
DR EPD; Q21339; -.
DR PaxDb; Q21339; -.
DR EnsemblMetazoa; K08E5.2a.1; K08E5.2a.1; WBGene00003519. [Q21339-1]
DR EnsemblMetazoa; K08E5.2b.1; K08E5.2b.1; WBGene00003519. [Q21339-2]
DR EnsemblMetazoa; K08E5.2b.2; K08E5.2b.2; WBGene00003519. [Q21339-2]
DR GeneID; 176429; -.
DR KEGG; cel:CELE_K08E5.2; -.
DR UCSC; K08E5.2b.2; c. elegans. [Q21339-1]
DR CTD; 176429; -.
DR WormBase; K08E5.2a; CE33531; WBGene00003519; nac-3. [Q21339-1]
DR WormBase; K08E5.2b; CE33532; WBGene00003519; nac-3. [Q21339-2]
DR eggNOG; KOG1281; Eukaryota.
DR GeneTree; ENSGT01030000234550; -.
DR InParanoid; Q21339; -.
DR OMA; YCELAFH; -.
DR OrthoDB; 389981at2759; -.
DR PhylomeDB; Q21339; -.
DR Reactome; R-CEL-433137; Sodium-coupled sulphate, di- and tri-carboxylate transporters.
DR PRO; PR:Q21339; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00003519; Expressed in larva and 3 other tissues.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0015137; F:citrate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015362; F:high-affinity sodium:dicarboxylate symporter activity; IDA:WormBase.
DR GO; GO:0015141; F:succinate transmembrane transporter activity; IDA:WormBase.
DR GO; GO:0098656; P:anion transmembrane transport; IBA:GO_Central.
DR GO; GO:0015746; P:citrate transport; IBA:GO_Central.
DR GO; GO:0008340; P:determination of adult lifespan; IMP:WormBase.
DR GO; GO:0015744; P:succinate transport; IDA:WormBase.
DR InterPro; IPR031312; Na/sul_symport_CS.
DR InterPro; IPR001898; SLC13A/DASS.
DR Pfam; PF00939; Na_sulph_symp; 1.
DR PROSITE; PS01271; NA_SULFATE; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Ion transport; Membrane; Reference proteome; Sodium;
KW Sodium transport; Symport; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..566
FT /note="Sodium-dependent high-affinity dicarboxylate
FT transporter 3"
FT /id="PRO_0000172498"
FT TRANSMEM 55..75
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 92..112
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 123..139
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 162..182
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 219..239
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 268..288
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 329..349
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 352..372
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 400..420
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 439..459
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 496..516
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 521..541
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..88
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_009680"
SQ SEQUENCE 566 AA; 62326 MW; F243921DEC9BC131 CRC64;
MSLSLHLSGW PENSESRQRM LESSSSTNFV QHFVSREISN KERLLSLFRR LRNGLVLVLT
PLFFGQMLNW EGPEWKCAYC VCIIAVYWMS EVMPLAVTAM LPVVLFPLVG VLDANTTAKE
YMNDTNFLFI GGLIMAAAVE KCDLHERVAL SVLRCVGSEP KWIMLGFMTV TALLSSFISN
TATTAMMVPI GQSVVQQLIS SFQHHPTNGE RGRLGCKKMA TGLVLSICFA ANIGGTGTAT
GTPSNLVMLG QLSALFPKVD GSLNYVTWIF FAYPLMLLCL FVAWMTLVSF FLRDAPEKDE
AVTEMLKTRY NELPRMTYAE KSVFVCFCIL LSLWVFRNPG VVPGFGVFFK KGAYTDATSA
MIVAFLLFVL PSERPDLATY IKKEDLKKRG CLMDWKTMQE TFPWSVVLLL GGGFALAAGV
KESGLSLLIG NSLSSIEHLP LWILQLLTML IAMVITNICS NTVTASIFVP IVATLAQRAG
HHPFTLMLPT TLASSFAFIF PVGTPPNAIV FGSGMVKVSD MAFVGGIISL ELLVLTVLYM
NSIAYLTLPL LEFPTWAIIA NSTMQQ
