NAGAB_RALSP
ID NAGAB_RALSP Reviewed; 104 AA.
AC O52381;
DT 06-MAR-2013, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 67.
DE RecName: Full=Naphthalene 1,2-dioxygenase/salicylate 5-hydroxylase systems, ferredoxin component {ECO:0000303|PubMed:11872705};
DE Short=NDO/S5H systems ferredoxin component {ECO:0000303|PubMed:11872705};
DE AltName: Full=Ferredoxin NagAb {ECO:0000303|PubMed:9573207};
GN Name=nagAb {ECO:0000303|PubMed:9573207};
OS Ralstonia sp.
OG Plasmid pWWU2 {ECO:0000312|EMBL:AAD12609.1}.
OC Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC Burkholderiaceae; Ralstonia.
OX NCBI_TaxID=54061;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, PATHWAY, DISRUPTION PHENOTYPE,
RP AND SUBUNIT.
RC STRAIN=U2 {ECO:0000312|EMBL:AAD12609.1};
RX PubMed=9573207; DOI=10.1128/jb.180.9.2522-2530.1998;
RA Fuenmayor S.L., Wild M., Boyes A.L., Williams P.A.;
RT "A gene cluster encoding steps in conversion of naphthalene to gentisate in
RT Pseudomonas sp. strain U2.";
RL J. Bacteriol. 180:2522-2530(1998).
RN [2]
RP FUNCTION, PATHWAY, AND SUBUNIT.
RC STRAIN=U2;
RX PubMed=11872705; DOI=10.1128/jb.184.6.1547-1555.2002;
RA Zhou N.Y., Al-Dulayymi J., Baird M.S., Williams P.A.;
RT "Salicylate 5-hydroxylase from Ralstonia sp. strain U2: a monooxygenase
RT with close relationships to and shared electron transport proteins with
RT naphthalene dioxygenase.";
RL J. Bacteriol. 184:1547-1555(2002).
CC -!- FUNCTION: Component of two multicomponent enzyme systems which are
CC involved in the catabolism of naphthalene (PubMed:11872705,
CC PubMed:9573207). Plays a role as an electron transfer component for
CC both salicylate 5-hydroxylase (S5H) and naphthalene 1,2-dioxygenase
CC (NDO) systems, by transferring electrons to the oxygenase components
CC (PubMed:11872705, PubMed:9573207). {ECO:0000269|PubMed:11872705,
CC ECO:0000269|PubMed:9573207}.
CC -!- COFACTOR:
CC Name=[2Fe-2S] cluster; Xref=ChEBI:CHEBI:190135;
CC Evidence={ECO:0000250|UniProtKB:P0A185,
CC ECO:0000255|PROSITE-ProRule:PRU00628};
CC Note=Binds 1 [2Fe-2S] cluster per subunit.
CC {ECO:0000250|UniProtKB:P0A185, ECO:0000255|PROSITE-ProRule:PRU00628};
CC -!- PATHWAY: Aromatic compound metabolism; naphthalene degradation.
CC {ECO:0000269|PubMed:11872705, ECO:0000269|PubMed:9573207}.
CC -!- SUBUNIT: Ferredoxin NagAb belongs to both the salicylate 5-hydroxylase
CC (S5H) and the naphthalene 1,2-dioxygenase (NDO) multicomponent enzyme
CC systems. The NDO multicomponent enzyme system is composed of an
CC electron transfer component and a dioxygenase component (iron sulfur
CC protein (ISP)). The electron transfer component is composed of a
CC ferredoxin reductase (NagAa) and a ferredoxin (NagAb), and the
CC dioxygenase component is formed by a large alpha subunit (NagAc) and a
CC small beta subunit (NagAd). The S5H multicomponent enzyme system is
CC composed of an electron transfer component and a monooxygenase
CC component. The electron transfer component is composed of a ferredoxin
CC reductase (NagAa) and a ferredoxin (NagAb), and the monooxygenase
CC component is formed by a large subunit (NagG) and a small subunit
CC (NagH). {ECO:0000269|PubMed:11872705, ECO:0000269|PubMed:9573207}.
CC -!- DISRUPTION PHENOTYPE: Cells lacking this gene are not able to convert
CC salicylate to gentisate. {ECO:0000269|PubMed:9573207}.
CC -!- SIMILARITY: Belongs to the bacterial ring-hydroxylating dioxygenase
CC ferredoxin component family. {ECO:0000305}.
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DR EMBL; AF036940; AAD12609.1; -; Genomic_DNA.
DR AlphaFoldDB; O52381; -.
DR SMR; O52381; -.
DR UniPathway; UPA00082; -.
DR GO; GO:1902494; C:catalytic complex; IDA:UniProtKB.
DR GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0009055; F:electron transfer activity; IDA:UniProtKB.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0022900; P:electron transport chain; IDA:UniProtKB.
DR GO; GO:1901170; P:naphthalene catabolic process; IDA:UniProtKB.
DR GO; GO:0046244; P:salicylic acid catabolic process; IDA:UniProtKB.
DR Gene3D; 2.102.10.10; -; 1.
DR InterPro; IPR017941; Rieske_2Fe-2S.
DR InterPro; IPR036922; Rieske_2Fe-2S_sf.
DR Pfam; PF00355; Rieske; 1.
DR SUPFAM; SSF50022; SSF50022; 1.
DR PROSITE; PS51296; RIESKE; 1.
PE 1: Evidence at protein level;
KW 2Fe-2S; Aromatic hydrocarbons catabolism; Electron transport; Iron;
KW Iron-sulfur; Metal-binding; Plasmid; Transport.
FT CHAIN 1..104
FT /note="Naphthalene 1,2-dioxygenase/salicylate 5-hydroxylase
FT systems, ferredoxin component"
FT /id="PRO_0000421807"
FT DOMAIN 6..101
FT /note="Rieske"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00628"
FT BINDING 45
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000250|UniProtKB:P0A185,
FT ECO:0000255|PROSITE-ProRule:PRU00628"
FT BINDING 47
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000250|UniProtKB:P0A185,
FT ECO:0000255|PROSITE-ProRule:PRU00628"
FT BINDING 64
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000250|UniProtKB:P0A185,
FT ECO:0000255|PROSITE-ProRule:PRU00628"
FT BINDING 67
FT /ligand="[2Fe-2S] cluster"
FT /ligand_id="ChEBI:CHEBI:190135"
FT /evidence="ECO:0000250|UniProtKB:P0A185,
FT ECO:0000255|PROSITE-ProRule:PRU00628"
SQ SEQUENCE 104 AA; 11565 MW; BF761247B7579FF8 CRC64;
MTQNWIDAAC LDDIPEGDVV GVKVNGKEIA LYEVEGEIYA TDNLCTHGAA RMSDGFLEGR
EIECPLHQGR FDVCTGKALC TPLTKDIKTY PVKIENMRVM LKME
