NADD_MYCTU
ID NADD_MYCTU Reviewed; 214 AA.
AC P9WJJ5; L0TCA5; O86328;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 25-MAY-2022, sequence version 2.
DT 03-AUG-2022, entry version 44.
DE RecName: Full=Probable nicotinate-nucleotide adenylyltransferase {ECO:0000255|HAMAP-Rule:MF_00244};
DE EC=2.7.7.18 {ECO:0000255|HAMAP-Rule:MF_00244};
DE AltName: Full=Deamido-NAD(+) diphosphorylase {ECO:0000255|HAMAP-Rule:MF_00244};
DE AltName: Full=Deamido-NAD(+) pyrophosphorylase {ECO:0000255|HAMAP-Rule:MF_00244};
DE AltName: Full=Nicotinate mononucleotide adenylyltransferase {ECO:0000255|HAMAP-Rule:MF_00244};
DE Short=NaMN adenylyltransferase {ECO:0000255|HAMAP-Rule:MF_00244};
GN Name=nadD {ECO:0000255|HAMAP-Rule:MF_00244}; OrderedLocusNames=Rv2421c;
GN ORFNames=MTCY428.26;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP PROTEIN SEQUENCE OF 1-42, AND SEQUENCE REVISION TO N-TERMINUS.
RC STRAIN=H37Rv;
RX PubMed=34915127; DOI=10.1016/j.ygeno.2021.12.001;
RA Shi J., Meng S., Wan L., Zhang Z., Jiang S., Zhu H., Dai E., Chang L.,
RA Gao H., Wan K., Zhang L., Zhao X., Liu H., Lyu Z., Zhang Y., Xu P.;
RT "Deep N-terminomics of Mycobacterium tuberculosis H37Rv extensively correct
RT annotated encoding genes.";
RL Genomics 114:292-304(2022).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- FUNCTION: Catalyzes the reversible adenylation of nicotinate
CC mononucleotide (NaMN) to nicotinic acid adenine dinucleotide (NaAD).
CC {ECO:0000255|HAMAP-Rule:MF_00244}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H(+) + nicotinate beta-D-ribonucleotide = deamido-NAD(+)
CC + diphosphate; Xref=Rhea:RHEA:22860, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57502,
CC ChEBI:CHEBI:58437; EC=2.7.7.18; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00244};
CC -!- PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis; deamido-NAD(+)
CC from nicotinate D-ribonucleotide: step 1/1. {ECO:0000255|HAMAP-
CC Rule:MF_00244}.
CC -!- SIMILARITY: Belongs to the NadD family. {ECO:0000255|HAMAP-
CC Rule:MF_00244, ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CCP45212.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000269|PubMed:34915127};
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DR EMBL; AL123456; CCP45212.1; ALT_INIT; Genomic_DNA.
DR PIR; H70685; H70685.
DR RefSeq; NP_216937.1; NC_000962.3.
DR RefSeq; WP_003412398.1; NZ_NVQJ01000054.1.
DR PDB; 4RPI; X-ray; 2.42 A; A/B/C/D=4-214.
DR PDB; 4S1O; X-ray; 1.84 A; A/B=4-195.
DR PDB; 4X0E; X-ray; 2.41 A; A/B=4-214.
DR PDB; 4YBR; X-ray; 1.65 A; A/B=4-195.
DR PDB; 5DAS; X-ray; 2.20 A; A/B/C/D=4-195.
DR PDB; 6BUV; X-ray; 1.86 A; A/B=4-195.
DR PDBsum; 4RPI; -.
DR PDBsum; 4S1O; -.
DR PDBsum; 4X0E; -.
DR PDBsum; 4YBR; -.
DR PDBsum; 5DAS; -.
DR PDBsum; 6BUV; -.
DR AlphaFoldDB; P9WJJ5; -.
DR SMR; P9WJJ5; -.
DR STRING; 83332.Rv2421c; -.
DR PaxDb; P9WJJ5; -.
DR DNASU; 885457; -.
DR GeneID; 885457; -.
DR KEGG; mtu:Rv2421c; -.
DR TubercuList; Rv2421c; -.
DR eggNOG; COG1057; Bacteria.
DR OMA; IHIGHLI; -.
DR PhylomeDB; P9WJJ5; -.
DR BRENDA; 2.7.7.18; 3445.
DR UniPathway; UPA00253; UER00332.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0000309; F:nicotinamide-nucleotide adenylyltransferase activity; IBA:GO_Central.
DR GO; GO:0004515; F:nicotinate-nucleotide adenylyltransferase activity; IBA:GO_Central.
DR GO; GO:0009435; P:NAD biosynthetic process; IBA:GO_Central.
DR CDD; cd02165; NMNAT; 1.
DR Gene3D; 3.40.50.620; -; 1.
DR HAMAP; MF_00244; NaMN_adenylyltr; 1.
DR InterPro; IPR004821; Cyt_trans-like.
DR InterPro; IPR005248; NadD/NMNAT.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR Pfam; PF01467; CTP_transf_like; 1.
DR TIGRFAMs; TIGR00125; cyt_tran_rel; 1.
DR TIGRFAMs; TIGR00482; TIGR00482; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP-binding; Direct protein sequencing; NAD;
KW Nucleotide-binding; Nucleotidyltransferase;
KW Pyridine nucleotide biosynthesis; Reference proteome; Transferase.
FT CHAIN 1..214
FT /note="Probable nicotinate-nucleotide adenylyltransferase"
FT /id="PRO_0000181427"
FT STRAND 1..3
FT /evidence="ECO:0007829|PDB:4YBR"
FT HELIX 10..22
FT /evidence="ECO:0007829|PDB:4YBR"
FT STRAND 26..32
FT /evidence="ECO:0007829|PDB:4YBR"
FT STRAND 37..39
FT /evidence="ECO:0007829|PDB:6BUV"
FT HELIX 46..57
FT /evidence="ECO:0007829|PDB:4YBR"
FT STRAND 63..65
FT /evidence="ECO:0007829|PDB:4YBR"
FT HELIX 68..72
FT /evidence="ECO:0007829|PDB:4YBR"
FT STRAND 73..75
FT /evidence="ECO:0007829|PDB:4S1O"
FT HELIX 78..88
FT /evidence="ECO:0007829|PDB:4YBR"
FT STRAND 92..99
FT /evidence="ECO:0007829|PDB:4YBR"
FT HELIX 100..109
FT /evidence="ECO:0007829|PDB:4YBR"
FT HELIX 112..118
FT /evidence="ECO:0007829|PDB:4YBR"
FT STRAND 120..126
FT /evidence="ECO:0007829|PDB:4YBR"
FT HELIX 129..132
FT /evidence="ECO:0007829|PDB:4YBR"
FT HELIX 134..144
FT /evidence="ECO:0007829|PDB:4YBR"
FT HELIX 145..147
FT /evidence="ECO:0007829|PDB:4YBR"
FT STRAND 148..152
FT /evidence="ECO:0007829|PDB:4YBR"
FT HELIX 154..157
FT /evidence="ECO:0007829|PDB:4YBR"
FT HELIX 160..168
FT /evidence="ECO:0007829|PDB:4YBR"
FT HELIX 179..187
FT /evidence="ECO:0007829|PDB:4YBR"
FT TURN 188..191
FT /evidence="ECO:0007829|PDB:4YBR"
SQ SEQUENCE 214 AA; 23340 MW; D4F2D71B86F30385 CRC64;
MGVMGGTFDP IHYGHLVAAS EVADLFDLDE VVFVPSGQPW QKGRQVSAAE HRYLMTVIAT
ASNPRFSVSR VDIDRGGPTY TKDTLADLHA LHPDSELYFT TGADALASIM SWQGWEELFE
LARFVGVSRP GYELRNEHIT SLLGQLAKDA LTLVEIPALA ISSTDCRQRA EQSRPLWYLM
PDGVVQYVSK CRLYCGACDA GARSTTSLAA GNGL
