ID   NAGC_ECOLI              Reviewed;         406 AA.
AC   P0AF20; P15301;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2005, sequence version 1.
DT   25-MAY-2022, entry version 113.
DE   RecName: Full=N-acetylglucosamine repressor;
GN   Name=nagC; Synonyms=nagR; OrderedLocusNames=b0676, JW0662;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=2668691; DOI=10.1111/j.1365-2958.1989.tb00197.x;
RA   Plumbridge J.;
RT   "Sequence of the nagBACD operon in Escherichia coli K12 and pattern of
RT   transcription within the nag regulon.";
RL   Mol. Microbiol. 3:505-515(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=2190615; DOI=10.1139/o90-017;
RA   Peri K.G., Goldie H., Waygood E.B.;
RT   "Cloning and characterization of the N-acetylglucosamine operon of
RT   Escherichia coli.";
RL   Biochem. Cell Biol. 68:123-137(1990).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA   Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA   Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA   Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA   Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA   Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA   Horiuchi T.;
RT   "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT   the 12.7-28.0 min region on the linkage map.";
RL   DNA Res. 3:137-155(1996).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [6]
RP   FUNCTION.
RX   PubMed=7545108; DOI=10.1002/j.1460-2075.1995.tb00067.x;
RA   Plumbridge J.;
RT   "Co-ordinated regulation of amino sugar biosynthesis and degradation: the
RT   NagC repressor acts as both an activator and a repressor for the
RT   transcription of the glmUS operon and requires two separated NagC binding
RT   sites.";
RL   EMBO J. 14:3958-3965(1995).
CC   -!- FUNCTION: Acts as a repressor of the nagEBACD operon and acts both as
CC       an activator and a repressor for the transcription of the glmSU operon.
CC       {ECO:0000269|PubMed:7545108}.
CC   -!- INDUCTION: By N-acetylglucosamine.
CC   -!- SIMILARITY: Belongs to the ROK (NagC/XylR) family. {ECO:0000305}.
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DR   EMBL; X14135; CAA32354.1; -; Genomic_DNA.
DR   EMBL; AF052007; AAC09326.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC73770.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAA35319.1; -; Genomic_DNA.
DR   PIR; C64802; C64802.
DR   RefSeq; NP_415202.1; NC_000913.3.
DR   RefSeq; WP_000187594.1; NZ_STEB01000044.1.
DR   AlphaFoldDB; P0AF20; -.
DR   SMR; P0AF20; -.
DR   BioGRID; 4261210; 25.
DR   DIP; DIP-35984N; -.
DR   IntAct; P0AF20; 9.
DR   STRING; 511145.b0676; -.
DR   jPOST; P0AF20; -.
DR   PaxDb; P0AF20; -.
DR   PRIDE; P0AF20; -.
DR   EnsemblBacteria; AAC73770; AAC73770; b0676.
DR   EnsemblBacteria; BAA35319; BAA35319; BAA35319.
DR   GeneID; 66671054; -.
DR   GeneID; 945285; -.
DR   KEGG; ecj:JW0662; -.
DR   KEGG; eco:b0676; -.
DR   PATRIC; fig|1411691.4.peg.1602; -.
DR   EchoBASE; EB0630; -.
DR   eggNOG; COG1846; Bacteria.
DR   eggNOG; COG1940; Bacteria.
DR   HOGENOM; CLU_036604_13_1_6; -.
DR   InParanoid; P0AF20; -.
DR   OMA; VDFGHTH; -.
DR   PhylomeDB; P0AF20; -.
DR   BioCyc; EcoCyc:PD00266; -.
DR   PRO; PR:P0AF20; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0003677; F:DNA binding; IDA:EcoCyc.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:EcoCyc.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR043129; ATPase_NBD.
DR   InterPro; IPR000835; HTH_MarR-typ.
DR   InterPro; IPR000600; ROK.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   PANTHER; PTHR18964; PTHR18964; 1.
DR   Pfam; PF01047; MarR; 1.
DR   Pfam; PF00480; ROK; 1.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   SUPFAM; SSF53067; SSF53067; 1.
DR   PROSITE; PS01125; ROK; 1.
PE   2: Evidence at transcript level;
KW   Activator; Carbohydrate metabolism; DNA-binding; Reference proteome;
KW   Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..406
FT                   /note="N-acetylglucosamine repressor"
FT                   /id="PRO_0000095691"
FT   DNA_BIND        35..44
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        203..204
FT                   /note="EH -> DD (in Ref. 1; CAA32354)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   406 AA;  44541 MW;  28C4C85D736627C8 CRC64;
     MTPGGQAQIG NVDLVKQLNS AAVYRLIDQY GPISRIQIAE QSQLAPASVT KITRQLIERG
     LIKEVDQQAS TGGRRAISIV TETRNFHAIG VRLGRHDATI TLFDLSSKVL AEEHYPLPER
     TQQTLEHALL NAIAQFIDSY QRKLRELIAI SVILPGLVDP DSGKIHYMPH IQVENWGLVE
     ALEERFKVTC FVGHDIRSLA LAEHYFGASQ DCEDSILVRV HRGTGAGIIS NGRIFIGRNG
     NVGEIGHIQV EPLGERCHCG NFGCLETIAA NAAIEQRVLN LLKQGYQSRV PLDDCTIKTI
     CKAANKGDSL ASEVIEYVGR HLGKTIAIAI NLFNPQKIVI AGEITEADKV LLPAIESCIN
     TQALKAFRTN LPVVRSELDH RSAIGAFALV KRAMLNGILL QHLLEN