ID   NAHB_PSEPU              Reviewed;         259 AA.
AC   P0A169; O33462; Q52459;
DT   01-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 61.
DE   RecName: Full=1,2-dihydroxy-1,2-dihydronaphthalene dehydrogenase;
DE            EC=1.3.1.29;
DE   AltName: Full=Cis-naphthalene dihydrodiol dehydrogenase;
DE   AltName: Full=Dibenzothiophene dihydrodiol dehydrogenase;
DE            EC=1.3.1.60;
GN   Name=nahB;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OG   Plasmid NPL1.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=BS202;
RA   Bezborodnikov S.G., Boronin A.M., Tiedje J.M.;
RT   "Nucleotide sequences of genes encoding an upper pathway of naphthalene
RT   metabolism of NPL1 plasmid from Pseudomonas putida strain BS202.";
RL   Submitted (JUL-1997) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the oxidation of naphthalene dihydrodiol into 1,2-
CC       dihydroxynaphthalene. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(1R,2S)-1,2-dihydronaphthalene-1,2-diol + NAD(+) = H(+) + NADH
CC         + naphthalene-1,2-diol; Xref=Rhea:RHEA:11832, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:17435, ChEBI:CHEBI:44343, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945; EC=1.3.1.29;
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=cis-1,2-dihydroxy-1,2-dihydrodibenzothiophene + NAD(+) = 1,2-
CC         dihydroxydibenzothiophene + H(+) + NADH; Xref=Rhea:RHEA:24188,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:16941, ChEBI:CHEBI:17212,
CC         ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.3.1.60;
CC   -!- PATHWAY: Aromatic compound metabolism; naphthalene degradation.
CC   -!- SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR)
CC       family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF010471; AAB62709.1; -; Genomic_DNA.
DR   AlphaFoldDB; P0A169; -.
DR   SMR; P0A169; -.
DR   UniPathway; UPA00082; -.
DR   GO; GO:0018505; F:cis-1,2-dihydro-1,2-dihydroxynaphthalene dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0018513; F:dibenzothiophene dihydrodiol dehydrogenase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019439; P:aromatic compound catabolic process; IEA:UniProtKB-KW.
DR   InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR   InterPro; IPR002347; SDR_fam.
DR   PRINTS; PR00081; GDHRDH.
DR   PRINTS; PR00080; SDRFAMILY.
DR   SUPFAM; SSF51735; SSF51735; 1.
PE   3: Inferred from homology;
KW   Aromatic hydrocarbons catabolism; NAD; Oxidoreductase; Plasmid.
FT   CHAIN           1..259
FT                   /note="1,2-dihydroxy-1,2-dihydronaphthalene dehydrogenase"
FT                   /id="PRO_0000054730"
FT   ACT_SITE        153
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   BINDING         8..32
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
FT   BINDING         140
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   259 AA;  27518 MW;  187E517F5AA89152 CRC64;
     MGNQQVVSIT GAGSGIGLEL VRSFKSAGYY VSALVRNEEQ EALLCKEFKD ALEIVVGDVR
     DHATNEKLIK QTIDRFGHLD CFIANAGIWD YMLSIEEPWE KISSSFDEIF DINVKSYFSG
     ISAALPELKK TNGSVVMTAS VSSHAVGGGG SCYIASKHAV LGMVKALAYE LAPEVRVNAV
     SPGGTVTSLC GSASAGFDKM HMKDMPGIDD MIKGLTPLGF AAKPEDVVAP YLLLASRKQG
     KFITGTVISI DGGMALGRK