ID   NAGB_RALSP              Reviewed;         259 AA.
AC   O52384;
DT   28-NOV-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1998, sequence version 2.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=1,2-dihydroxy-1,2-dihydronaphthalene dehydrogenase {ECO:0000250|UniProtKB:P0A170};
DE            EC=1.3.1.29 {ECO:0000250|UniProtKB:P0A170};
DE   AltName: Full=Cis-naphthalene dihydrodiol dehydrogenase {ECO:0000303|PubMed:9573207};
GN   Name=nagB {ECO:0000312|EMBL:AAD12612.1};
OS   Ralstonia sp.
OG   Plasmid pWWU2 {ECO:0000312|EMBL:AAD12612.1}.
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Ralstonia.
OX   NCBI_TaxID=54061;
RN   [1] {ECO:0000305, ECO:0000312|EMBL:AAD12612.1}
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=U2 {ECO:0000312|EMBL:AAD12612.1};
RX   PubMed=9573207; DOI=10.1128/jb.180.9.2522-2530.1998;
RA   Fuenmayor S.L., Wild M., Boyes A.L., Williams P.A.;
RT   "A gene cluster encoding steps in conversion of naphthalene to gentisate in
RT   Pseudomonas sp. strain U2.";
RL   J. Bacteriol. 180:2522-2530(1998).
CC   -!- FUNCTION: Catalyzes the oxidation of naphthalene dihydrodiol into 1,2-
CC       dihydroxynaphthalene. {ECO:0000269|PubMed:9573207}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD(+) = H(+) + NADH
CC         + naphthalene-1,2-diol; Xref=Rhea:RHEA:11832, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17435, ChEBI:CHEBI:44343, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945; EC=1.3.1.29;
CC         Evidence={ECO:0000250|UniProtKB:P0A170};
CC   -!- PATHWAY: Aromatic compound metabolism; naphthalene degradation.
CC       {ECO:0000269|PubMed:9573207}.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000255}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF036940; AAD12612.1; -; Genomic_DNA.
DR   AlphaFoldDB; O52384; -.
DR   SMR; O52384; -.
DR   UniPathway; UPA00082; -.
DR   GO; GO:0018505; F:cis-1,2-dihydro-1,2-dihydroxynaphthalene dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR002347; SDR_fam.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; NAD; Oxidoreductase; Plasmid.
FT   CHAIN           1..259
FT                   /note="1,2-dihydroxy-1,2-dihydronaphthalene dehydrogenase"
FT                   /id="PRO_0000420237"
FT   ACT_SITE        153
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250|UniProtKB:Q48436"
FT   BINDING         8..35
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:Q48436"
FT   BINDING         58
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:Q48436"
FT   BINDING         140
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250|UniProtKB:Q48436"
FT   BINDING         157
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250|UniProtKB:Q48436"
SQ   SEQUENCE   259 AA;  27062 MW;  50011B273350BFD5 CRC64;
     MNIQQVIAIT GAGSGIGLEL VRSFKAAGYC VSALVRNEEQ EAGLRSEFKD AIEIVAGDVC
     DHATNEKLVN KAVARFGHLD CFIGNAGIWD YMLGVDEPWE KLSGSFEEIF DINVKSYFSG
     ISAALPELKK TNGSVVVTAS VSSYAAGGGG SCYIASKHAV LGMVKALAYE LAPHIRVNGV
     APGGTVTSLA GPASAGFDKT KMKDMPGIDD MIKGLTPLGF AARPEDVVAP YLLLASREQG
     KFITGTVIGI DGGMALGRK