ID   NAGB_LYSSH              Reviewed;         221 AA.
AC   P59686;
DT   23-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT   23-MAY-2003, sequence version 1.
DT   03-AUG-2022, entry version 59.
DE   RecName: Full=Glucosamine-6-phosphate deaminase;
DE            EC=3.5.99.6;
DE   AltName: Full=GlcN6P deaminase;
DE            Short=GNPDA;
DE   AltName: Full=Glucosamine-6-phosphate isomerase;
GN   Name=nagB;
OS   Lysinibacillus sphaericus (Bacillus sphaericus).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Lysinibacillus.
OX   NCBI_TaxID=1421;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=2362;
RX   PubMed=12855720; DOI=10.1099/mic.0.26231-0;
RA   Alice A.F., Perez-Martinez G., Sanchez-Rivas C.;
RT   "Phosphoenolpyruvate phosphotransferase system and N-acetylglucosamine
RT   metabolism in Bacillus sphaericus.";
RL   Microbiology 149:1687-1698(2003).
CC   -!- FUNCTION: Catalyzes the reversible isomerization-deamination of
CC       glucosamine 6-phosphate (GlcN6P) to form fructose 6-phosphate (Fru6P)
CC       and ammonium ion.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=alpha-D-glucosamine 6-phosphate + H2O = beta-D-fructose 6-
CC         phosphate + NH4(+); Xref=Rhea:RHEA:12172, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:28938, ChEBI:CHEBI:57634, ChEBI:CHEBI:75989; EC=3.5.99.6;
CC   -!- PATHWAY: Amino-sugar metabolism; N-acetylneuraminate degradation; D-
CC       fructose 6-phosphate from N-acetylneuraminate: step 5/5.
CC   -!- INDUCTION: By N-acetylglucosamine.
CC   -!- SIMILARITY: Belongs to the glucosamine/galactosamine-6-phosphate
CC       isomerase family. NagB subfamily. {ECO:0000305}.
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DR   EMBL; AY211495; AAO43398.1; -; Genomic_DNA.
DR   AlphaFoldDB; P59686; -.
DR   SMR; P59686; -.
DR   STRING; 1421.A2J09_05000; -.
DR   UniPathway; UPA00629; UER00684.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProt.
DR   GO; GO:0004342; F:glucosamine-6-phosphate deaminase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006044; P:N-acetylglucosamine metabolic process; IEA:InterPro.
DR   GO; GO:0019262; P:N-acetylneuraminate catabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd01399; GlcN6P_deaminase; 1.
DR   InterPro; IPR006148; Glc/Gal-6P_isomerase.
DR   InterPro; IPR004547; Glucosamine6P_isomerase.
DR   InterPro; IPR018321; Glucosamine6P_isomerase_CS.
DR   InterPro; IPR037171; NagB/RpiA_transferase-like.
DR   PANTHER; PTHR11280; PTHR11280; 1.
DR   Pfam; PF01182; Glucosamine_iso; 1.
DR   SUPFAM; SSF100950; SSF100950; 1.
DR   PROSITE; PS01161; GLC_GALNAC_ISOMERASE; 1.
PE   2: Evidence at transcript level;
KW   Carbohydrate metabolism; Hydrolase.
FT   CHAIN           1..221
FT                   /note="Glucosamine-6-phosphate deaminase"
FT                   /id="PRO_0000160132"
FT   ACT_SITE        49
FT                   /note="Proton acceptor; for enolization step"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        115
FT                   /note="For ring-opening step"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        117
FT                   /note="Proton acceptor; for ring-opening step"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        122
FT                   /note="For ring-opening step"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   221 AA;  24679 MW;  38B2D48971648DD3 CRC64;
     MHEPLPSLRL LNNGSTTFGL ATGGTMEPLY AKICKTDIDF SNCISFNLDE YVGLEANHEQ
     SYAYYMHQHL FHEKPFQASY LPNGLATNPL EEAARYEALL QQHSLDFQLL GIGQNGHIGF
     NEPGTSFESL THLVTLEEST RQANARFFSS INEVPTQAFT MGIQSIMRAK CILLIAVGET
     KREVLERVLA SDYTEEIPAS ALTKHPNVII LTDLQVEENK S