A16L2_MOUSE
ID A16L2_MOUSE Reviewed; 623 AA.
AC Q6KAU8; D3Z653; G9M4M8; Q6PAU0;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 2.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Protein Atg16l2 {ECO:0000305};
DE AltName: Full=APG16-like 2;
DE AltName: Full=Autophagy-related protein 16-2;
GN Name=Atg16l2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=15449545; DOI=10.1093/dnares/11.2.127;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Kitamura H., Nakagawa T., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of FLJ genes: the
RT complete nucleotide sequences of 110 mouse FLJ-homologous cDNAs identified
RT by screening of terminal sequences of cDNA clones randomly sampled from
RT size-fractionated libraries.";
RL DNA Res. 11:127-135(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 3), TISSUE SPECIFICITY, SUBUNIT,
RP INTERACTION WITH ATG5 AND RAB33B, AND SUBCELLULAR LOCATION.
RX PubMed=22082872; DOI=10.4161/auto.7.12.18025;
RA Ishibashi K., Fujita N., Kanno E., Omori H., Yoshimori T., Itoh T.,
RA Fukuda M.;
RT "Atg16L2, a novel isoform of mammalian Atg16L that is not essential for
RT canonical autophagy despite forming an Atg12-5-16L2 complex.";
RL Autophagy 7:1500-1513(2011).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=24089209; DOI=10.1038/nature12639;
RA Pampliega O., Orhon I., Patel B., Sridhar S., Diaz-Carretero A., Beau I.,
RA Codogno P., Satir B.H., Satir P., Cuervo A.M.;
RT "Functional interaction between autophagy and ciliogenesis.";
RL Nature 502:194-200(2013).
RN [6]
RP DISRUPTION PHENOTYPE, AND FUNCTION.
RX PubMed=31451676; DOI=10.4049/jimmunol.1800419;
RA Khor B., Conway K.L., Omar A.S., Biton M., Haber A.L., Rogel N., Baxt L.A.,
RA Begun J., Kuballa P., Gagnon J.D., Lassen K.G., Regev A., Xavier R.J.;
RT "Distinct Tissue-Specific Roles for the Disease-Associated Autophagy Genes
RT ATG16L2 and ATG16L1.";
RL J. Immunol. 203:1820-1829(2019).
CC -!- FUNCTION: May play a role in regulating epithelial homeostasis in an
CC ATG16L1-dependent manner. {ECO:0000269|PubMed:31451676}.
CC -!- SUBUNIT: Homooligomer (PubMed:22082872). Heterooligomer with ATG16L2
CC (PubMed:22082872). Interacts with ATG5 (PubMed:22082872). Self-
CC oligomerizes to form a 800-kDa complex composed of ATG12-ATG5 and
CC ATG16L2 (PubMed:22082872). Interacts with RAB33B (PubMed:22082872).
CC {ECO:0000269|PubMed:22082872}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:22082872}.
CC Note=Localizes also to discrete punctae along the ciliary axoneme.
CC {ECO:0000269|PubMed:24089209}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1; Synonyms=Atg16L2 beta {ECO:0000303|PubMed:22082872};
CC IsoId=Q6KAU8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q6KAU8-2; Sequence=VSP_033906, VSP_033907;
CC Name=3; Synonyms=Atg16L2 alpha {ECO:0000303|PubMed:22082872};
CC IsoId=Q6KAU8-3; Sequence=VSP_060643;
CC -!- TISSUE SPECIFICITY: Widely expressed. {ECO:0000269|PubMed:22082872}.
CC -!- DISRUPTION PHENOTYPE: ATG16L2-deficient mice are viable and born at
CC Mendelian proportions. Deficient mice exhibit intact canonical
CC autophagy. {ECO:0000269|PubMed:31451676}.
CC -!- MISCELLANEOUS: Although ATG16L2 is structurally similar to ATG16L1 and
CC is likewise able to form a complex with the autophagy proteins ATG5 and
CC ATG12, overexpression and knockdown studies suggest that ATG16L2 is not
CC essential for canonical autophagy. {ECO:0000269|PubMed:22082872,
CC ECO:0000269|PubMed:31451676}.
CC -!- SIMILARITY: Belongs to the WD repeat ATG16 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH60054.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAD21359.1; Type=Miscellaneous discrepancy; Note=The sequence differs from that shown because it seems to be derived from a pre-mRNA.; Evidence={ECO:0000305};
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DR EMBL; AK131109; BAD21359.1; ALT_SEQ; Transcribed_RNA.
DR EMBL; AC107638; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC060054; AAH60054.1; ALT_INIT; mRNA.
DR EMBL; AB476648; BAL42360.1; -; mRNA.
DR EMBL; AB476647; BAL42359.1; -; mRNA.
DR CCDS; CCDS52329.1; -. [Q6KAU8-1]
DR RefSeq; NP_001104581.1; NM_001111111.1. [Q6KAU8-1]
DR AlphaFoldDB; Q6KAU8; -.
DR SMR; Q6KAU8; -.
DR BioGRID; 216189; 2.
DR ComplexPortal; CPX-355; Atg12-Atg5-Atg16l2 complex.
DR STRING; 10090.ENSMUSP00000112500; -.
DR iPTMnet; Q6KAU8; -.
DR PhosphoSitePlus; Q6KAU8; -.
DR EPD; Q6KAU8; -.
DR jPOST; Q6KAU8; -.
DR MaxQB; Q6KAU8; -.
DR PaxDb; Q6KAU8; -.
DR PRIDE; Q6KAU8; -.
DR ProteomicsDB; 286033; -. [Q6KAU8-1]
DR ProteomicsDB; 286034; -. [Q6KAU8-2]
DR ProteomicsDB; 335064; -.
DR Antibodypedia; 30896; 198 antibodies from 26 providers.
DR Ensembl; ENSMUST00000120267; ENSMUSP00000112500; ENSMUSG00000047767. [Q6KAU8-1]
DR Ensembl; ENSMUST00000122116; ENSMUSP00000113320; ENSMUSG00000047767. [Q6KAU8-3]
DR Ensembl; ENSMUST00000139609; ENSMUSP00000117387; ENSMUSG00000047767. [Q6KAU8-2]
DR Ensembl; ENSMUST00000143630; ENSMUSP00000117029; ENSMUSG00000047767. [Q6KAU8-2]
DR GeneID; 73683; -.
DR KEGG; mmu:73683; -.
DR UCSC; uc009iof.2; mouse. [Q6KAU8-1]
DR UCSC; uc009iog.2; mouse.
DR CTD; 89849; -.
DR MGI; MGI:1920933; Atg16l2.
DR VEuPathDB; HostDB:ENSMUSG00000047767; -.
DR eggNOG; KOG0288; Eukaryota.
DR GeneTree; ENSGT00940000153936; -.
DR HOGENOM; CLU_000288_57_10_1; -.
DR InParanoid; Q6KAU8; -.
DR OMA; PHCAAIN; -.
DR OrthoDB; 404224at2759; -.
DR PhylomeDB; Q6KAU8; -.
DR TreeFam; TF315541; -.
DR BioGRID-ORCS; 73683; 3 hits in 74 CRISPR screens.
DR ChiTaRS; Atg16l2; mouse.
DR PRO; PR:Q6KAU8; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q6KAU8; protein.
DR Bgee; ENSMUSG00000047767; Expressed in granulocyte and 151 other tissues.
DR ExpressionAtlas; Q6KAU8; baseline and differential.
DR Genevisible; Q6KAU8; MM.
DR GO; GO:0034274; C:Atg12-Atg5-Atg16 complex; IDA:ComplexPortal.
DR GO; GO:0000421; C:autophagosome membrane; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0034045; C:phagophore assembly site membrane; IDA:ComplexPortal.
DR GO; GO:0000045; P:autophagosome assembly; IMP:ComplexPortal.
DR GO; GO:0016236; P:macroautophagy; IMP:ComplexPortal.
DR GO; GO:0039689; P:negative stranded viral RNA replication; IMP:MGI.
DR GO; GO:0006497; P:protein lipidation; ISO:MGI.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR045160; ATG16.
DR InterPro; IPR013923; Autophagy-rel_prot_16_dom.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR19878; PTHR19878; 1.
DR Pfam; PF08614; ATG16; 1.
DR Pfam; PF00400; WD40; 2.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 3.
DR PROSITE; PS50082; WD_REPEATS_2; 4.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Cytoplasm; Protein transport;
KW Reference proteome; Repeat; Transport; WD repeat.
FT CHAIN 1..623
FT /note="Protein Atg16l2"
FT /id="PRO_0000337111"
FT REPEAT 338..377
FT /note="WD 1"
FT /evidence="ECO:0000255"
FT REPEAT 382..421
FT /note="WD 2"
FT /evidence="ECO:0000255"
FT REPEAT 424..458
FT /note="WD 3"
FT /evidence="ECO:0000255"
FT REPEAT 459..502
FT /note="WD 4"
FT /evidence="ECO:0000255"
FT REPEAT 504..543
FT /note="WD 5"
FT /evidence="ECO:0000255"
FT REPEAT 550..589
FT /note="WD 6"
FT /evidence="ECO:0000255"
FT REPEAT 593..623
FT /note="WD 7"
FT /evidence="ECO:0000255"
FT REGION 64..93
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 116..229
FT /evidence="ECO:0000255"
FT COMPBIAS 64..80
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 279..300
FT /note="RSASATSLTLSRCVDVVKGLLD -> S (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:22082872"
FT /id="VSP_060643"
FT VAR_SEQ 396..403
FT /note="GSQVLAAT -> VRRLCPTA (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_033906"
FT VAR_SEQ 404..623
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_033907"
SQ SEQUENCE 623 AA; 69241 MW; 0358F9B735BC44D6 CRC64;
MAGPGAPCDP CAPAAVWKRH IVRQLRHRDR TQKALFLELV PAYNHLLEKA ELLAKFSEKL
KSEPKDAIST RHEDWREEVS GTGPDQVSSP ASLRVKWQQE KKGLQLVCGE MAYQVVKKSA
ALDTLQSQLE ERQDRLEALQ ACVVQLQEAR AQQSRQLEER QAENAAQREA YETLLQQAVH
QEAALRRLQE EARDLLEQLV QRKARAAAER NLRNERRERA NQALVSQELK KAAKRTVSIS
EIPNTLEDGT KEETVALAPA ALPEFSESET CEKWKRPFRS ASATSLTLSR CVDVVKGLLD
FKKRRGHSVG GAPEQRYQSI PVCVSAQIPS QAQDVLDAHL SEVNAVCFGP NSSLLATGGA
DRLIHLWNVV GGRLEANQTL EGAGGSITSV DFDPSGSQVL AATYNQAAQL WKVGETQSKE
TLSGHKDKVT AAKFKLTRHQ AVTGSRDRTV KEWDLGRAYC SRTINVLSYC NDVVCGDHII
ISGHNDQKIR FWDSRGPHCI QVIPVQGRVT SLHLSYDQLH LLSCSRDNTL KVIDLRISNI
RQVFRADGFK CSSDWTKAVF SPDRSYALAG SSNGDLYIWD VNTGKLETSL QGPHCTAVNA
VAWCFSGNHV VSVDQGRKVV LWH
