NADE_ORYSJ
ID NADE_ORYSJ Reviewed; 735 AA.
AC Q0D8D4;
DT 18-SEP-2013, integrated into UniProtKB/Swiss-Prot.
DT 18-SEP-2013, sequence version 2.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Glutamine-dependent NAD(+) synthetase;
DE EC=6.3.5.1;
DE AltName: Full=NAD(+) synthase [glutamine-hydrolyzing];
DE AltName: Full=NAD(+) synthetase;
GN OrderedLocusNames=Os07g0167100, LOC_Os07g07260;
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + deamido-NAD(+) + H2O + L-glutamine = AMP + diphosphate +
CC H(+) + L-glutamate + NAD(+); Xref=Rhea:RHEA:24384, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:57540, ChEBI:CHEBI:58359,
CC ChEBI:CHEBI:58437, ChEBI:CHEBI:456215; EC=6.3.5.1;
CC -!- PATHWAY: Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from
CC deamido-NAD(+) (L-Gln route): step 1/1.
CC -!- SIMILARITY: In the C-terminal section; belongs to the NAD synthetase
CC family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAF20889.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP008213; BAF20889.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014963; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_015646532.1; XM_015791046.1.
DR AlphaFoldDB; Q0D8D4; -.
DR SMR; Q0D8D4; -.
DR STRING; 4530.OS07T0167100-01; -.
DR PaxDb; Q0D8D4; -.
DR PRIDE; Q0D8D4; -.
DR GeneID; 4342490; -.
DR KEGG; osa:4342490; -.
DR eggNOG; KOG2303; Eukaryota.
DR HOGENOM; CLU_011884_3_0_1; -.
DR InParanoid; Q0D8D4; -.
DR OrthoDB; 283044at2759; -.
DR UniPathway; UPA00253; UER00334.
DR Proteomes; UP000000763; Chromosome 7.
DR Proteomes; UP000059680; Chromosome 7.
DR Genevisible; Q0D8D4; OS.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004359; F:glutaminase activity; IBA:GO_Central.
DR GO; GO:0003952; F:NAD+ synthase (glutamine-hydrolyzing) activity; IBA:GO_Central.
DR GO; GO:0009435; P:NAD biosynthetic process; IBA:GO_Central.
DR CDD; cd00553; NAD_synthase; 1.
DR Gene3D; 3.40.50.620; -; 1.
DR Gene3D; 3.60.110.10; -; 1.
DR HAMAP; MF_02090; NadE_glutamine_dep; 1.
DR InterPro; IPR003010; C-N_Hydrolase.
DR InterPro; IPR036526; C-N_Hydrolase_sf.
DR InterPro; IPR014445; Gln-dep_NAD_synthase.
DR InterPro; IPR022310; NAD/GMP_synthase.
DR InterPro; IPR003694; NAD_synthase.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR23090; PTHR23090; 1.
DR Pfam; PF00795; CN_hydrolase; 1.
DR Pfam; PF02540; NAD_synthase; 1.
DR PIRSF; PIRSF006630; NADS_GAT; 1.
DR SUPFAM; SSF56317; SSF56317; 1.
DR TIGRFAMs; TIGR00552; nadE; 1.
DR PROSITE; PS50263; CN_HYDROLASE; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; NAD; Nucleotide-binding; Reference proteome.
FT CHAIN 1..735
FT /note="Glutamine-dependent NAD(+) synthetase"
FT /id="PRO_0000423485"
FT DOMAIN 4..274
FT /note="CN hydrolase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00054"
FT REGION 324..711
FT /note="Ligase"
FT /evidence="ECO:0000250"
FT ACT_SITE 44
FT /note="Proton acceptor; for glutaminase activity"
FT /evidence="ECO:0000250|UniProtKB:P9WJJ3"
FT ACT_SITE 113
FT /note="For glutaminase activity"
FT /evidence="ECO:0000250|UniProtKB:P9WJJ3"
FT ACT_SITE 174
FT /note="Nucleophile; for glutaminase activity"
FT /evidence="ECO:0000250|UniProtKB:P9WJJ3"
FT ACT_SITE 356
FT /evidence="ECO:0000250"
FT BINDING 354..361
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
SQ SEQUENCE 735 AA; 82077 MW; 96E5E2A198C0F1F5 CRC64;
MRLLRVATCN LNQWAMDFDT NLRNVKESIA RAKAAGAAVR VGPELELTGY GCEDHFLEQD
TAAHAWECLK DILSGGYTDG ILCSIGMPVI FKSVRYNCQV FCLNSKIVMI RPKISLANDG
NYREFRWFSA WTFKDALVDF QLPLDISEVT SQDTVPFGYG FIQFLDVSLA SETCEELFTA
NAPRIDLALN GVEVFVNASG SHHQLRKLSL RIDSMRNATL ACGGVYMYAN QQGCDGGRLY
YDGCCCIAVN GDVVAQGSQF SLKDVEVLDA LVDLDAVSSY RASVSSFREQ ASHRTKVPFV
KVPYKLCKPF QSGMVPTGPV EVMYHRPEEE IAFGPSCWLW DYLRRSRASG FLLPLSGGAD
SSSVAAIVGC MCQLVVKDIE NGDEQVKADA MRIGQYKDGE FPKDSRELAK RLFYTVYMGT
ENSSEGTRSR AKMLAEEIGS FHLDVPIDSI VSALLSLFER LTGKRPRYKV DGGSNTENLG
LQNIQARIRM VLAFMMASLM PWVHNKSGFY LVLGSSNVDE GLRGYLTKYD CSSADINPIG
SVSKQDLRAF LRWAAVHLHY SSLAEVEAAP PTAELEPIRA DYNQLDEVDM GMTYEELSIY
GRLRKIFRCG PVSMFQNLCH RWCGTLSPSE VADKVKHFFK YYAINRHKMT VLTPSYHAES
YSPEDNRFDL RQFLYNARWP YQFRKIDELV QDMDKDGKWV NSTEGELRRR KGVRSAEGGG
MGVVAVGSAN PSAGS
