ID   NAGS_PICST              Reviewed;         581 AA.
AC   A3GG03;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   19-JAN-2010, sequence version 2.
DT   25-MAY-2022, entry version 71.
DE   RecName: Full=Amino-acid acetyltransferase, mitochondrial;
DE            EC=2.3.1.1;
DE   AltName: Full=Arginine-requiring protein 2;
DE   AltName: Full=Glutamate N-acetyltransferase;
DE   AltName: Full=N-acetylglutamate synthase;
DE            Short=AGS;
DE            Short=NAGS;
DE   Flags: Precursor;
GN   Name=ARG2; ORFNames=PICST_51037;
OS   Scheffersomyces stipitis (strain ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL
OS   Y-11545) (Yeast) (Pichia stipitis).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Debaryomycetaceae; Scheffersomyces.
OX   NCBI_TaxID=322104;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 58785 / CBS 6054 / NBRC 10063 / NRRL Y-11545;
RX   PubMed=17334359; DOI=10.1038/nbt1290;
RA   Jeffries T.W., Grigoriev I.V., Grimwood J., Laplaza J.M., Aerts A.,
RA   Salamov A., Schmutz J., Lindquist E., Dehal P., Shapiro H., Jin Y.-S.,
RA   Passoth V., Richardson P.M.;
RT   "Genome sequence of the lignocellulose-bioconverting and xylose-fermenting
RT   yeast Pichia stipitis.";
RL   Nat. Biotechnol. 25:319-326(2007).
CC   -!- FUNCTION: N-acetylglutamate synthase involved in arginine biosynthesis.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=acetyl-CoA + L-glutamate = CoA + H(+) + N-acetyl-L-glutamate;
CC         Xref=Rhea:RHEA:24292, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985,
CC         ChEBI:CHEBI:44337, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.1;
CC   -!- PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl-
CC       L-ornithine from L-glutamate: step 1/4.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the acetyltransferase family. {ECO:0000305}.
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DR   EMBL; AAVQ01000001; EAZ63850.2; -; Genomic_DNA.
DR   RefSeq; XP_001387873.2; XM_001387836.1.
DR   AlphaFoldDB; A3GG03; -.
DR   SMR; A3GG03; -.
DR   STRING; 4924.XP_001387873.2; -.
DR   EnsemblFungi; EAZ63850; EAZ63850; PICST_51037.
DR   GeneID; 4851202; -.
DR   KEGG; pic:PICST_51037; -.
DR   eggNOG; KOG2436; Eukaryota.
DR   HOGENOM; CLU_013088_0_0_1; -.
DR   InParanoid; A3GG03; -.
DR   OMA; WAMFWTT; -.
DR   OrthoDB; 769117at2759; -.
DR   UniPathway; UPA00068; UER00106.
DR   Proteomes; UP000002258; Chromosome 1.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0004042; F:acetyl-CoA:L-glutamate N-acetyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0103045; F:methione N-acyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006526; P:arginine biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR011190; GlcNAc_Synth_fun.
DR   InterPro; IPR006855; Vertebrate-like_GNAT_dom.
DR   Pfam; PF04768; NAT; 1.
DR   PIRSF; PIRSF007892; NAGS_fungal; 1.
DR   PROSITE; PS51731; GNAT_NAGS; 1.
PE   3: Inferred from homology;
KW   Acyltransferase; Amino-acid biosynthesis; Mitochondrion;
KW   Reference proteome; Transferase; Transit peptide.
FT   TRANSIT         1..?
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           ?..581
FT                   /note="Amino-acid acetyltransferase, mitochondrial"
FT                   /id="PRO_0000372575"
FT   DOMAIN          401..558
FT                   /note="N-acetyltransferase"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00532"
SQ   SEQUENCE   581 AA;  66705 MW;  8ADAA34E3377BA1B CRC64;
     MSKLKNLNRE FISNLKSHKL ITDAKRNLIL SILKSTTTKR EARNYLNKYQ NQFDFGDLKI
     SSSAKYEQDV SKLTKRDSQR ELFVNRYLNK QNPFINIYDD ETKLKKIPLR VALFKLKFLN
     IDPKEWRGIA ETFKRLVNLG ISPIVFLDYD HLPTDSFKYN ELYMINQVNK VMNYLGKPEE
     EGNLKTTVLR SLFTVENKER GPVINSLESI LIPLYQGIIP FIQPIIYNAE STFQQFINSN
     QLLYSLCESL LDKKDLLSVE KIVMIDPIGG IPSVERNQTS HVFINLSQEY SDIVSELYIG
     HIEPDQRDLH LANLNTMHEI LTLASSKSGN DDTTGIITTP FIMSVNDDLI NPIIYNVLTD
     RPIISSSLPS SNNRTPQLST SILKKGVDVR SYDADNYARK FTLHNLIEDE LVDKNRLVAL
     LDDSFGKNLD TDSYFDRINN SLATLVIVGD YDGAAIITWE YSGTNKIAYL DKFAIAKKNQ
     GLPGLADVIF KIILSSHPHE LIWRSRKVNP VNKWYFERCV GSMSSPESQW RIFYTGDIFN
     RRIDKRRKRI VGSEAVNISD KLVQYSEICE GIPPSFFSSK E