ID   NAG1_YEAST              Reviewed;         163 AA.
AC   Q8TGN9; D6VUG7;
DT   11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2002, sequence version 1.
DT   03-AUG-2022, entry version 74.
DE   RecName: Full=Protein NAG1;
DE   AltName: Full=Nested antisense gene 1 protein;
GN   Name=NAG1; OrderedLocusNames=YGR031C-A;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=SK1;
RX   PubMed=16273108; DOI=10.1038/ng1674;
RA   Deutschbauer A.M., Davis R.W.;
RT   "Quantitative trait loci mapped to single-nucleotide resolution in yeast.";
RL   Nat. Genet. 37:1333-1340(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=9169869;
RA   Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA   Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA   Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA   Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA   Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA   Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA   Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA   Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA   Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA   Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA   Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA   Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA   Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA   Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA   Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA   Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA   Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA   Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA   Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA   Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA   Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA   Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT   "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL   Nature 387:81-84(1997).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=11753363; DOI=10.1038/nbt0102-58;
RA   Kumar A., Harrison P.M., Cheung K.-H., Lan N., Echols N., Bertone P.,
RA   Miller P., Gerstein M.B., Snyder M.;
RT   "An integrated approach for finding overlooked genes in yeast.";
RL   Nat. Biotechnol. 20:58-63(2002).
RN   [5]
RP   IDENTIFICATION, FUNCTION, SUBCELLULAR LOCATION, INDUCTION, AND DISRUPTION
RP   PHENOTYPE.
RX   PubMed=18310357; DOI=10.1128/ec.00053-08;
RA   Ma J., Dobry C.J., Krysan D.J., Kumar A.;
RT   "Unconventional genomic architecture in the budding yeast Saccharomyces
RT   cerevisiae masks the nested antisense gene NAG1.";
RL   Eukaryot. Cell 7:1289-1298(2008).
CC   -!- FUNCTION: Involved in yeast cell wall biogenesis.
CC       {ECO:0000269|PubMed:18310357}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass membrane
CC       protein {ECO:0000305}. Note=Localizes to the cell periphery under
CC       conditions of vegetative growth. {ECO:0000269|PubMed:18310357}.
CC   -!- INDUCTION: Expression is dependent upon the presence of the cell wall
CC       integrity pathway mitogen-activated protein kinase SLT2 and its
CC       downstream transcription factor RLM1. {ECO:0000269|PubMed:18310357}.
CC   -!- DISRUPTION PHENOTYPE: Hypersensitive to cell wall-perturbing agent
CC       calcofluor white (CFW) at 37 degrees Celsius. A similar phenotype is
CC       observed at 30 degrees Celsius, although the severity is decreased.
CC       Decreased expression of genes contributing to cell wall organization
CC       during vegetative growth. {ECO:0000269|PubMed:18310357}.
CC   -!- CAUTION: Overlaps completely with ORF YGR031W. {ECO:0000305}.
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DR   EMBL; DQ115390; AAZ22446.1; -; Genomic_DNA.
DR   EMBL; Z72816; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; AF479950; AAL79263.1; -; Genomic_DNA.
DR   EMBL; BK006941; DAA08128.1; -; Genomic_DNA.
DR   RefSeq; NP_001027023.1; NM_001184658.1.
DR   AlphaFoldDB; Q8TGN9; -.
DR   STRING; 4932.YGR031C-A; -.
DR   PaxDb; Q8TGN9; -.
DR   PRIDE; Q8TGN9; -.
DR   EnsemblFungi; YGR031C-A_mRNA; YGR031C-A; YGR031C-A.
DR   GeneID; 8656591; -.
DR   KEGG; sce:YGR031C-A; -.
DR   SGD; S000028636; NAG1.
DR   VEuPathDB; FungiDB:YGR031C-A; -.
DR   HOGENOM; CLU_1628025_0_0_1; -.
DR   BioCyc; YEAST:G3O-31015-MON; -.
DR   PRO; PR:Q8TGN9; -.
DR   Proteomes; UP000002311; Chromosome VII.
DR   RNAct; Q8TGN9; protein.
DR   GO; GO:0071944; C:cell periphery; HDA:SGD.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR   GO; GO:0042546; P:cell wall biogenesis; IMP:SGD.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
PE   2: Evidence at transcript level;
KW   Cell wall biogenesis/degradation; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..163
FT                   /note="Protein NAG1"
FT                   /id="PRO_0000299922"
FT   TRANSMEM        76..96
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   163 AA;  17536 MW;  CEDDE53B47160CF8 CRC64;
     MNSAGRVHRS RAGSRGHAAI SPLTMASFSV ARGIRSSNVY DDTDDELSIL TFFSAVRRNR
     LTSSLPPILS ARCSSACFSV RIVLPLSLTI SISALMYSTN SALGRKLTGA FSIQTNIEQS
     CGFFRTSIMA TLPPIECPII IGPPLVFNSC FVIKCFTSSD MTS