ID   NADO2_ORYSJ             Reviewed;         322 AA.
AC   Q7G765; A0A0P0XR03; B7EV58; Q0IZA7; Q94LH8;
DT   16-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   03-AUG-2022, entry version 102.
DE   RecName: Full=Probable NAD(P)H-dependent oxidoreductase 2;
DE            EC=1.-.-.-;
GN   OrderedLocusNames=Os10g0113100, LOC_Os10g02380, LOC_Os10g02390;
GN   ORFNames=OJ1014H12.12, OSJNBb0012A20.19;
OS   Oryza sativa subsp. japonica (Rice).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX   NCBI_TaxID=39947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12791992; DOI=10.1126/science.1083523;
RA   Yu Y., Rambo T., Currie J., Saski C., Kim H.-R., Collura K., Thompson S.,
RA   Simmons J., Yang T.-J., Nah G., Patel A.J., Thurmond S., Henry D.,
RA   Oates R., Palmer M., Pries G., Gibson J., Anderson H., Paradkar M.,
RA   Crane L., Dale J., Carver M.B., Wood T., Frisch D., Engler F.,
RA   Soderlund C., Palmer L.E., Teytelman L., Nascimento L., De la Bastide M.,
RA   Spiegel L., Ware D., O'Shaughnessy A., Dike S., Dedhia N., Preston R.,
RA   Huang E., Ferraro K., Kuit K., Miller B., Zutavern T., Katzenberger F.,
RA   Muller S., Balija V., Martienssen R.A., Stein L., Minx P., Johnson D.,
RA   Cordum H., Mardis E., Cheng Z., Jiang J., Wilson R., McCombie W.R.,
RA   Wing R.A., Yuan Q., Ouyang S., Liu J., Jones K.M., Gansberger K.,
RA   Moffat K., Hill J., Tsitrin T., Overton L., Bera J., Kim M., Jin S.,
RA   Tallon L., Ciecko A., Pai G., Van Aken S., Utterback T., Reidmuller S.,
RA   Bormann J., Feldblyum T., Hsiao J., Zismann V., Blunt S., de Vazeille A.R.,
RA   Shaffer T., Koo H., Suh B., Yang Q., Haas B., Peterson J., Pertea M.,
RA   Volfovsky N., Wortman J., White O., Salzberg S.L., Fraser C.M., Buell C.R.,
RA   Messing J., Song R., Fuks G., Llaca V., Kovchak S., Young S., Bowers J.E.,
RA   Paterson A.H., Johns M.A., Mao L., Pan H., Dean R.A.;
RT   "In-depth view of structure, activity, and evolution of rice chromosome
RT   10.";
RL   Science 300:1566-1569(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=16100779; DOI=10.1038/nature03895;
RG   International rice genome sequencing project (IRGSP);
RT   "The map-based sequence of the rice genome.";
RL   Nature 436:793-800(2005).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=18089549; DOI=10.1093/nar/gkm978;
RG   The rice annotation project (RAP);
RT   "The rice annotation project database (RAP-DB): 2008 update.";
RL   Nucleic Acids Res. 36:D1028-D1033(2008).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Nipponbare;
RX   PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA   Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA   Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA   Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA   Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT   "Improvement of the Oryza sativa Nipponbare reference genome using next
RT   generation sequence and optical map data.";
RL   Rice 6:4-4(2013).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Nipponbare;
RX   PubMed=12869764; DOI=10.1126/science.1081288;
RG   The rice full-length cDNA consortium;
RT   "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT   japonica rice.";
RL   Science 301:376-379(2003).
CC   -!- FUNCTION: May play a role in auxin-induced cell growth by generating
CC       hydroxyl radicals, which tends to increase cell wall loosening.
CC       {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the aldo/keto reductase family. {ECO:0000305}.
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DR   EMBL; AC079685; AAK52588.1; -; Genomic_DNA.
DR   EMBL; AC098694; AAM44873.1; -; Genomic_DNA.
DR   EMBL; DP000086; AAP51851.1; -; Genomic_DNA.
DR   EMBL; AP008216; BAF25958.1; -; Genomic_DNA.
DR   EMBL; AP014966; BAT09645.1; -; Genomic_DNA.
DR   EMBL; AK103772; BAG96255.1; -; mRNA.
DR   RefSeq; XP_015613877.1; XM_015758391.1.
DR   AlphaFoldDB; Q7G765; -.
DR   SMR; Q7G765; -.
DR   STRING; 4530.OS10T0113100-01; -.
DR   PRIDE; Q7G765; -.
DR   EnsemblPlants; Os10t0113100-01; Os10t0113100-01; Os10g0113100.
DR   GeneID; 4347978; -.
DR   Gramene; Os10t0113100-01; Os10t0113100-01; Os10g0113100.
DR   KEGG; osa:4347978; -.
DR   eggNOG; KOG1577; Eukaryota.
DR   InParanoid; Q7G765; -.
DR   OMA; PWCMRQE; -.
DR   OrthoDB; 1016440at2759; -.
DR   Proteomes; UP000000763; Chromosome 10.
DR   Proteomes; UP000059680; Chromosome 10.
DR   ExpressionAtlas; Q7G765; baseline and differential.
DR   Genevisible; Q7G765; OS.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0004032; F:alditol:NADP+ 1-oxidoreductase activity; IBA:GO_Central.
DR   CDD; cd19124; AKR_AKR4A_4B; 1.
DR   Gene3D; 3.20.20.100; -; 1.
DR   InterPro; IPR020471; AKR.
DR   InterPro; IPR044497; AKR4A/B.
DR   InterPro; IPR018170; Aldo/ket_reductase_CS.
DR   InterPro; IPR023210; NADP_OxRdtase_dom.
DR   InterPro; IPR036812; NADP_OxRdtase_dom_sf.
DR   Pfam; PF00248; Aldo_ket_red; 1.
DR   PIRSF; PIRSF000097; AKR; 1.
DR   PRINTS; PR00069; ALDKETRDTASE.
DR   SUPFAM; SSF51430; SSF51430; 1.
DR   PROSITE; PS00798; ALDOKETO_REDUCTASE_1; 1.
DR   PROSITE; PS00062; ALDOKETO_REDUCTASE_2; 1.
DR   PROSITE; PS00063; ALDOKETO_REDUCTASE_3; 1.
PE   2: Evidence at transcript level;
KW   NAD; NADP; Oxidoreductase; Reference proteome.
FT   CHAIN           1..322
FT                   /note="Probable NAD(P)H-dependent oxidoreductase 2"
FT                   /id="PRO_0000124656"
FT   ACT_SITE        57
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         120
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         216..275
FT                   /ligand="NADP(+)"
FT                   /ligand_id="ChEBI:CHEBI:58349"
FT                   /evidence="ECO:0000250"
FT   SITE            87
FT                   /note="Lowers pKa of active site Tyr"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   322 AA;  35784 MW;  0B2EA244B66742C9 CRC64;
     MAMATIPEVP ASALLPTMPR IGMGTAAFPF TSSEETTAAL LRAIELGYRH FDTARLYATE
     GCVSEAVAEA VRRGLVASRA DVFVTSKLWC SDLHAGRVVP AARETLRNLG MDYVDLLLVH
     WPATVAPGSY DFPFPKEEMA PAFDMEGVWR GMEECHRLGL ARAIGVSNFS AKKLEQLLSF
     AVVRPAANQV EMNPMWQQRT LREVCRREGV QLCGYSPLGA KGTPWGSAAV MDSGVLHDIA
     QTKGKTLAQI CLRWMYEQGD VLLVKTYNEN RMKENLDIFD WELTEEERDK ISKLPQQRGL
     TGMQFVCDNG PYKCVEDLWD GA